Web Arching Project of Japanese National Diet Library and its enlightenments / 中华医学图书情报杂志

After the background, features, characteristic projects and achievements of Web Arching Project (WARP) of Japanese National Diet Library were described,the experiences and significance of WARP were sum-marized for the collection and preservation of network resources in our country.

Study on the Long-term Preservation Storage Strategy of Medical Digital Resources / 医学信息学杂志

The necessity of long-term preservation of medical digital resources is analyzed from 4 aspects in the paper,namely,policies and regulations,strategic reserves,scientific findings verification and resource utilization.Based on the OAIS reference model,the storage function entities and the relationships are clarified.Through study of storage mode,architecture,technology and guarantee mechanism,scientific storage strategy is explored to realize the long-term reliable storage for medical digital resources.

Research on the Long-term Preservation Architecture of Medical Digital Resources / 医学信息学杂志

Through combing the objects of the medical digital resources of long-term preservation,analyzing the characteristics of medical digital resources,and discussing challenges coming from medical big data,the paper researches the long-term preservation architecture of medical digital resources primarily from life cycle,standard specification,storage architecture,monitoring management and preservation mode to provide effective guidance for comprehensive long-term preservation construction of medical digital resources.

Long-term preservation of digital resources in domestic libraries:A review / 中华医学图书情报杂志

After the necessity and importance for the long-term preservation of digital resources were described,the hot spots in studies on the long-term preservation of digital resources were summarized, including preservation pro-jects, preservation models and preservation strategies, followed by an analysis of their characteristics and limitations.

Viability and molecular authentication of Coccidioides spp. isolates from the Instituto de Medicina Tropical de São Paulo culture collection, Brazil / Viabilidade e autenticação molecular de isolados de Coccidioides spp. da coleção de culturas do Instituto de Medicina Tropical de São Paulo, Brasil

Coccidioidomycosis is an emerging fungal disease in Brazil; adequate maintenance and authentication of Coccidioides isolates are essential for research into genetic diversity of the environmental organisms, as well as for understanding the human disease. Seventeen Coccidioides isolates maintained under mineral oil since 1975 in the Instituto de Medicina Tropical de São Paulo (IMTSP) culture collection, Brazil, were evaluated with respect to their viability, morphological characteristics and genetic features in order to authenticate these fungal cultures. Only five isolates were viable after almost 30 years, showing typical morphological characteristics, and sequencing analysis using Coi-F and Coi-R primers revealed 99% identity with Coccidioides genera. These five isolates were then preserved in liquid nitrogen and sterile water, and remained viable after two years of storage under these conditions, maintaining the same features.

Coccidioidomicose é uma doença emergente no Brasil; a manutenção adequada e autenticação de isolados de Coccidioides spp são essenciais para a pesquisa em diversidade genética de micro-organismos, bem como para a compreensão da doença em humanos. Dezessete isolados de Coccidioides preservados em óleo mineral desde 1975 na coleção de culturas do Instituto de Medicina Tropical de São Paulo (IMTSP) foram avaliados com relação à viabilidade, características morfológicas e genéticas, com o objetivo de autenticação das culturas fúngicas. Dos 17 isolados, apenas cinco foram viáveis após quase 30 anos mantidos em óleo mineral, apresentando características morfológicas e moleculares típicas do gênero, o sequenciamento utilizando os oligonucleotídeos Coi-F e Coi-R revelou identidade de 99% com isolados de Coccidioides. Estes cinco isolados foram preservados em nitrogênio líquido e água destilada esterilizada, e permaneceram viáveis após dois anos de armazenamento sob estas condições, mantendo as mesmas características.

The feasibility of platelet long cryopreserved at-80℃ / 中国输血杂志

0.05).Conclusion Platelets maintain a good and stable quality at-80 ℃ and the-80 ℃ refrigerator may cryopreserve the platelets for a long time.