ABSTRACT
BACKGROUND: Adipose stem cell-free liquid extracts include adipose stem cell conditioned medium, adipose stem cell exosomes, because they do not contain cells and have the advantages of being easy to carry, store, and transport, which has gradually become one of the most promising therapies currently. OBJECTIVE: To review the research progress in the therapeutic application of adipose stem cell-free liquid extracts. METHODS: The first author searched the CNKI, Wanfang and PubMed databases for relevant articles published from January 2005 to March 2020. The key words were “adipose stem cell, stromal cell and conditioned medium”, “adipose stem cell, stromal cell and exosomes” in Chinese, and “adipose stem cell, adipose stromal cell and conditioned medium” and “adipose stem cell, adipose stromal cell and exosomes” in English. Repetitive articles and those lacking of originality were eliminated. Totally 123 articles were searched initially, and 62 articles were included in result analysis. RESULTS AND CONCLUSION: Adipose stem cell-free liquid extracts have broad therapeutic potential in a variety of disease areas, such as anti-aging, wound healing, scar recovery, and nerve regeneration. However, the specific mechanism of action is not very clear, and further research is needed.
ABSTRACT
Objective To develop a stable method for fingerprint analysis of extractum platycodi liquidum to ensure its u‐niformity and stability .Methods UPLC‐ELSD was applied to obtain a chromatogram with 11 well separated peaks ,and the chromatographic condition was as follows:mobile phase was gradient elution by acetonitrile‐water ,column was ACQUITY UPLC BEH C18 (2 .1 × 100 mm ,1 .7 μm) with the column temperature of 30 ℃ and the flow rate of 0 .2 ml/min .Results The chromatographic fingerprints were analyzed with similarity evaluation system for chromatographic fingerprint of TCM .Similar‐ities were all greater than 0 .90 ,which could meet the requirement for chromatographic fingerprint of TCM .Conclusion The stability ,repeatability and precision of the developed method were all validated ,and the chromatographic fingerprint analysis was a rapid and accurate method for the quality evaluation of traditional Chinese medicine .
ABSTRACT
Objective Effects of aqueous extract from Lishi No.5 formula on concentration of intracellular free-calcium fluorescent intensity in neurons. Methods Hippocampus neurons of 1-day newborn SD rats were cultured with conventional culture technique. The cells cultured for 9-12 days were used for experiment. Intracellular free calcium fluorescent intensity of neurons cultured under different conditions was assayed with confocal microscopic calcium image technique after loading of Fluo-3/AM. Results Free-calcium concentration was enhanced by aqueous extract from Lishi No.5 formula, this concentration was up to 126.35?9.35nmol/L, but the concentration is at normal scope; L-type calcium channel blocker Nifedipine may block the effect of aqueous extract from Lishi No.5 formula enhancing intracellular free-calcium concentration in part, it make intracellular free-calcium concentration down to 90.75?10.15nmol/L, but Nifedipine itself also decreased markedly free-calcium concentration to 40.65?5.65nmol/L. NMDA increase Markedly calcium fluorescent intensity, the effects of NMDA was decreased notably after pre-treating with aqueous extract from Lishi No.5 formula. The effect of MK-801, an antagonist of NMDA receptor, on inhibition of NMDA increasing free calcium fluorescent intensity was significantly reduced after pretreatment of aqueous extract from Lishi No.5 formula. Conclusion Aqueous extract from Lishi No.5 formula maybe bidirectional adjust intracellular free calcium concentration via enhancing L-type calcium channel activity and blocking NMDA receptor in part.