ABSTRACT
Objective To investigate the methylation status of ZIC1 gene in peripheral blood and lung cancer tissues of NSCLC patients and its prognostic significance. Methods We took the peripheral blood, cancer tissues and adjacent tissues of 95 NSCLC patients. The peripheral blood of 95 healthy people was taken as control group. MSP was used to compare the detection rate of ZIC1 methylation between peripheral blood and cancer tissues. And we analyzed the correlation of ZIC1 methylation in peripheral blood and cancer tissues with the clinicopathological factors of NSCLC patients. Results The methylation detection rates of ZIC1 in peripheral blood and lung cancer tissues in NSCLC patients were significantly higher than those in peripheral blood of healthy people and adjacent tissues of NSCLC patients (P < 0.05), and the sensitivity and specificity of ZIC1 methylation in the diagnosis of tumor tissues were higher. The positive rate of ZIC1 gene methylation in peripheral blood and tumor tissues of NSCLC patients was significantly correlated with tumor diameter, metastasis, stage and pleural effusion (P < 0.05). Conclusion The methylation of ZIC1 gene is related to the occurrence, development, metastasis and stage of NSCLC. It may be used as a diagnostic and prognostic indicator of NSCLC.
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Objective To investigate the relationship between metalloproteinase-9-9 (Matrix),inhibitor-1-1 (MMP-9),metalloproteinase TIMP-1 factor (Matrix) and endothelial growth (VEGF),and to investigate the correlation between the expression of (matrix) and vascular endothelial growth factor (endothelial) and vascular endothelial growth factor (vascular).Methods Sixty-eight cases of non small cell lung cancer patients treated in fengning manchu autonomous county hospital from January to January 2014 were analyzed retrospectively,and the expression level of TIMP-1,VEGF and MMP-9 were analyzed by immunohistochemical method.Results The positive rates of MMP-9 (75％),TIMP-1 (19.12％) and VEGF (13.24％) were significantly higher than those in normal tissues (P＜0.05),MMP-9 (60.87％),VEGF (54.55％),TIMP-1 (65.22％) and VEGF (76.09％,59.09％,63.64％).The positive rates ofTIMP-1,VEGF and MMP-9 were higher than those without lymph node metastasis (P ＜0.05) Conclusion MMP-9,TIMP-1 and VEGF expression levels can predict the invasion and metastasis of non small cell lung cancer,and the three kinds of substances can promote the development and progression of lung cancer in different degrees.
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Objective Microheterogeneity is an important problem in molecularly biological and proteomic research on malignancies.The goal of this study was to investigate the method to purify targeted cells in lung cancer and its paired normal tissues for proteomic study on lung cancer applying laser capture microdissection(LCM).Methods LCM technique was emploged to obtain the cells of lung cancer tissues and their paired normal tissues from frozen sections.Results LCM can be applied to quickly and precisely obtain pure targeted cells subgroup or single cell without interstitium under the microscope.Conclusion LCM can tackle the problem of tissue heterogeneity in molecular analysis successfully.These results indicate that LCM has a potential as a tool in lung cancer proteomic research.
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Objective To investigate the diagnostic value of tissue polypeptide specific antigen (TPS),CYFRA21-1 and soluble tumor necrosis factor receptor (STNFR) in patients with lung cancer.Methods The serum levels of TPS,CYFRA21-1 and STNFR were determined by enzyme-linked immunosorbent assay (ELISA) in 72 patients with lung cancer,54 patients with benign diseases and 32 healthy adults.Results The levels of the 3 tumor markers in lung cancer group were significantly higher than those of the benign disease group and healthy control group,and the serum levels of this 3 tumor markers corresponding increased with the high TNM stage.The detectable rates of TPS (80.5%) and STNFR (81.9%) in serum of lung cancer were higher than CYFRA21-1(65.3%),and in TNM I group,the detectable rates of TPS (57.1%) and STNFR (57.2%) were higher than CYFRA21-1 (25.6%) too.Conclusion The TPS,CYFRA21-1 and STNFR can be used as a very useful and sensitive tumor marker in the diagnosis of lung cancer.The TPS and STNFR are better than CYFRA21-1 in clinical use.