Spontaneous poisoning by Dodonaea viscosa (Sapindaceae) in cattle in Southern Brazil / Intoxicação espontânea por Dodonaea viscosa (Sapindaceae) em bovinos no sul do Brasil

In this study, an outbreak of spontaneous poisoning by Dodonaea viscosa (D. viscosa) in a herd of dairy cattle in the municipality of Capão do Leão, Rio Grande do Sul, was investigated. Three deaths occurred in a batch of 16 Jersey cattle, aged between three and four years, kept in a native field. The clinical signs observed were apathy, decreased production, and anorexia, with death occurring within approximately 48 h after the onset of signs. The three cattle were necropsied, and tissue samples were sent for histopathological examination. Necropsy findings included serosanguineous fluid in the abdominal cavity, intestines with congested serosa, and marked mesenteric edema. The mucosa of the abomasum of two of the animals was hemorrhagic with bloody content, and among the ruminal content of a bovine, leaves with morphological characteristics compatible with D. viscosa were observed. The livers of the three animals were enlarged, with accentuation of the lobular pattern. Histologically, centrilobular coagulation necrosis with congestion and hemorrhage was observed in the liver. Vacuolization and degeneration of hepatocytes were observed in the mid-zonal and periportal regions. The diagnosis of poisoning by D. viscosa leaves was based on epidemiological data, necropsy findings, and histopathological alterations. The presence of the plant in the rumen and in the grazing site of the affected cattle was essential for the diagnosis.(AU)

Neste trabalho, é descrito um surto de intoxicação espontânea por Dodonaea viscosa (D. viscosa) ocorrido em um rebanho de bovinos leiteiros, no município de Capão do Leão, no Rio Grande do Sul. Ocorreram três mortes em um lote de 16 bovinos da raça Jersey com idades entre três e quatro anos, mantidos em campo nativo. Os sinais clínicos observados foram apatia, queda na produção e anorexia, com morte em aproximadamente 48 horas após o início dos sinais. Os três bovinos foram necropsiados, e amostras de tecidos foram encaminhadas para exame histopatológico. Os achados de necropsia incluíam líquido serossanguinolento na cavidade abdominal, intestinos com serosas congestas e marcado edema de mesentério. A mucosa do abomaso de dois animais apresentava-se hemorrágica com conteúdo sanguinolento e, em meio ao conteúdo ruminal de um bovino foram observadas folhas com caracteres morfológicos compatíveis com D. viscosa. O fígado dos três animais estava aumentado, com acentuação do padrão lobular. Histologicamente no fígado havia necrose de coagulação centrolobular com congestão e hemorragia. Nas regiões médio-zonal e periportal observou-se vacuolização e degeneração dos hepatócitos. O diagnóstico de intoxicação pelas folhas D. viscosa foi baseado nos dados epidemiológicos, nos achados de necropsia e nas alterações histopatológicas. A presença da planta no rúmen e no local de pastoreio dos bovinos afetados foi fundamental para o diagnóstico.(AU)

Spontaneous poisoning by Dodonaea viscosa (Sapindaceae) in cattle in Southern Brazil

ABSTRACT: In this study, an outbreak of spontaneous poisoning by Dodonaea viscosa (D. viscosa) in a herd of dairy cattle in the municipality of Capão do Leão, Rio Grande do Sul, was investigated. Three deaths occurred in a batch of 16 Jersey cattle, aged between three and four years, kept in a native field. The clinical signs observed were apathy, decreased production, and anorexia, with death occurring within approximately 48 h after the onset of signs. The three cattle were necropsied, and tissue samples were sent for histopathological examination. Necropsy findings included serosanguineous fluid in the abdominal cavity, intestines with congested serosa, and marked mesenteric edema. The mucosa of the abomasum of two of the animals was hemorrhagic with bloody content, and among the ruminal content of a bovine, leaves with morphological characteristics compatible with D. viscosa were observed. The livers of the three animals were enlarged, with accentuation of the lobular pattern. Histologically, centrilobular coagulation necrosis with congestion and hemorrhage was observed in the liver. Vacuolization and degeneration of hepatocytes were observed in the mid-zonal and periportal regions. The diagnosis of poisoning by D. viscosa leaves was based on epidemiological data, necropsy findings, and histopathological alterations. The presence of the plant in the rumen and in the grazing site of the affected cattle was essential for the diagnosis.

RESUMO: Neste trabalho, é descrito um surto de intoxicação espontânea por Dodonaea viscosa (D. viscosa) ocorrido em um rebanho de bovinos leiteiros, no município de Capão do Leão, no Rio Grande do Sul. Ocorreram três mortes em um lote de 16 bovinos da raça Jersey com idades entre três e quatro anos, mantidos em campo nativo. Os sinais clínicos observados foram apatia, queda na produção e anorexia, com morte em aproximadamente 48 horas após o início dos sinais. Os três bovinos foram necropsiados, e amostras de tecidos foram encaminhadas para exame histopatológico. Os achados de necropsia incluíam líquido serossanguinolento na cavidade abdominal, intestinos com serosas congestas e marcado edema de mesentério. A mucosa do abomaso de dois animais apresentava-se hemorrágica com conteúdo sanguinolento e, em meio ao conteúdo ruminal de um bovino foram observadas folhas com caracteres morfológicos compatíveis com D. viscosa. O fígado dos três animais estava aumentado, com acentuação do padrão lobular. Histologicamente no fígado havia necrose de coagulação centrolobular com congestão e hemorragia. Nas regiões médio-zonal e periportal observou-se vacuolização e degeneração dos hepatócitos. O diagnóstico de intoxicação pelas folhas D. viscosa foi baseado nos dados epidemiológicos, nos achados de necropsia e nas alterações histopatológicas. A presença da planta no rúmen e no local de pastoreio dos bovinos afetados foi fundamental para o diagnóstico.

Intoxicação experimental por Trema micrantha (Cannabaceae) em equinos / Experimental Trema micrantha (Cannabaceae) poisoning in horses

O objetivo desse estudo foi confirmar a toxidez e caracterizar os aspectos clínicos e patológicos da intoxicação por Trema micrantha em equinos. Três equinos, pôneis, com idade entre 2 e 7 anos consumiram espontaneamente folhas de T. micrantha em doses únicas de 30g/kg, 25g/ kg e 20g/kg. Os três animais adoeceram e evoluíram para morte. Outro equino recebeu 15 e 25g/kg da planta com intervalo de 30 dias entre as doses e não apresentou alteração clínica. Coletas diárias de sangue foram realizadas para análises bioquímicas. Os principais sinais clínicos apresentados foram apatia, desequilíbrios, dificuldade de deglutição, decúbito esternal, decúbito lateral, movimentos de pedalagem, coma e morte. Os três equinos afetados apresentaram elevação da atividade sérica de gama-glutamil transferase, dos níveis séricos de amônia e diminuição da glicemia. Esses animais foram necropsiados e fragmentos de diversos órgãos foram coletados para análise histopatológica e imuno-histoquímica. Os principais achados patológicos foram encontrados no fígado e no encéfalo dos três animais. O fígado apresentava, macroscopicamente, acentuação do padrão lobular; enquanto que, no encéfalo havia áreas amareladas na superfície de corte, mais evidentes na substância branca do cerebelo. Microscopicamente, o fígado apresentava tumefação hepatocelular, necrose de coagulação predominantemente centrolobular e hemorragia associada. No encéfalo, havia edema perivascular generalizado e astrócitos Alzheimer tipo II na substância cinzenta. Esses astrócitos apresentaram marcação fraca ou negativa na imuno-histoquímica anti-GFAP e marcação positiva do antígeno S-100. A dose letal mínima de folhas de T. micrantha estabelecida nesse experimento foi de 20g/kg. A ampla distribuição e palatabilidade desta planta, associadas à alta sensibilidade da espécie equina, constatada nesse experimento, reforçam a importância da planta em casos acidentais de intoxicação em equinos.

The aim of this study was to confirm the toxicity and characterize the clinical and pathological aspects of poisoning by Trema micrantha in horses. The green leaves of the plant were spontaneously consumed by three ponies at single doses of 20, 25, and 30g/kg. The three ponies developed clinical disease characterized by apathy, equilibrium deficit, deglutition difficulty, sternal or lateral recumbency, paddling, coma and death. Affected ponies had also enhanced seric activity of gamma-glutamyl transferase, seric ammonia apart of diminished glycemia. An additional pony, that received 15 and 25g/kg of T. micrantha with a 30 day interval between the two doses, showed no clinical disease. The three ponies were necropsied and fragments from organs were collected for histology and immunostaining (IS) procedures. The main pathological findings were in the liver and encephalon. There were enhanced lobular pattern of the liver and yellowish areas in the cut surface of the encephalon, especially visualized in the cerebellar white matter. Microscopically, there was hepatocellular swelling and coagulative hepatic necrosis predominantly centrilobular with hemorrhages. Generalized perivascular edema and Alzheimer type II astrocytes were observed in the encephalon. The Alzheimer type II astrocytes showed weak or absent anti-glial fibrillar acid protein IS associated with positive IS for S-100 protein. The minimal lethal dose of Trema micrantha leaves was established at 20g/kg. The sensibility of horses to this plant, its wide distribution and high palatability reinforces the importance of Trema micrantha in accidental poisoning of horses.

Intoxicação espontânea por larvas de Perreyia flavipes (Pergidae) em suínos no estado de Santa Catarina / Spontaneous poisoning by larvae of Perreyia flavipes (Pergidae) in pigs in the state of Santa Catarina

Descreve-se um surto de intoxicação espontânea por Perreyia flavipes em suínos. O surto ocorreu no final de maio de 2009, na cidade de Urubici, planalto serrano do Estado de Santa Catarina, Brasil. A propriedade tinha aproximadamente 50 animais criados extensivamente e desses 10 animais adoeceram. Esses suínos apresentavam anorexia, apatia, movimento constante de cabeça e bater de orelhas, dificuldade de caminhar, cambaleio, ranger de dentes e a agitação aumentava mediante ruídos e movimentos próximos. Na necropsia as alterações observadas foram a marcada evidenciação do padrão lobular hepático e a presença de larvas de P. flavipes misturadas ao conteúdo estomacal. Microscopicamente observou-se necrose de coagulação dos hepatócitos, com distribuição centrolobular a massiva que era acompanhada de congestão e hemorragia acentuada, restando uma ou duas camadas de hepatócitos com degeneração vacuolar na região portal. Os aspectos clínicos, epidemiológicos e as lesões caracterizaram hepatite tóxica por larvas de P. flavipes em suínos.

The study reports an outbreak of spontaneous poisoning by Perreyia flavipes in pigs. The outbreak occurred at the end of May 2009, in the municipality of Urubici, plateau of Santa Catarina, Brazil. The farm had about 50 pigs reared extensively and 10 animals got sick. The clinical signs were anorexia, apathy, constant movement of the head and hitting the ears, difficulty to walk and stagger, gnashing of teeth and agitation that increased with noise and movement nearby. At necropsy, pronounced hepatic lobular pattern and P. flavipes larvae mixed with the stomach content were observed. Microscopically, hepatocellular centrilobular to diffuse coagulation necrosis with severe congestion and hemorrhage was observed, with vacuolar degeneration in one or two layers of hepatocytes in the portal zones. Clinical signs, epidemiology and lesions in the pigs were characteristic of toxic hepatitis by larvae of P. flavipes.

Intoxicação por indigofera suffruticosa (Leg. Papilionoideae) em bovinos / Poisoning by indigofera suffruticosa (Leg. Papilionoideae) in cattle

Em uma propriedade do Município de Aparecida, no sertão da Paraíba, foi diagnosticada intoxicação por Indigofera suffruticosa em um rebanho de 25 vacas e um boi que foram colocados em um piquete que continha predominantemente I. suffruticosa onde permaneceram durante 10 dias. No quinto dia de pastejo o proprietário observou urina com coloração vermelho escura em uma vaca e ao final de dez dias de pastejo havia seis vacas doentes apresentando hemoglobinúria e diminuição na produção leiteira. No quinto dia após serem retiradas do pasto uma vaca apresentou agressividade e no sétimo dia foi encontrada morta pela manhã. Na necropsia o fígado apresentava coloração amarelada com pontos avermelhados e aumento do padrão lobular. A bexiga encontrava- se repleta com urina de cor vermelho escura. Os rins estavam escuros e com áreas hemorrágicas, de até 2mm, que se estendiam radialmente para dentro do córtex e parte da medula. Na histologia, os rins apresentavam áreas multifocais de necrose tubular isquêmica aguda com deposição de hemoglobina nas células epiteliais e cilindros de hemoglobina nos túbulos. No fígado havia necrose de coagulação difusa paracentral e ocasionalmente centrolobular. Os demais bovinos afetados se recuperaram espontaneamente 3-8 dias após serem retirados da pastagem. Conclui-se que a intoxicação por I. suffruticosa apesar de apresentar recuperação espontânea na maioria dos bovinos, mesmo se estes continuam ingerindo a planta, pode causar a morte de alguns animais por anemia aguda.

Poisoning by Indigofera suffruticosa was diagnosed on a farm in the state of Paraíba, in the Brazilian semiarid region, in a herd of 25 cows and one bull. The herd was grazing for ten days in a paddock severely invaded by I. suffruticosa. Five days after the introduction into the paddock some cows showed red urine, and in ten days, when the herd was removed from the pastures, six cows had decreased milk production and hemoglobinuria. Five days after being withdrawn from the pastures, one cow showed aggressiveness and two days later was found death. At necropsy, the liver was yellowish with petechial hemorrhages on the surface and had increased lobular pattern. The kidneys were dark and with red spots up to 2mm in diameter in the surface, which extended radially into the renal cortex. The urinary bladder was full of dark red urine. Upon histologic examination the kidneys had multifocal areas of ischemic tubular necrosis with hemoglobin deposition in the epithelial cells and hemoglobin casts in the tubules. The liver had diffuse paracentral and occasionally centrolobular coagulative necrosis. The other affected cows recovered spontaneously within 3-8 days after having been moved from the paddock. It is concluded that poisoning by I. suffruticosa, despite the spontaneous recovering of most cattle, can cause the death of some affected animals by acute hemolytic anemia.

Intoxicação espontânea pelas larvas de Perreyia flavipes em bovinos no Estado de Santa Catarina, Brasil / Spontaneous poisoning by larvae of Perreyia flavipes in cattle from the State of Santa Catarina, Brazil

Neste trabalho, é descrito um surto de intoxicação por Perreyia flavipes ocorrido em agosto de 2006, em um rebanho bovino de 280 animais, no município de Sombrio, em Santa Catarina. Ocorreram 17 mortes em um lote de 77 bovinos de raça mista com idades entre um e dois anos mantidos em um piquete (P1) de aproximadamente 90 hectares por cerca de 30 dias. Após as primeiras duas mortes, os animais foram movidos para um segundo piquete (P2), onde ainda ocorreram 15 mortes nos cinco dias após a transferência. Seis bovinos foram necropsiados, e amostras de tecidos foram avaliadas para exame histológico. Os achados de necropsia incluíam hemorragias subcutâneas na mucosa do abomaso e superfície subepicárdica. Ascite e edema do abomaso, duodeno, pâncreas e vesícula biliar foram observados. O fígado apresentava-se amarelado com petéquias subcapsulares e acentuação do padrão lobular. No rúmen de três bovinos, foram encontrados fragmentos de corpos e cabeças de larvas de P. flavipes em meio ao conteúdo ruminal. Histologicamente o fígado apresentava tumefação hepatocelular e necrose centrolobular e mediozonal, difusa e acentuada, com hemorragia e congestão centrolobular. Havia depleção e necrose linfóide no baço, placas de Peyer e linfonodos mesentéricos. O diagnóstico de intoxicação pelas larvas da P. flavipes foi baseado nos dados epidemiológicos, nos achados de necropsia e nas alterações histopatológicas. As lesões hepáticas e em tecidos linfóides, além da grande quantidade de larvas de P. flavipes no primeiro piquete (P1) e no rúmen de animais necropsiados, foram dados importantes para o diagnóstico.

This article describes an outbreak of Perreyia flavipes poisoning in August of 2006 in a cattle herd of 280 animals in Sombrio, Santa Catarina, southern Brazil. Seventeen bovines between 1 and 2 years old from one herd of 77 mixed breed cattle died. The herd was kept in a paddock of 90 hectares (P1) for 30 days. After de first two deaths occurred, the herd was transferred to a second paddock (P2), and 15 more animals died in the next 5 days. Samples from 6 of these animals were analyzed. During the necropsy, hemorrhage was seen in the subcutaneous tissue, abomasal mucosa, and subepicardial area. The animals presented ascites and edema of the abomasal folds, duodenum, pancreas, and gallbladder. The liver was yellowish, had subcapsular petechial hemorrhages, and accentuated lobular pattern. P. flavipes larval body fragments and heads were found in the rumen content of three of the animals. In the histological examination the liver showed hepatocellular tumefaction, diffuse coagulative necrosis of centrilobular and midzone hepatocytes, hemorrhage and centrilobular congestion. Lymphoid depletion and diffuse necrosis of germinative centers of lymphoid follicles in mesenteric lymph nodes, Peyer’s patches and spleen were also observed. The diagnosis of intoxication by P. flavipes larvae in this outbreak was based on the epidemiological data, necropsy, and histological findings. The confirmation of P. flavipes larvae as cause of the outbreak was done by the observation of intact larvae and remnants in the ruminal content of the necropsied bovines and by the presence of a great number of larvae clusters in the paddock 1 (P1).

Intoxicação natural por Cestrum intermedium em bovinos no Rio Grande do Sul, Brasil / Spontaneous poisoning by Cestrum intermedium in cattle in Rio Grande do Sul, Brazil

Em junho de 2007, uma mortalidade de bovinos com sinais clínicos e lesões de insuficiência hepática aguda em uma propriedade, na região da encosta da serra do Rio Grande do Sul, foi atribuída ao consumo de Cestrum intermedium. De um total de 17 bovinos, dois morreram com curso clínico inferior a 12 horas, apresentando tremores musculares, andar cambaleante, reações agressivas, decúbito lateral e morte. À necropsia, o fígado estava moderadamente aumentado de volume e com acentuação do padrão lobular. Histologicamente, o fígado apresentava necrose de coagulação centrolobular, difusa, aguda e acentuada. As alterações patológicas associadas à presença e a evidência de consumo da planta pelos bovinos permitiram o diagnóstico.

In June of 2007, mortality in cattle with clinical signs and lesions of acute hepatic insufficiency on a farm located in the hillside of Rio Grande do Sul mountain range was attributed to Cestrum intermedium consumption. From 17 bovines, two died up to 12 hours presenting clinical signs such as muscular tremors, incoordination, aggressiveness, lateral recumbence and death. Macroscopic lesions were found primary in the liver and were characterized by a slightly increased volume and diffuse accentuation of the lobules. Microscopically, the liver showed diffuse and accentuated centrilobular coagulative necrosis. The presence of Cestrum intermedium plants that was being ingested by the bovine associated with the hepatic lesions was decisive for the diagnosis.

Spontaneous poisoning by larvae of Perreyia flavipes (Pergidae) in sheep

From a flock of 175 Texel sheep 25 animals died after consumption of a sawfly larvae subsequently identified as Perreyia flavipes. The disease occurred in June-July 2006 on a farm located in the county of Encruzilhada do Sul, Rio Grande do Sul, Brazil. Although there were 11 cattle in the same paddock, none of them was affected. High numbers of compact masses containing up to 150 larvae were scattered in the paddock where the animals were grazing. Most affected sheep showed severe apathy during 24-36 h before death, but weakness, muscular tremors and depression were also observed. Necropsy was performed on six sheep and the main macroscopic lesions were hemorrhages in the subcutaneous tissues, endocardium, gallbladder wall, and abomasal mucosa. In all animals was found hydrothorax, hydropericardium, ascites, and mild jaundice. Edema in the abomasal folds, mesentery, perirenal tissues, and gallbladder wall were also seen. The livers were yellowish with disseminated pinpoint hemorrhages in the parenchyma and had an enhanced lobular pattern. Perreyia flavipes larval body fragments and heads were found in the forestomach contents of the six sheep. Feces were scant, dry and formed balls coated by mucus and streaks of blood. Similar contents were also present at the end of the cecum. Prominent microscopic lesions included severe and diffuse periacinar or massive necrosis of hepatocytes associated with multifocal random hemorrhages. Diffuse necrosis of lymphoid follicles in lymph nodes and Peyer's patches, lymphoid depletion and necrosis in germinative centers of the spleen, and diffuse vacuolization in the renal tubular epithelia were also seen.

Vinte e cinco ovinos Texel de um rebanho de 175 morreram após ingerirem larvas posteriormente identificadas como Perreyia flavipes. A doença ocorreu em Junho e Julho de 2006, em uma propriedade localizada em Encruzilhada do Sul, Rio Grande do Sul. Embora houvesse também 11 bovinos no mesmo potreiro, nenhum foi afetado. Grandes quantidades de massas compactas, contendo cada uma mais de 150 larvas, estavam espalhadas na pastagem onde os animais pastoreavam. Os ovinos afetados apresentavam apatia severa por 24 a 36 horas antes da morte. Fraqueza, tremores musculares e depressão também foram vistos. Em necropsias realizadas em seis animais, as principais alterações macroscópicas foram hemorragias no tecido subcutâneo, endocárdio, parede da vesícula biliar e mucosa abomasal. Hidrotórax, hidropericárdio, ascite e icterícia estavam presentes em todos os animais. Edema nas pregas do abomaso, mesentério, tecidos peri-renais e parede da vesícula biliar também foram observados. Os fígados estavam amarelados, com petéquias disseminadas no parênquima e padrão lobular aumentado. Fragmentos de corpos e cabeças de larvas de Perreyia flavipes foram encontrados nos conteúdos ruminais dos seis animais. O conteúdo da porção final do cólon, ceco e reto estava seco, com muco aderido e estrias de sangue. Necrose de hepatócitos periacinar ou massiva associada com hemorragias multifocais aleatórias foi o principal achado histológico, mas também havia necrose difusa em folículos linfóides nas placas de Peyer e linfonodos, depleção linfóide e necrose em centros germinativos no baço e vacuolização no epitélio tubular renal.

Hepatocellular Carcinoma with Internal Extensive Coagulation Necrosis: Carefulness of Preoperative Imaging Diagnosis and Comparison with Surgical Specimen

PURPOSE: The aim of this study is to correlate the non-characteristic dual-phase CT imaging findings of hepato-cellular carcinoma with the observed characteristics of surgical specimens. MATERIALS AND METHODS: We studied four cases in which homogeneous low attenuation was observed during the arterial and delayed phases of dynamic CT scanning and in which hepatocellular carcinoma with coagulation necrosis above 95% was pathologically confirmed. We compared the findings of dual phase CT scanning, ultrasonography, angiography and Lipiodol CT scanning with the observed features of surgical specimens. RESULTS: Nodules were 30-50 (mean, 41) mm in size, and were round in three cases and oval in one. In all four cases, a low density lesion was observed during the arterial and delayed phases of dual-phase CT scanning. Ultrasonography demonstrated internal echo and the presence of a hypoechoic halo, implying that in all cases a capsule was present. At angiography and LiCT, minimal peripheral and central tumor staining or lipiodol up-take was observed. In all surgical specimens a complete capsule was visible, and histologic structures were mainly of the trabecular type, Edmondson grade II or III was recorded, and the mass had undergone extensive coagulation necrosis (above 95%). CONCLUSION: In cirrhotic liver which is hepatitis B-antigen positive, clear sonographic findings of internal echo and a capsule, rather than a simple cyst, indicate the possibility of hepatocellular carcinoma with extensive coagulation necrosis. This is so even if the arterial and delayed phases of dual-phase CT scanning indicate the presence of a low-density lesion, and in such cases additional work-up is therefore required.

Effects of human fetal hepatic stimulator substance on D-galac-tosamine-induced liver necrosis in rats / 第三军医大学学报

The effects of human fetal hepatic stimulator substance (h-HSS) and liver cytosol (h-FLC) on the survival rate,the intensity of liver damage,and liver regeneration in rats with liver necrosis induced with D-galactosamine were observed.The physico-chemical properties of h-HSS were preliminarily determined.It was found that both h-HSS and H-FLC could markedly increase the.survival rate of the rats with toxic liver necrosis,significantly decrease the serum level of ornithine carbamoyl transferase and mitochondria aspartic trans-aminase,improve the hepaplastin activity,and elevate the incorporation rate of 3H-thymidine with liver DNA.h-HSS was stable after heated to 95℃ for 30 minutes,and sensitive to trypsin treatment.It demonstrated one major band at 15 000 daltons and 3 minor ones at 24 000,34 000,and 40 000 daltons respectively on sodium dodecyl sulfate-polyacrylamide gel electrophoresis.The relative content of the major band was 47.57%.

EFFECTS OF TESTOSTERONE OR ESTRADIOL AND COLCHICINE ON TOXIC HEPATIC NECROSIS IN DIFFERENT SEXUAL MICE / 第二军医大学学报

Toxic hepatic necrosis (THN) was induced in BALB/C mice by CCl4 infection (i.p.) and then colchicine (orally), testosterone propionate or estradiol valerate (s.c.) were administered. The experimental results reveal that the effect of colchicine on THN was better in male mice than that in femalemice.Testosterone propionate had no effect on the survival rate of male and female mice with THN, but enhanced the therapeutic effect of colchicine on THN in female mice. All male mice with estradiol died and half of them with estradiol and colchicine survived(P

EFFECTS OF DIFFERENT VITAMIN E CONTAINING DIETS ON LIVER NECROSIS INDUCED BY D-GALACTOSAMINE IN RATS / 营养学报

The effects of different vitamin E(VE) containing diets on lipid peroxi-dation and the degree of liver damage in rats were observed in the model of liver necrosis induced by galactosamine(D-GAL).The results were as follows: After 8 weeks, the basic levels of VE in plasma and liver tissue of the group fed VE-supplemented diet were significantly higher than that of the group fed normal VE diet, in the company with a significant lower basic plasma MDA level. At each time interval after D-GAL injection, the plasma and liver VE levels were found higher in the VE-supplemented group, and the content of GSH as well as the activities of SOD, GSH-Px, HPT were maintained satisfactorily while the MDA content and OCT, m-AST activities were very significantly lowered and the pathological changes of the liver were not so severe as compared with that in the normal VE diet group. It was noteworthy that the changes of almost all the parameters metioned above in the group fed VE-deficient diet went to an opposite direction to the VE-supplemented group. The results indicated that the VE-supplemented diet has a good liver protective effect, which may be established on the increased VE content and suppressed lipid peroxidation in the liver. VE-deficient diet could sharply reduce the VE content in the body and significantly intensify the susceptibility of liver to the harmful agents.