ABSTRACT
Objective:To isolate the chemical constituents from lipid-soluble parts of Tibetan Tinospora sinensis and identify the structure of the monomer compounds. Method:Eighty kg of dried rhizomes of T. sinensis were soaked by 80% ethanol (5 times of the dose of crude drug) for 1 hour, boiled under reflux for 1 hour,then and filtered. The above steps were repeated. The two extracts were combined,and the solvent was recovered to obtain a total extract. The obtained extract was extracted with a conventional solvent,and the solution was recovered to obtain petroleum ether extract,methylene chloride extract,ethyl acetate extract,n-butanol extract and water extract. Methylene chloride extract was isolated and purified by silicagel column chromatography,semi-preparative liquid chromatography and SephadexLH-20 chromatography; the chemical structure was identified on the basis of ESI-MS,nuclear magnetic resonance (1H-NMR and 13 C-NMR) spectroscopy data and literatures. Result:Fifteen compounds were isolated and identified respectively as n-dodecanol (1),n-hexacosanol (2),palmitic acid (3),dibutyl phthalate (4),vanillic acid (5),vanillin (6),apocynin (7),tinocordifolioside (8),medioresinol (9),isolariciresinol (10),aurantiamide (11),aurantiamide acetate (12),berberine (13),daucosterol (14),β-sitosterol (15). Conclusion:Except for 3,4,6,14,15,the other 10 compounds were isolated from the plant for the first time.
ABSTRACT
To study the fingerprints of liposoluble components in Salvia yunnanensis roots collected from different habi-tats, and compare with the fingerprints of Salvia miltiorrhiza. Methods: The fingerprints were determined by HPLC and the results were analyzed by a similarity evaluation system for chromatographic fingerprints of TCM ( Version 2004 A) . Results:The fingerprints of different samples of Salvia yunnanensis roots showed high similarity. And the characteristic peaks in the fingerprints of the liposoluble components were basically the same as those of Salvia miltiorrhiza, while the contents of several main effective components were lower than those in Salvia miltiorrhiza. Conclusion: The liposoluble components in Salvia yunnanensis roots show relatively high similarity with those in Salvia miltiorrhiza, however, there are obvious differences in the contents of the main constituents.
ABSTRACT
This study was aimed to establish the HPLC fingerprint of liposoluble components inDanshen capsule for quality evaluation. HPLC was run on a Welch ultimate XB-C18 column (250 mm × 4.6 mm, 5 μm) with acetonitrile-water as mobile phase in gradient elution at a flow rate of 1 mL·min-1. The column temperature was 25℃. The detection wavelength was 270 nm. The injection volume was 10μL. The results showed that 7 and 11 common peaks were selected as the HPLC fingerprint of liposoluble components inDanshen capsule from two manufactures, respectively. The similarities of 20 batches of samples were above 0.9. It was concluded that the analysis was stable and reproducible, which can be used as a basis for evaluating the quality control of liposoluble components in Danshen capsule.
ABSTRACT
Objective To obtain excellent cultivars of Salvia miltiorrhiza cultivated in Jiangsu Province by selective breeding and provide some basic reference for systemic breeding.Methods Tanshinones(tanzhinone Ⅱ_A and cryptotanshinone) and salvianolic acids(salvianolic acid A and B) in the roots of the four cultivars of S.miltiorrhiza were determined by HPLC.The total tanshinones and salvianolic acids were determined by spectrophotometry.Biomasses of four cultivars and correlative analysis between biomasses and effective components were analyzed with Excel and SPSS software.Results Accumulating of biomasses of the four cultivars was coincident incompletely.Contents of total tanshinone,tanshinone Ⅱ_A,cryptotanshinone,salvianolic acid B,danshensu,and total salviandic acids were different.Correlative analysis showed that the relativity between biomasses and contents of effective components of four cultivars were negative correlative.Relativity among liposoluble components was distinct.Correlation coefficients between tanshinone Ⅱ_A and cryptotanshinone of four cultivars were 0.943(ZY),0.942(DY),(0.715)(XY),and 0.960(ZC),respectively.Correlation coefficients between salvialic acid A and B of four cultivars were 0.766(ZY),0.416(DY),0.841(XY),and 0.618(ZC),respectively.But the relativity between hydrosoluble and liposoluble components was not obvious.Accumulating trends of liposoluble and hydrosoluble components were different.Conclusion Contents of total diterpenoids and tanshinon Ⅱ_A in little leaf cultivars are more than the contents of other three cultivars.The qualities of other three cultivars are better than the qualities of S. miltiorrhiza recorded in Chinese Pharmacopoeia.