ABSTRACT
Objective To study the effect of assisted thoracic small incision on serum CK19 mRNA and Lunx mRNA in patients with lung cancer.Methods 90 patients with lung cancer were randomly divided into the control group and the observation group.45 patients in the control group were operated through thoracotomy,while 45patients in the observation group were operated through assisted thoracic small incision.Serum CK19 mRNA and Lunx mRNA were detected before and after operation through RT-PCR.Results Serum CK19 mRNA and Lunx mRNA were decreased after operation (t =19.682,12.784,7.824,7.085,all P ＜ 0.01).And the mRNA levels in the observation group were lower than those in the control group (t =1.900,1.438,all P ＜ 0.05).Conclusion Assisted thoracic small incision can decrease serum CK19 mRNA and Lunx mRNA in patients with lung cancer,which can improve micrometastasis.
ABSTRACT
BACKGROUND: To enhance the cancer cell detection rate in blood, we tried to detect cancer cells by blood filtration, RNA extraction and reverse transcription (RT)-PCR in the filtered mononuclear cells (MNCs). METHODS: From the specimens of whole blood and filtered MNCs, RNA was extracted by the guanidinium isothiocyanate buffer method. Filtration efficiency was evaluated by measurement of leukocyte count, red cell count, and hemoglobin level. To compare the RNA extraction efficiency between whole blood and filtered MNCs, the followings were examined: (1) RNA electrophoresis, (2) hTERT, survivin, plakophilin, LunX, and MAGE A1-6 RT-PCR, and (3) the detection limit of added SNU484 cells in the blood by MAGE A1-6 RT-PCR. Finally blood specimens of 13 lung cancer patients were used to detect cancer cells by LunX and MAGE A1-6 RT-PCR with filtered MNCs. RESULTS: The filtration method revealed 0%, 92.8% and 95.1% filtration rates for leukocyte, red cell, and hemoglobin, respectively. Contamination of concentrated genomic DNA was observed in the electrophoresis of RNA extracted from whole blood. Positive rates of hTERT, survivin and plakophilin were higher in the filtered MNCs. The filtration method detected 1 SNU484 cell/mL, and the blood samples of 4 (30.8%) lung cancer patients showed positive results for RT-PCR. CONCLUSIONS: For the detection of cancer cells in the blood, the filtration method was very efficient, and LunX and MAGE A1-6 genes would be useful for the detection of blood cancer cells in patients with lung cancer.