ABSTRACT
Objective: To study the chemical constituents in 95% ethanol aqueous extract of Chuanxiong Rhizoma. Methods: The compounds were separated and purified by repeated column chromatography on silica gel and high performance liquid chromatography, and their structures were determined by spectroscopic data analyses. Results: Forty-one compounds were obtained. Among them, 34 compounds were identified as xanthotoxin (1), 3-butylidenephthalide (2), Z-ligustilide (3), senkyunolide A (4), senkyunolide H (5), senkyunolide I (6), coniferyl alcohol (7), 3-propylanisole (8), epoxyligustilide (9), 5-hydroxy-3-butylene-phthalide (10), chuanxiongdiolide R1 (11), neocnidilide (12), Z'-3,8-dihydro-6.6',7.3'a-diligustilide (13), tokinolide B (14), ansaspirolide (15), bergaptol (16), senkyunolide D (17), 4-hydroxy-3-butyl-phthalide (18), 5-hydroxymethylfurfural (19), vanillin (20), levistolide A (21), trans-ferulic acid (22), 4-hydroxy-3-methoxypropiophenone (23), ethyl ferulate (24), falcarindiol (25), oplopandiol (26), senkyunolide E (27), stigmasterol (28), pregnenolone (29), isopimpinellin (30), β-sitosterol (31), dibutylphthalate (32), diisobutylphthalate (33), and coniferyl aldehyde (34), respectively. Conclusion: Compounds 1, 8, 16, 23, 26, and 30 are isolated from Chuanxiong Rhizoma for the first time.