ABSTRACT
Objective To observe the effect of low concentration Aβ1-42 monomer/oligomers and CORM-2 in different concentration on livability of SN56 cells. Methods SN56 cells were cultured in the 96-well plate with uniform concentration, and were divided into control group, Aβ1-42 group, Aβ1-42 + CORM-2 50μM group, and Aβ1-42 + CORM-2 100 μM group. Three lines of cells in Aβ1-42 group were cultured in the surroundings of 10nM,100nM and 1 μM Aβ1-42monomer/oligomers, respectively. Aβ1-42 + CORM-2 50μM group and Aβ1-42 + CORM-2 100μM group had the same culture condition as group Aβ1-42 ,except contain 50μM, and 100μM CORM-2, respectively. Control group didnt have any effect factor. Three days later,the livability of different groups was compared with MTT method. Results The livability of group Aβ1-42 with the increasing concentration of Aβ1-42 was (79.73 ±0.94)% ,(67.99 ±0.79)% ,(60.42 ±0.62)% , respectively. The higher the concentration of Aβ1-42 was,the lower the livability of SN56 cell was. The livability of group Aβ1-42 + CORM-2 50μM/100μM was( 75.15±0.096)%,(63.20 ±0.17)%, (55.33 ±0.19)%; (73.20 ±0.27)%, (64.34 ±0.11 )%, (54.17 ±0.12)% , respectively. Both were lower than group Aβ1-42. And different CORM-2 concentration had discrepancy in the ability of decreasing the cell livability. Conclusion Low concentration of Aβ1-42 can reduce the livability of SN56 cells, and higher concentration has more significant effect; CORM-2 in different concentration both can decrease the livability of SN56 cells,and there is a discrepancy in the intensity.
ABSTRACT
Para matrizes avícolas tipo corte o manejo de alimentação é de extrema importância, principalmente durante a fase de recria, período no qual a ave está desenvolvendo sua estrutura corporal. A resposta em termos de produção destas aves, depende da maneira como foram recriadas. Com o objetivo de avaliar diferentes programas de arraçoamento na recria de matrizes avícolas tipo corte, sobre o peso corporal, uniformidade de peso corporal e viabilidade criatória, conduziu-se este experimento no Setor de Avicultura do Departamento de Zootecnia da UFSM. O delineamento experimental foi inteiramente casualizado, constituído de 3 tratamentos com 4 repetições, tendo cada unidade experimental 67 aves. Na 1ª e 2ª semana de idade as aves foram alimentadas à vontade, na 3ª e 4ª semanas o arraçoamento foi diário controlado. Durante a fase de recria (5ª a 20ª semana de idade), as aves foram submetidas aos tratamentos (programas de arraçoamento): T1: 5ª a 14ª semana = dia sim, dia não ("Skip-a-Day"); 15ª a 17ª = 2 dias sim/1 dia não e da 18ª a 20ª semana, arraçoamento diário controlado; T2: 5ª a 8ª semana = dia sim, dia não ("Skip-a-Day"), 9ª a 16ª = método 48-57-63h e da 17ª a 20ª semana = 2 dias sim/1 dia não; T3: 5ª a 20ª semana de idade = 48-57-63h de intervalo entre arraçoamentos. Os resultados obtidos mostraram que, pode-se recriar matrizes com maior intervalo entre arraçoamentos (48-57-63 horas), sem haver comprometimento dos parâmetros avaliados neste trabalho, e ainda com a vantagem de não ser necessário o arraçoamento nos finais de semana, reduzindo os custos com mão de obra.
Feeding management is estremely important for broiler breeders, mainly during the rearing period, when the birds develop their corporal structure. The production performance of the hens depends on the conditions they were grown. An experiment was done at the Poultry Section of the Animal Science Department of the Federal University of Santa Maria, to study the of feeding programs on the body weight gain, uniformity of weight and viability of broiler breeders. A completely randomized designe with 3 treatments and 4 replicates of 67 birds was used. In the fírst 2 weeks of age they were all feed ad libitum, and in the 2 subsequent weeks feed was controled daily. From 5 to 20 weeks they were submitted to the following feeding treatments: T1: 5-14 weeks = "Skip-a-Day", 15-17 = "Skip on day out of two and 18-20 = controled daily; T2: 5-8 weeks = "Skip-a-Day", 9-16 = 48-57-63 hours of feeding intervals and 17-20 = ''Skip one day out of two"; T3 = 5-20 " 48-57-63 hours intervals between feeding. The results show that the feeding program of 48-57-63 hours interval between feeding during the rearing period can be used with no negative effects on the parameters studied, and with the advantage ofno need of feeding during the weekend, saving handwork costs.