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AiM: To observe the recruitment effect of lung cancer circulating tumor cells (CTCs) on neutrophils, and find out the effective components of jinfukang in inhibiting the recruitment of neutrophils by CTCs. METHODS: The ability of human lung adenocarcinoma circulating tumor cells CTC-TjH-01 cells to recruit neutrophils from whole blood leukocytes, and the effect of jinfukang and its six active ingredients on the recruitment of neutrophils by CTCs was detected by flow cytometry. CCK-8 assay was used to observe cell viability to determine the concentration of action; transwell chemotaxis assay was used to detect the effect of six active ingredients on the chemotaxis of CTC-TjH-01 cells to neutrophils. RESULTS: CTCTjH-01 cells could increase the recruitment of neutrophils compared to blank control (p < 0.01); after the effect of jinfukang, the neutrophils recruited by CTC-TjH-01 cells decreased (p < 0.01). Trigonelline and Ophiopogonin W reduced neutrophil recruitment to CTC-TjH-01 cells at concentrations that had no effect on cell viability (p < 0.01), trigonelline had the best effect; the chemotaxis of CTC-TjH-01 cells to neutrophils was weakened by trigonelline, astragaloside IVz and Ophiopogon pol-ysaccharide (p < 0.05), and trigonelline had the best effect. CONCLUSiON: jinfukang can inhibit the recruitment of neutrophils by circulating tumor cells, and trigonelline, an effective monomer with "Fuzheng" effect in jinfukang, can significantly inhibit the recruitment of neutrophils by circulating tumor cells in lung cancer, which proves that trigonelline may have the potential to inhibit lung cancer metastasis through targeting neutrophils.
ABSTRACT
Postoperative asymptomatic patients with early cancer (lung cancer) have dormant disseminated tumor cells (DTCs) in their metastatic target organs, and the proliferation of these DTCs is the key link leading to clinical metastasis. The development of therapeutic agents to maintain DTCs dormant or eradicate dormant DTCs will prevent tumor metastasis and break through the bottleneck of improving the overall efficacy of treating malignant tumors. This paper reviews the methods of establishing in vitro and in vivo research models of DTCs with dormant characteristics to promote the understanding of dormant DTCs and improve the research and development efficiency of anti-tumor metastasis drugs.
ABSTRACT
Lung cancer is one kind of malignant tumors with the highest morbidity and mortality, and lung cancer metastasis is the leading cause of death in patients. Lung cancer metastasis is a complex process. A large number of studies have confirmed that lung tumors can secrete soluble molecules, mobilize and recruit related bone marrow cells or immune cells, regulate the matrix remodeling in distant organs which leads to the formation of pre-metastatic niche, therefore, promote tumor cells metastasis and implantation in distant organs. Detection of key target factor molecules and cellular components involving in pre-metastatic niche is of great clinical significance for diagnosis and prognostic evaluation. This article will summarize the progression of lung cancer metastasis modulated by the pre-metastatic niches and its clinical application status.
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Objective: To observe the intervention of total flavonoids from Camptosorus sibiricus (TFCS) on lung cancer in mice and to study its possible antitumor mechanism. Methods: The lung cancer model induced by urethane was used to investigate the preventive action of TFCS on lung cancer, the passive pulmonary metastatic model of Lewis lung cancer was used to evaluate the effect of TFCS on tumor metastasis, the scavenger effect of TFCS on system microenvironment was observed in the recurrent tumor model of Lewis lung cancer, the effect of TFCS on lysyl oxidase (LOX) was investigated by Western blotting and immunohistochemical staining, and the vascular normalization function of TFCS was examined by capillary permeability in carcinogenetic lung tissues. Results: TFCS (30 and 100 mg/kg) could decrease the lung carcinogenesis induced by urethane and reduce the passive pulmonary metastasis and recurrence after tumor removal in Lewis lung cancer-bearing mice. TFCS (30 and 100 mg/kg) could also downregulate the expression of LOX in lung cancer tissue, prevent serum lipid peroxide formation in mice with tumor removal, and promote vascular normalization of lung cancer tissue. Conclusion: TFCS as an LOX inhibitor has a definite preventive action on lung cancer.
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The process of lung cancer invasion and metastasis consists of a complex series of multigene and multistep, CD44V6 is a broadly distributed transmembrane glycoprotein, which mainly participates in the specific adhesion process and plays an important role in cell-cell and cell-matrix interactions.The researches showed that the high expression of CD44V6 was helpful for metastasis ability of tumor cell. MMps is a groups of metal hydronium depend-proteinase, it exhibits proteolytic activity against components of the extracellular matrix (ECM). The recent study indicated that MMps has been played important roles not only in degradation of ECM proteins, but also in the regulation of tumorigenesis. Combined with two marker might be an important significant to judge prognosis ,metastasis and development.
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Objective To explore the PGI 2 and TXA 2 for lung cancer patients and ones with lung cancer metastasis,in onder to provide nomal index for diagnosis and differential diagnosis in lung cancer metastasis.Methods To test plasma PGI 2 and TXA 2 by means of RIA in all cases.One group of patients who received non operative treatment:Six cases of patients with distant metastasis;Eight cases with hilus of long metastasis;Seven cases without metastasis.The other group of patients who received radical operation;Six cases of patients with distant metastasis;Seven cases with hilus of lung metastasis;Six cases without metastasis.Result The PGI 2/TXA 2 ratio of the patient whether distant metastasis or hilus of lung metastasis was obviously lower than that of the patients without metastasis( P