ABSTRACT
To prepare a lipid nanoparticle (LNP)-based subunit vaccine of Mycobacterium tuberculosis (Mtb) antigen EsxV and study its immunological characteristics, the LNP containing EsxV and c-di-AMP (EsxV: C: L) was prepared by thin film dispersion method, and its encapsulation rate, LNP morphology, particle size, surface charge and polyphase dispersion index were measured. BALB/c mice were immunized with EsxV: C: L by nasal drops. The levels of serum and mucosal antibodies, transcription and secretion of cytokines in lung and spleen, and the proportion of T cell subsets were detected after immunization. EsxV: C: L LNPs were obtained with uniform size and they were spherical and negatively charged. Compared with EsxV: C immunization, EsxV: C: L mucosal inoculation induced increased sIgA level in respiratory tract mucosa. Levels of IL-2 secreted from spleen and ratios of memory T cells and tissue-resident T cells in mice were also elevated. In conclusion, EsxV: C: L could induce stronger mucosal immunity and memory T cell immune responses, which may provide better protection against Mtb infection.
Subject(s)
Animals , Mice , Mycobacterium tuberculosis , Antigens, Bacterial , Immunization , Nanoparticles , Vaccines, Subunit , Mice, Inbred BALB CABSTRACT
Objective: To investigate the effects of liposome nanoparticles of hydrazinocurcumin (HC-NPs) on cells proliferation, apoptosis, invasion, and migration in human breast cancer MDA-MB-231 cells. Methods: Liposome HC-NPs were prepared by film-sonication method and then were used to treat human breast cancer MDA-MB-231 cells. The treated cell survival was analyzed by MTT; Morphological changes were detected by Liu staining; The cell cycle and apoptosis were investigated by FCM assay; Then invasion and migration of the cells were analyzed by Transwell. Moreover, the expression of proteins correlated to cell cycle arrest, apoptosis, invasion, and migration were detected by Western blotting. Results: The cell proliferation was inhibited by HC-NPs, the cells became round in shape, and the cells were arrested in G 2/M phase, the ratio of apoptosis was increased (P<0.01), the cells invasion and migration were restrained. The expression of p-STAT3, CyclinD1, Survivin, Bcl-2, and MMP-9 were down-regulated, while the expression of Bax was up-regulated. Conclusion: The effects of HC-NPs on the suppression of cell proliferation, invasion, and migration, and induction of cells apoptosis may be related to the inhibition of STAT3 activation.