ABSTRACT
Objective: To study the chemical constituents in the whole herb of Lycopodium japonicum. Methods: The chemical constituents were separated and purified by silica gel, Sephadex LH-20 column chromatography, and preparative HPLC. Their structures were identified by various spectroscopic analyses. Results: Thirteen compounds were obtained from the whole herb of L. japonicum by the chromatographic methods on silica gel, ODS, and Sephadex LH-20 column, and preparative HPLC. According to physicochemical properties and spectral data, these compounds were identified as betulin (1), di-(2-ethylhexyl) phthalate (2), α-onocerin (3), 16-oxo-3α-hydroxyserrat-14-en-21β-ol (4), 3-epilycoclavanol (5), (24S)-24-methyl cholesterol (6), lycopodiin A (7), tomentosanan B (8), α-obscurine (9), lycoclavanol (10), serratenediol (11), 1, 2-diarylpropane-3-ol (12), and 13, 13-ethylenedioxy-15, 16-dinwlabd-7-en-6β-ol (13). Conclusion: Compounds 1, 2, 6, 8, and 11-13 are isolated from this plant for the first time. Compounds 2, 6, 8, and 12 are isolated from the plants of this genus for the first time.
ABSTRACT
Objective: To investigate the chemical constituents in the whole plant of Lycopodium japonicum, native to Suizhou city, Hubei province for the first time. Methods: The compounds were isolated and purified by a combination of silica gel, ODS, and Sephadex LH-20 column chromatography and prep-HPLC. Their chemical structures were established by their physicochemical properties and spectral data. Results: Eight pentacyclic triterpenoids have been isolated and characterized from the methanol extract of the whole plant, they are 21-episerratenediol (1), serratenediol (2), α-onocerin (3), serrat-14-en-3β, 21β, 24-triol (4), 3β, 21α- dihydroxyserrat-14-en-16-one (5), lycoclavanol (6), lyclaninol (7), and 3α, 21β, 24-trihydroxyserrat-14-en-16-one (8). Conclusion: Compounds 1-8 are all serretene triterpenoids, the characteristic chemical constituents in the plants of genus Lycopodium L. Compound 5 is isolated from this plant for the first time.