ABSTRACT
Objective To study the immunomodulatory effects of Longdan Xiegan Tang on related inflammatory cytokines in rots with experimental autoimmune uveitis (EAU).Methods Lewis rats were randomly divided into control group (6 rats),EAU group (18 rats) and LXT group (18 rats).Rats in EAU and LXT groups were immunized with interphotoreceptor retinoid-binding protein (IRBP) emulsion.The expressions of IFN-γ,IL-17,IL-10 and TNF-α were investigated by quantitative real-time PCR and ELISA,respectively.Results In blood of LXT group,the mRNA level of IFN-γ on day 8 was higher than that in control group (P =0.000),but obviously lower than that in EAU group (P =0.000);The mRNA level of IL-17 was peaked on day 12,but was lower than EAU group (P=0.000);The mRNA level of TNF-α on day 12 was higher than that in control group (P =0.000),but obviously lower than that in EAU group (P =0.000);The mRNA level of IL-10 on day 16 was higher than that in EAU group (P =0.042).In lymph node and spleen of LXT group,the mRNA levels of IFN-γ,IL-17 and TNF-α on day 12 were lower than those in EAU group (all P=0.000);The mRNA level of IL-10 was peaked on day 12,but was lower than EAU group (P =0.000).In serum of LXT group,the mRNA levels of IFN--γ,IL-17 and TNF-α on day 12 and day 16 were lower than those in EAU group;The level of IL-10 in EAU and LXT group on day 12 were higher than that in control group,peaked on day 16,but the LXT group was the highest.Conclusion Longdan Xiegan Tang can reduce the expressions of proinflammatory cytokines including IFN-γ,IL-17 and TNF-α.Meanwhile,it can also promote the production of IL-10 and further accelerate the recovery of EAU,indicating that Longdan Xiegan Tang can play a significant role in treating uveitis.
ABSTRACT
OBJECTIVE:To observe therapeutic efficacy and safety of modified Longdan xiegan tang in the treatment of psoria-sis vulgaris(blood heat type). METHODS:A total of 80 patients with psoriasis vulgaris(blood heat type)were randomly divided into control group(38 cases)and observation group(42 cases),other 30 healthy volunteers were included in healthy control group. Control group was given Xiaoyin tablet 1.6 g orally,3 times a day for 4 weeks. Observation group was given modified Longdan xiegan tang,400 mL after detected with water,1 h after breakfast and supper,7 d as a treatment course,for 4 courses. Clinical ef-ficacies and PASI score were observed before and after treatment. The levels of inflammatory factors(IL-1,IL-6,IL-8,TNF-α), the expression of NF-κB protein and NF-κB gene were observed in observation group before and after treatment,and then were compared with healthy control group. The relationship of inflammatory factor levels with NF-κB protein expression in observation group before treatment,the relationship of PASI score with inflammatory factor and NF-κB expression were observed in observa-tion group before and after treatment,and the occurrence of ADR was recorded. RESULTS:Total response rate of observation group(85.7%)was significantly higher than that of control group(52.6%),with statistical significance(P<0.05). Before treat-ment,there was no statistical significance in PASI score between 2 groups (P>0.05). After treatment,PASI score of 2 groups were significantly lower than before treatment,and the observation group was significantly lower than the control group,with statis-tical significance (P<0.05). After treatment,The levels of IL-1,IL-6,IL-8 and TNF-α in observation group were significantly lower than before treatment,but there was no statistical significance with healthy control group(P>0.05);the expression of NF-κB protein and NF-κB gene in observation group were significantly higher than healthy control group,with statistical significance(P<0.01 or P<0.05). The levels of IL-1, IL-6, IL-8 and TNF-α were positively associated with the expression of NF-κB protein in observation group before treatment. The levels of IL-1,IL-6,IL-8 and TNF-α and the expression of NF-κB pro-tein were both positively associated with PASI score in observa-tion group before and after treatment. CONCLUSIONS:Modified Longdan xiegan tang show significant therapeutic efficacy for psoriasis vulgaris(blood heat type),can reduce PASI score,the levels of NF-κB protein expression and inflammatory factors.
ABSTRACT
OBJECTIVE:To investigate the equivalence of preparations of traditional Chinese medicines with decoction and pill as the representative dosage forms so as to lay a foundation for the evaluation of equivalence.METHODS:Taking the contents of gentiopicroside and geniposide and water soluble extractive as indexes to investigate the equivalence between decoction for Longdan xiegan tang and Longdan xiegan pills.RESULTS:Evaluated by the indexes of contents of gentiopicroside and geniposide,the current daily oral dosage of the decoction was 6.39 times superior to the pill;taking water soluble extractive as index,the decoction was 4.02 times superior to the pill.CONCLUSION:To be equivalent to the decoction,the current daily oral dosage of the pill should be increased appropriately.The degree of increase should be based on the final results of pharmacological and clinical studies.