ABSTRACT
Objective:To research the TLC identification method for Longdan Xiegan pills( honey pills) . Methods:TLC was used in the identification. The samples were extracted by 70% methanol with a heating reflux method, and then extracted by the agents with dif-ferent polarity, including petroleum ether, ethyl acetate and butanol. The petroleum ether part was detected by fluorescence at 365nm for Angelica sinensis, and 1% vanillin-sulfuric acid color reaction was used to detect Alisma orientale. The ethyl acetate part was determined by fluorescence at 365nm for Scutellaria baicalensis, and the butanol part was detected with chloroform-methanol-water (30∶12∶3) as the developing solvent for Bupleurum and Glycyrrhiza, and with acetone-ethyl acetate-water (6∶6∶1) as the developing solvent for gentiopi-croside, geniposide and liquiritin. Results:The developed TLC spots were clear with good separation, high specificity and promising re-producibility. Conclusion:The method can be exactly used in the qualitative identification and quality control of Longdan Xiegan pills ( honey pills) .
ABSTRACT
OBJECTIVE:To investigate the equivalence of preparations of traditional Chinese medicines with decoction and pill as the representative dosage forms so as to lay a foundation for the evaluation of equivalence.METHODS:Taking the contents of gentiopicroside and geniposide and water soluble extractive as indexes to investigate the equivalence between decoction for Longdan xiegan tang and Longdan xiegan pills.RESULTS:Evaluated by the indexes of contents of gentiopicroside and geniposide,the current daily oral dosage of the decoction was 6.39 times superior to the pill;taking water soluble extractive as index,the decoction was 4.02 times superior to the pill.CONCLUSION:To be equivalent to the decoction,the current daily oral dosage of the pill should be increased appropriately.The degree of increase should be based on the final results of pharmacological and clinical studies.