ABSTRACT
Twelve compounds were isolated from Liquidambaris Resina by silica gel column chromatography and thin layer chromatography. Their structures were identified on the basis of spectral data, electron capture detector data, and physicochemical properties as(2'R, 3'R)-2',3'-dihydroxy-hydrocinnamyl-(E)-cinnamate(1),(E)-cinnamyl-(E)-cinnamate(2), cinnamic acid(3), 28-norlup-20(29)-en-3-one-17β-hydroperoxide(4), erythrodiol(5), 13β,28-epoxy-30-hydroxyolean-1-en-3-one(6),(3β)-olean-12-ene-3,23-diol(7), 2α,3α-dihydroxy-olean-12-en-28-oic acid(8), 28-hydroxyolean-12-en-3-one(9), 3-epi-oleanolic acid(10), 3-oxo-oleanolic acid(11), and hederagenin(12). Compound 1 was a new cinnamic acid ester derivative and compounds 2-4,6-8, and 12 were isolated from Liquidambaris Resina for the first time. Compounds 4, 5, 10, and 12 exerted inhibitory effects on the proliferation of human umbilical vein endothelial cells(HUVEC) with the IC_(50) values of(17.43±2.17),(35.32±0.61),(27.50±0.80), and(46.30±0.30) μmol·L~(-1), respectively.
Subject(s)
Humans , Oleanolic Acid , Endothelial Cells , Esters , Cinnamates , Triterpenes/chemistry , Molecular StructureABSTRACT
Objective: To study the chemical constituents from Liquidambaris Fructus (fruits of Liquidambar formosana). Methods: The constituents were isolated and purified by preparative TLC and silica gel, Sephadex LH-20, and ODS column chromatography, and their structures were elucidated on the basis of their physicochemical properties and spectral data. Results: Seven compounds were obtained from 70% ethanol extract of the fruits of Liquidambaris Fructus and identified as 11α-methoxyl-28-nor-β-amyrenone (1), 28-nor-β-amyrenone (2), 3-oxo-12α-hydroxy-oleanan-28, 13β-olide (3), 3α-acetoxyl-25-hydroxy-olean-12-en-28-oic acid (4), erythodiol (5), betulonic acid (6), and styracin (7), respectively. Conclusion: Compound 1 is a new triterpenoid named liquidaformone A, and compounds 5 and 7 are isolated from the plants of Liquidambar Linn. for the first time.
ABSTRACT
Some Xylaria materials growing on the fruits of Liquidambar spp. were collected. They were identified as X. persicaria on the basis of morphological characteristics and sequence analysis of the complete ITS region (ITS1-5.8S-ITS2) of rDNA. This is the first record of this species from Korea.