Effects of light regulation on the synthesis of secondary metabolites in medicinal plants / 药学实践杂志

Secondary metabolites of medicinal plants are extremely important to human health because of their special pharmacological activities or efficacy. They are the main source of drugs, health care products, and cosmetics. As human beings continue to pursue health and longevity, the demand in the pharmaceutical market continues to grow. It becomes especially important to improve the production and quality of secondary metabolites of medicinal plants. Plant secondary metabolites are a kind of adaptation of plants to their environment and are the result of the interaction between plants and biotic and abiotic factors during the long-term evolution process. The production and accumulation of secondary metabolites in medicinal plants are mainly affected by plant genetic factors and environmental factors. Among them, light environment is extremely important for their synthesis. Therefore, light regulation has long been a research focus for many scholars in China and abroad. In this article, we the recent research progress on the effects of light regulation on the secondary metabolites of medicinal plants were reviewed, mainly focusing on the effects of light quality, light intensity and photoperiod, in order to provide theoretical basis and practical guidance for the efficient production of secondary metabolites with important pharmacological activities.

Red light enhances folate accumulation in wheat seedlings / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

Red, white, blue, green, and yellow lights were applied to investigate their effects on folate accumulation in wheat seedlings. The different lights, especially red light, significantly increased the total folate content. Total folate showed maximum accumulation under 30 μmol/(m

Biotechnological Assessment of Culture Conditions on the Stress-Induced Carotenoid Production of Dunaliella salina and Growth Kinetics of Chlorophyceae Microalgae Strains

Abstract Microalgae are potential sources of a wide range of bioproducts. It is essential to choose the proper microalgae strain and culture condition to achieve an efficient production. The production yield of carotenoids by Dunaliella salina under the stress-induced culture conditions of nitrogen deprivation and excessive light intensity was evaluated. Also, a survey at laboratorial scale of the growth kinetics under different culture conditions of photoperiod, aeration, and agitation was performed for the seven species of green microalgae Ankistrodesmus fusiformis, Chlamydocapsa bacillus, Desmodesmus brasiliensis, Kirchneriella lunaris, Pseudokirchneriella subcapitata, and Scenedesmus obliquus. As a result, aeration of atmospheric air is enough to improve the growth kinetics of the seven species studied. Production of carotenoids was enhanced under stress by excessive light intensity. Although D. salina does not grow effectively under nitrogen deprivation, this stress condition may be used to quickly stimulate carotenoid production once the culture reaches a high cellular population.

Effects of light quality on growth and icariin flavonoid content of Epimedium pseudowushanense under different light intensity / 中国中药杂志

In this study, the growth index including plant height, compound leaf area, specific leaf area, leaf water content, number of branches, and leaf biomass per plant and the icariin flavonoids such as epimedin A, epimedin B, epimedin C and icariin of Epimedium pseudowushanense were determined on 30 d and 60 d under light intensity(18.2±2.5) μmol·m~(-2)·s~(-1)(L1) and(90.9 ±2.5) μmol·m~(-2)·s~(-1)(L2), and white light as control, red light, blue light and yellow light were used as three light quality treatments, to study the effect of light quality on the growth and flavonoids accumulation of E. pseudowushanense. The E. pseudowushanense was sui-table for growth under L1 light intensity, the blue light treatment significantly reduced the leaf area, but had little effect on the stem height, the red light treatment and the yellow light treatment had no obvious effect on the stem height and leaf area, but the yellow light treatment significantly increased the germination of new branches, and had a sustained promoting effect, and the biomass was significantly higher than the white light treatment at 60 d. The content of icariin flavonoids in red light, blue light and yellow light treatment was higher than that in white light treatment at 30 d and 60 d under L1 light intensity, while yellow light treatment promoted the synthesis of icariin flavonoids to the largest extent, which was 1.8 and 1.9 times of white light treatment(30 d and 60 d).Under L2 light intensity, the effect of strong light on promoting stem germination became the main factor, while the yellow light treatment showed no significant effect on promoting stem germination, and the red light treatment exhibited a significant effect on reducing leaf area. Icariin flavonoids under red light, blue light and yellow light treatment were all lower than that under white light treatment, that is, the effect of white light treatment on the synthesis of icariin flavonoids is better than red light, blue light and yellow light treatment. When the time of strong light treatment was longer, the degradation range of icariin flavonoids in other light treatment appeared, while red light treatment promotes the synthesis of icariin flavonoids. Therefore, the influence of light quality on E. pseudowushanense is quite different under different light intensity, no matter from growth index or flavonoid content index. The results support that the biomass and icariin flavonoid content can be increased by providing appropriate red and yellow light.

Effects of light intensity on material and energy metabolism of Viola yedoensis / 中国中药杂志

Light energy is an important factor affecting plant growth. The hypothesis of "light-cold and heat property" holds that the original plants of traditional Chinese medicines(TCM) with cold property can obtain more energy to maintain growth in high light intensity environment, whereas the original plants of TCM with heat property prefer weak light environment. The current experiment investigated the effects of different light intensities on primary metabolites levels, energy levels, cell apoptosis, and leaves ultrastructure of Viola yedoensis, the original plants of TCM Violae Herba with cold property. There were five treatment groups of V. yedoensis, which was planted under Li1(8 500 lx),Li2(7 250 lx),Li3(6 000 lx),Li4(4 750 lx),Li5(3 500 lx)LEDs light intensity conditions, respectively. After harvest, primary metabolites levels, contents of ATP, ADP, AMP, activities of ATP synthesis and hydrolysis related enzyme, as well as cell apoptosis activation degree were measured, and transmission electron microscopy technique was used to observe leaves ultrastructure. The results showed that the total sugar, total protein, contents of ATP, ADP and AMP, activities of NADH dehydrogenase, cytochrome C reductase, ATP synthase and ATP hydrolase were positively correlated with light intensities(P<0.05). The crude fat content, activities of SDH and CCO enzyme showed a trend of increasing first and then decreasing, the highest value were found in Li2 group and Li3 group respectively(P<0.05). The vitality of caspase-3 and caspase-9 was negatively correlated with light intensities(P<0.05). The structure of chloroplast and mitochondria were normal and intact in Li1-Li3 groups, and the damage degree of Li4 and Li5 groups increased with the decrease of light intensities. The analysis of results indicated that the structure of chloroplast and mitochondria of V. yedoensis is normal under the light intensity of 6 000-8 500 lx, which can obtain more energy to maintain its growth and metabolism. When the light intensity is lower than 4 750 lx, the chloroplast morphology and mitochondrial membrane are damaged, affecting the metabolism of material and energy. There was no significant difference in energy charge of V. yedoensis in the light intensity range of 3 500～8 500 lx. The effect of light intensity on energy metabolism of V. yedoensis accords with the hypothesis of "light-cold and heat property".

Vegetative growth and yield of robusta coffee genotypes cultivated under different shading levels / Crescimento vegetativo e produção de genótipos de café robusta cultivados sob diferentes níveis de sombra

The growing in shaded systems is one of the strategies adopted to mitigate the impacts of climate change on coffee trees. However, there are few studies about the effect of shading on the growth and production of clones of robusta coffee (Coffea canephora). Besides that, exist a paradigm in Brazil of that the shading in coffee robusta is synonymous of less yield. Thus, the aim of this work was to evaluate whether C. canephora genotypes subjected to shading increase their yield and growth vegetative under field conditions. The study was performed between September 2013 and July 2017 (four harvests) in Alegre, Espírito Santostate, Brazil. Three genotypes of cultivar Conilon Vitória - Incaper 8142 were evaluated (03V, 06V, and 12V) and four levels of shade: full sunlight (witness) and, low, moderate and, high shade, with 38, 48 and, 68% of real capacity of shade, respectively. The shade was performed using black polyolefin screens; plants growing in full sunlight were only fenced. The tested shade levels affected the growth rate and yield of Robusta coffee plants. The shading caused a greater leaf expansion as compared to the full sunlight, as well as greater etiolation of the plagiotropic and orthotropic branches. The genotype 06V was shown to be responsive to shading, with a statistically equal production independently of shade level. The low, moderate, and high shade levels affected negatively the yield of genotypes 03V and 12V. The shading can improve the yield of Robusta coffee, in this case, of the genotype 06V of the cultivar Conilon Vitória - Incaper 8142. The success of shaded plantations depends the choice of the shade responsive genotypes and appropriate shading level.

O cultivo em sistemas sombreados é uma das estratégias adotadas para mitigar os impactos da mudança climática sobre os cafeeiros. No entanto, existem poucos estudos sobre o efeito do sombreamento no crescimento e produção de clones de café robusta (Coffea canephora). Além disso, existe um paradigma no Brasil de que o sombreamento nesta espécies é sinônimo de menor rendimento. Assim, o objetivo do presente trabalho foi avaliar se os genótipos de C. canephora submetidos ao sombreamento aumentam sua produtividade e crescimento vegetativo em condições de campo. O estudo foi realizado entre setembro de 2013 e julho de 2017 (quatro safras) em Alegre, Espírito Santo, Brasil. Três genótipos da cultivar Conilon Vitória - Incaper 8142 foram avaliados (03V, 06V e 12V) e quatro níveis de sombreamento: pleno sol (testemunha) e baixo,moderado e alto nível de sombreamento, com 34, 48 e 68% de capacidade real de sombreamento, respectivamente, obtidos com o uso de telas de poliolefinas de cor preta; plantas cultivadas em pleno sol foram apenas cercadas. Os níveis de sombreamento testados afetaram a taxa de crescimento e produtividade dasplantas de café Robusta O sombreamento provocou maior expansão foliar em relação ao pleno sol, bem como maior estiolação dos ramos plagiotrópicos e ortotrópicos. Os baixos, moderados e altos níveis de sombreamento afetaram negativamente a produção dos genótipos 03V e 12V. O sombreamento pode melhorar o rendimento do café Robusta, neste caso, do genótipo 06V da cultivar Conilon Vitória - Incaper 8142. O sucesso dasplantações cafeeiras sombreadas depende da escolha dos genótipos responsivos à sombra e do nível apropriado de sombreamento.

Effects of light intensities on growth,physiological characteristic and chemical composition of Viola yedoensis / 中国中药杂志

The study is aimed to investigate the effects of light intensities on growth,photosynthetic physiology,antioxidant systems and chemical composition of Viola yedoensis and provide cultivation references for V.yedoensis.Five groups of V.yedoensis were planted under five light intensities conditions,namely 100%,80%,50%,35%,5%of full sunlight,and then morphological index,growth,chlorophyll fluorescence parameters,photosynthetic parameters and antioxidant enzyme system indexes were measured during harvest.The results showed that there was no significant difference in the biomass of V.yedoensis among 35% -100%full sunlight,but the biomass of those were significantly higher than that in the 5%full sunlight treatment(P<0.05).The net photosynthetic rate,transpiration rate,stomatal conductance,intercellular CO_2 concentration and water use efficiency increased firstly and then decreased with the decrease of light intensity;F_m,F_v/F_mand Yield in 5% full sunlight treatment were significantly lower than those in the other four groups(P<0.05).The structure of chloroplast was normal under light intensity ranged from 50%to 100% full sunlight.The lamellar concentration of chloroplast matrix decreased and the starch granules decreased in 35% full sunlight treatment,and the margin of lamellar layer of chloroplast and substrate were blurred,and the starch granules were small and the number of starch granules decreased significantly under 5% full sunlight.MDA content in 5%full sunlight treatment was significantly higher than those in the other four groups(P<0.05).The total coumarin content and total flavonoid content decreased with the decrease of light intensity.In summary,the light in-tensity range suitable for the growth of V.yedoensis is wide(ranging from 35% to 100% full sunlight).The content of flavonoids and coumarins is positively correlated with light intensity.

Effect of light intensity on expression of gonadotropin-releasing hormone in mesencephalon and diencephalon of Jinghong-1 hens / 解剖学报

Objective To explore the nervous regulating mechanism in the effect of light intensity on reproductive performance. Methods Sixty healthy Jinghong-1 hens aged 100 days were randomly divided into four groups of 15 hens and applied a light of 30 lx, 20 lx, 10 lx, and 1 lx, respectively, for 7 days. The distribution and expression of gonadotropin-releasing hormone (GnKH) in mesencephalon and diencephalon were detected using immunohistochemical and image analysis methods. Results GnKH immunoreactive positive neurons were mainly observed in stratum griseum centrale (SCC) of tectum, nucleus isthmi pars magnocellularis (Imc) , nucleus isthmi pars parvocellularis (Ipc) , nucleus subpre-tectalis/interstito-pretectalis-subpretectalis (SP/IPS), nucleus of basal optic root (nbor), and nucleus semilunaris (Slu) of mesencephalon. The mean gray values of positive neurons in 30 lx and 20 lx groups were significantly higher than those of 10 lx and 1 lx groups (P0. 05) between 30 lx and 20 lx groups, but they were significant higher than those of 10 lx and 1 lx (P<0. 05) in PHN, PVN, and GLv. The percent cell area in Hot was the largest in 30 lx group, having no significant difference comparing 20 Ix, being significant higher than those of 10 lx and I Ix (P<0. 05). Those in 30 lx and 20 lx groups were significant higher than 10 lx and 1 lx (P<0.05) in PHN and PVN. Conclusion Light intensity affect the expression of GnRH in hen mesencephalon and diencephalon obviously in Jinghong-1 hens aged 100 days, regulating their reproductive function. The tectofugal pathway of the optical pathways, including collateral circulation of nucleus isthmi, plays an important role in the course of regulation.

Effects of light intensity on growth and content of active components of Uncaria rhynchophyll / 中国中药杂志

The aim of this study was to study the effects of different light intensity on the growth,biomass accumulation and distribution,chlorophyll content and effective components of Uncaria rhynchophylla seedlings,and explore the suitable light intensity conditions for artificial cultivation of U. rhynchophylla seedlings. Three-year-old U. rhynchophylla seedlings were used as experimental materials. Four light intensity levels( 100%,70%-75%,30%-35%,5%-10%) were set up with different layers of black shading net. With the decrease of light intensity,the results showed that the plant height,basal diameter and biomass( root,stem,leaf and hook) of U.rhynchophylla seedlings exhibited the trend of " increase-decrease". Under 70%-75% light intensity,the plant height,basal diameter,biomass( root,stem,leaf,hook) of U. rhynchophylla seedlings were significantly higher than those of other treatments( P< 0. 05);under 5%-10% light intensity,the plant height,basal diameter and biomass( root,hook) of U. rhynchophylla seedlings were significantly lower than those of other treatments( P<0. 05). With the decrease of light intensity,the chlorophyll content of U. rhynchophylla seedlings increased gradually: under 100% light intensity,the chlorophyll content of U. rhynchophylla seedlings were the smallest,while under 100% light intensity,its chlorophyll content was the highest. With the decrease of light intensity,the contents of rhynchophylline and isorhynchophylline in different organs of U. rhynchophylla seedlings varied: under 30%-35% light intensity,the contents of rhynchophylline and isorhynchophylline in hooks and rhynchophylline content in stems were the highest; under 5%-10% light intensity,the contents of rhynchophylline and isorhynchophylline in leaves and stems of U. rhynchophylla were the highest. In conclusion,70%-75% light intensity is suitable for seedling growth and biomass accumulation,and 30%-35% light intensity is suitable for the accumulation of effective components in U. rhynchophylla seedlings.

Effects of red light and blue light on root morphology and accumulation of bioactive compounds in Salvia miltiorrhiza / 中草药

Objective: Investigate the effects of different intensity of white light, red light and blue light on root morphology and bioactive compounds accumulations of Salvia miltiorrhiza. Methods: The seedlings of S. miltiorrhiza were treated with 4 intensities of white light, red light and blue light, respectively, the morphologic index of root such as the lateral root number, lateral root diameter as well as the content of seven bioactive compounds were determined. Results: The influences of different light quality on the above-ground morphological indexes of S. miltiorrhiza were distinct. Red light of 100 μmol/(m2∙s) and 200 μmol/(m2∙s) promoted the occurrence of lateral root, and blue light of 200 μmol/(m2∙s) and 300 μmol/(m2∙s) significantly increased the root biomass. The accumulation of different bioactive compounds was distinctly affected by light quality and light intensity, the accumulation of tanshinones was more sensitive to the light treatments than that of phenolic acids. Conclusion: Light regulates the lateral root development and accumulations of bioactive compounds simultaneously, the application of artificial light is a promising method to improve the yield and quality of S. miltiorrhiza.

Effects of light quality and intensity on growth, related enzymes activity, and components of Polygala tenuifolia / 中草药

Objective To study the growth physiology and components of Polygala tenuifolia under different light quality and intensity, and provide theoretical basis for the artificial cultivation, yield improvement, and increasing the target composition of P. tenuifolia. Methods Using P. tenuifolia shoots as the experimental material, four kinds of ights (white, yellow, red, and blue) as light quality, the light intensity was set at 100, 300, and 500 μmol/(m2∙s) to determinate the plant height, root length, leaf length, leaf width, malondialdehyde (MDA) content, antioxidant enzyme activity, and secondary metabolite content in P. tenuifolia after 30 d of illumination. Results Under the red light, P. tenuifolia had the highest plant height, the longest root length and leaf length, and the largest biomass. Under the yellow light, the MDA content was the lowest, and the MDA content was the highest under white light. The activities of SOD, POD, and CAT were significantly increased under red and blue light. The highest content of total flavonoids, total phenols, and 3,6'-disinapoyl sucrose under blue light. Different light qualities had no significant effect on the content of polygalaxanthone III. The biomass of plant height and the 3,6’-disinapoyl sucrose content were the highest under the light intensity of 300 μmol/(m2∙s). P. tenuifolia had the longest roots under the light intensity of 500 μmol/(m2∙s), and the content of MDA and the activities of POD, SOD, and CAT increased with the increase of light intensity. Conclusion Red light has the strongest promoting effect on the roots of P. tenuifolia. Red and blue light significantly increases the activity of POD, SOD, and CAT. Blue light promotes the accumulation of total flavonoids, total phenols, and 3,6’-disinapoyl sucrose. The light intensity of 500 μmol/(m2∙s) has the strongest promoting effect on the roots of P. tenuifolia, and the activities of POD, SOD, and CAT in leaves were higher. Different light qualities had no significant effect on the contents of total flavonoids, total phenols, and polygalaxanthone III. The light intensity of 300 μmol/(m2∙s) promotes the accumulation of 3,6’-disinapoyl sucrose.

The effects of shade and light irradiation on the polymerization of composite resin core materials / 实用口腔医学杂志

Objective:To evaluate the effects of shade and light irradiation condition on the polymerization of composite resin core material.Methods:The light-proof silicon rubber cuboid mold with one end open were stringed and filled by dual-cured flowable composite resin core material with the shade of dentine and white(Paracore) respectively,then the open end of mold was irradiated directly by a light unit at 1 000 mW/cm2 ×20 s and 3 200 mW/cm2 ×6 s respectively.And all specimens of 4 groups(n =5) were stored in the light-proof box.1 h and 24 h after irradiation,Knoop microhardness was measured along the vertical surfaces of the specimens at the depth of 1,2 and 3 mm respectively.Data were analyzed by One-way ANOVA.Results:At all light irradiation conditions,the hardness of all specimens decreased with the increase of measurement depth.24 h after irradiation,the hardness of dentine shade specimens at each measurement depth showed no difference between 1 000 mW/cm2 × 20 s and 3 200 mW/cm2 × 6 s irradiation,while the white shade specimens irradiated by 1 000 mW/cm2 × 20 s showed higher hardness than those by 3 200 mW/cm2 × 6 s.Conclusion:To obtain sufficient polymerization of composite core material with different shade optimal light irradiation condition should be selected.

Influence of light intensity and water content of medium on total dendrobine of Dendrobium nobile Lindl

Objective To ascertain the influence of light intensity and water content of medium on the total dendrobine of Dendrobium nobile (D. nobile). Method The principal component analysis combined with total dendrobine accumulation was conducted to assess the yield and quality of D. nobile in all treatments. In the experiment, D. nobile plants were cultivated in greenhouse as tested materials, and complete test of 9 treatments was adopted with relative light intensities 75.02%, 39.74%, 29.93% and relative water content of medium 50%, 65%, 80%. The plants were treated in June and harvested till December. Indexes including agronomic traits, fresh weight and dry weight of stem and leaf, ash content, extract, and dendrobine were measured. Results Under the light intensity treatments of 75.02% with 50%, 65%, 80% water content of medium, the basal stems of plants were comparatively thicker with more leaves, and the fresh weight and dry weight of stems and leaves were significantly higher than other 6 treatments. Leaves in all treatments contained dendrobine. Under the light intensity treatments of 75.02% with 50%, 65%, 80% water content of medium, dendrobine content of leaves was lower while dendrobine contents of other treatments were more than 0.60%. After comprehensive assessment through the principal component analysis and total dendrobine accumulation, the results showed that 3 treatments with relative light intensity of 75.02% ranked the top three. Conclusions In brief, the moderately strong light intensity and water content of medium from low to medium can facilitate the growth and yield of D. nobile plants, while light intensity from moderately weak to weak can enhance the dendrobine content.

Influence of light intensity and water content of medium on total dendrobine of Dendrobium nobile Lindl

OBJECTIVE@#To ascertain the influence of light intensity and water content of medium on the total dendrobine of Dendrobium nobile (D. nobile).@*METHOD@#The principal component analysis combined with total dendrobine accumulation was conducted to assess the yield and quality of D. nobile in all treatments. In the experiment, D. nobile plants were cultivated in greenhouse as tested materials, and complete test of 9 treatments was adopted with relative light intensities 75.02%, 39.74%, 29.93% and relative water content of medium 50%, 65%, 80%. The plants were treated in June and harvested till December. Indexes including agronomic traits, fresh weight and dry weight of stem and leaf, ash content, extract, and dendrobine were measured.@*RESULTS@#Under the light intensity treatments of 75.02% with 50%, 65%, 80% water content of medium, the basal stems of plants were comparatively thicker with more leaves, and the fresh weight and dry weight of stems and leaves were significantly higher than other 6 treatments. Leaves in all treatments contained dendrobine. Under the light intensity treatments of 75.02% with 50%, 65%, 80% water content of medium, dendrobine content of leaves was lower while dendrobine contents of other treatments were more than 0.60%. After comprehensive assessment through the principal component analysis and total dendrobine accumulation, the results showed that 3 treatments with relative light intensity of 75.02% ranked the top three.@*CONCLUSIONS@#In brief, the moderately strong light intensity and water content of medium from low to medium can facilitate the growth and yield of D. nobile plants, while light intensity from moderately weak to weak can enhance the dendrobine content.

Progress of effects of light on plant flavonoids / 中国中药杂志

As an important secondary metabolites of medicinal plant, flavonoids plays a very important role on itself including light protection and antioxidant. Light is one of the important environmental factors which impacts the secondary metabolites of plant and has a significant impact on biological synthesis of flavonoids. This paper reviews the recent progress of the effects of light on flavonoids on the plants focusing from light intensity and light quality, and summarizes briefly functions of the phenylalanine ammonia lyase (PAL) in plant secondary metabolism and the upstream key enzyme chalcone synthase (CHS) in flavonoid biosynthetic pathway.

Difference of the Nuclear Green Light Intensity between Papillary Carcinoma Cells Showing Clear Nuclei and Non-neoplastic Follicular Epithelia in Papillary Thyroid Carcinoma

BACKGROUND: There is subjective disagreement regarding nuclear clearing in papillary thyroid carcinoma. In this study, using digital instruments, we were able to quantify many ambiguous pathologic features and use numeric data to express our findings. METHODS: We examined 30 papillary thyroid carcinomas. For each case, we selected representative cancer cells showing clear nuclei and surrounding non-neoplastic follicular epithelial cells and evaluated objective values of green light intensity (GLI) for quantitative analysis of nuclear clearing in papillary thyroid carcinoma. RESULTS: From 16,274 GLI values from 600 cancer cell nuclei and 13,752 GLI values from 596 non-neoplastic follicular epithelial nuclei, we found a high correlation of 94.9% between GLI and clear nuclei. GLI between the cancer group showing clear nuclei and non-neoplastic follicular epithelia was statistically significant. The overall average level of GLI in the cancer group was over two times higher than the non-neoplastic group despite a wide range of GLI. On a polygonal line graph, there was a fluctuating unique difference between both the cancer and non-neoplastic groups in each patient, which was comparable to the microscopic findings. CONCLUSIONS: Nuclear GLI could be a useful factor for discriminating between carcinoma cells showing clear nuclei and non-neoplastic follicular epithelia in papillary thyroid carcinoma.

Research on development of automatic color LED medical cold light source / 中国医学装备

According to the clinical needs, to meet the different requirements of users,to provide a kind of energy-saving, environmental,Protection, safety,reliable automatic color LED medical cold light source; using LED as light source medical cold light source, LED used red, yellow, green The three primary colors, with the three drive control; light intensity using the total luminosity regulation combined with red, yellow, green three primary color Festival; automatic regulation, tracking and artificial regulation combined. According to the camera, display and individual different regulating the best color,taking LED as the light source, using the total light intensity and color separation light (red light, yellow light, green light intensity detection), and can realize thetracking, to develop the automatic tracking set color and intensity automatic colorLED medical cold light source. Conclusion automatic color LED medical cold light source of safe use, reliable performance, energy saving and environmental protection; the different needs can be suitable for different users; and can realize automatic control and manual adjustment of light intensity and light intensity of the total.

Cultivo de larvas de Ucides cordatus (LINNAEU, 1763) sob diferentes intensidades luminosas / Rearing of Ucides cordatus (LINNAEU, 1763) larvae under different light intensities

O caranguejo-uçá, Ucides cordatus, é uma espécie típica dos manguezais brasileiros e tem grande importância econômica para as populações litorâneas tradicionais. O presente trabalho investigou a influência da intensidade luminosa sobre a sobrevivência e a taxa de desenvolvimento larval de U. cordatus. Três intensidades luminosas foram avaliadas: claro - 710 lux, penumbra - 210 lux e escuro - 1 lux, em duas condições de cultivo, individual e coletivo. Houve diferenças significativas entre as taxas de sobrevivência das larvas zoea e as três intensidades luminosas avaliadas (p<0,05). As maiores taxas de ecdise para o estágio de megalopa foram obtidas no tratamento claro (42% nos cultivos coletivos e 30% nos cultivos individuais). No tratamento escuro, a metamorfose para megalopa foi de apenas 16% nos cultivos coletivos e de 7% nos cultivos individuais. Estes resultados indicam que a manutenção das larvas em baixas intensidades luminosas afeta negativamente a sobrevivência larval de U. cordatus...

Ucides cordatus is an edible crab species typical of Brazilian mangroves, and traditionally represents an important economic resource for many coastal populations. The present study investigated the influence of light intensity on the survival and rate of larval development of U. cordatus. Three different levels of luminosity were evaluated: 710 (Light), 210 Lux (Shaded) and 1 Lux (Dark), both in individual and collective cultivation conditions. Significant differences were found for survival of zoea larvae under the different light intensities (P<0.05). The greatest survival rates as well as rates of ecdysis to the megalopa stage were obtained under Light conditions (42% in collective cultures and 30% in individual cultures). In Dark conditions events of metamorphosis to megalopa stage was observed only in 16% of collective cultures and 7% of individual cultures. The result indicates that low light intensities may negatively affect larval survivorship during U. cordatus larval cultivations...

Photoperiodic effects on activity behaviour in the spiny eel (Macrognathus pancalus).

The study focused on the characteristics of circadian locomotor activity in the spiny eel, M. pancalus, kept under different photoperiodic conditions. Two experiments were conducted. Experiment 1 tested the light intensity dependent effect on circadian rhythmicity of the locomotor activity in spiny eel. Three groups of fish were entrained to 12L:12D conditions for 10 days. Thereafter, they were released to constant conditions for 15 days as indicated below: group 1-DD (0 lux), group 2- LLdim (~1 lux) and group 3-LLbright (~500 lux). The locomotor activity of the fish, housed singly in an aquarium, was recorded continuously with infrared sensors connected to a computer. More than 90% activity of the eels was confined to the dark hours suggesting nocturnal habit. Under constant conditions, the activity in 7/9 fish in group 1, 4/8 in group 2 and 3/8 in group 3, started free running with a mean circadian period of 24.48 ± 0.17 h, 23.21 ± 0.47 h and 25.54 ± 1.13 h in respective groups. Remaining fish in each group became arrhythmic. This suggests that spiny eel can be synchronised to LD cycle and under constant conditions they free run with a circadian period. However, their activity under LL is light intensity dependent; higher the intensity, more disruption in circadian locomotor activity. Experiment 2 was conducted to study the effect of decreasing night length (increasing photoperiod) on circadian locomotor activity. The fish were sequentially exposed to 16D (8L:16D), 12D (12L:12D), 8D (16L:8D), 4D (20L:4D) and 2D (22L:2D) for 10 days in each condition, thereafter, they were released in constant dark (DD= 0lux). The results showed that the duration of night length affects both, the amplitude and duration of locomotor activity. It can be concluded that the spiny eels are nocturnal and that their locomotor activity is under the circadian control and may be influenced by the photoperiod.

Effects of light intensity on circadian activity behaviour in the Indian weaverbird (Ploceus philippinus).

Circadian (locomotor activity/perching) behaviour of the weaverbird (Ploceus philippinus) under different light intensities was studied. Six groups of birds were subjected to 12L:12D (L = 1000 and 10 lux and D = 0.3 lux) for two weeks, and thereafter released into constant dim illumination (LLdim = 0.3 lux). After two weeks of LLdim, birds were given a 2 h light pulse of 1000 lux at circadian time (CT) 12, 17 and 20, and exposure of LLdim was continued for another two weeks and the activity pattern was monitored. As expected, all birds were entrained under 12L:12D showing dense-activity in the group that was placed under light phase of 1000 lux. Under LLdim birds exhibited circadian activity rhythms with periods longer or shorter than 24 h. Light pulse at CT 12 caused small delay shift in the activity phase, but a larger delay in phase shift occurred when the pulse was given at CT 17. A pulse at CT 20 caused small advanced phase shift. Thus, photoperiodic weaverbird appears to show circadian system regulated behaviour as seen by activity-rest pattern under programmed light cycles.