ABSTRACT
Background: Lipase enzyme has wide application in industries, particularly food and detergent, but high production cost has always limited its use. Extensive studies are underway on production of high quality and low cost lipase enzyme in large amounts, for which microbial sources have been found to be the best. Aim: To estimate the potential of oil cakes for bacterial lipase production. Methodology: By-products of different oil seeds viz. neem, sesame, flax, mustard, coconut, castor, and groundnut were used for the preparation of fermentation media to culture lipolytic Pseudomonas aeruginosa. Optimization of growth condition was done with respect to different parameters such as fermentation time, nitrogen supplements, carbon additives, and lipid sources. Results: A good lipolytic P. aeruginosa JCM5962 (T) strain was isolated from soil of sugarcane field. Results of the study showed that coconut, sesame, neem, flax and mustard oilcakes induced good lipolytic activity from bacteria. Negligible lipase activity was obtained when organism was cultured in castor and groundnut oilcake medium. 1% ammonium nitrate as an additional nitrogen supplement was found to be ideal parameter for improved production. Conclusion: According to present work, lipases could be economically produced by P. aeruginosa using low cost oil cakes as potent substrate for fermentation medium.
ABSTRACT
Industrially important extracellular lipase enzyme production was explored by utilizing microbial strain isolated from dairy effluents. Alcaligenes viscosus DOGE-1 strain isolated from dairy waste waters proved to produce extracellular lipase. Various growth factors were attempted to maximize the lipase production by this strain. Growth factors like NH4PO4, Peptone, Urea coupled with peptone, KH2PO4, Olive oil and pH were found to be favored the maximum lipase production. This microbial strain was found to have a high lipolytic activity.
ABSTRACT
The effect of dark tea fermentation liquid with Eurotium Cristatum on the activity of digestive enzyme was researched, as fellowing amylase, protease, lipase. The results showed that dark tea fermentation liquid with Eurotium Cristatum may increase remarkably the activity of ?-amylase and protease, but decrease efficiently the activity of lipase. The fermentation liquid improves the digestion and absorption of starch and protein, but inhibits the decomposition and absorption of fat, so it can explain the mechanism of Fu-brick tea's health functions.