ABSTRACT
Objective To study percutaneous absorption kinetics and synergistic penetration enhancement of tetrahydropalmatine,aconitine and ginsenoside Rg1.Methods The in vitro intact and stripped skin permeation experiments were carried out by using Permcell KH-5P horizontal proliferation system.The percutaneous kinetic parameters of tetrahydropalmatine,aconitine and ginsenoside Rg1 were determined by HPLC,and the transdermal behaviors of the three active ingredients and interactions of combination were investigated.Result The cumulative amounts in 24 h of intact skin of tetrahydropalmatine,aconitine and ginsenoside Rg1 were 123.95,50.32,96.25(?10-10 cm2/s).The cumulative amounts in 24 h of stripped skin of them were 539.00,93.60,354.70(?10-10 cm2/s).The LogP were 1.66,0.66 and 0.57 respectively.Each component had cumulated amount permeated of improvement by 64%～293% in coexistence system of three active ingredients.Conclusion The combination of three active ingredients results in a synergistic enhancement effect on percutaneous absorption of every active ingredient,and it is shown that the maximal enhancement effect appears between aconitine and ginsenoside Rg1.