ABSTRACT
Electrical stimulation (ES) are known to have beneficial effects in wound healing. However, the effect of electrical stimulation in wound theraphy on the oxidant and antioxidant levels of various tissues in the body remains unclear. Here, we investigated the effects of electrical stimulation on the oxidative stress capacity of tissues in a rat model with a surgical incision wound on the lateral line of the femoral region. Rats divided in two groups: control and ES group. A longitudinal skin incision was made only from the right lateral line of all rats. ES was applied 200 ?s, 20 Hz, 2mA for 20 min during 15 days. Some oxidative stress parameters (malondialdehyde (MDA), reduced glutathione (rGSH), glucose-6-phosphate dehydrogenase (G6PD), glutathione peroxidase (GPx) and catalase (CAT)) in tissues (brain, heart, kidney, liver, testis, gastrocnemius and quadriceps femoris muscle) were obtained spectrophotometrically. The ES reduced oxidative stress by decreasing MDA and simultaneously increasing different antioxidants in different tissues. The results suggest that incision wound could induce oxidative stress in tissues and electrical stimulation post-incision wound may have ameliorative effect.
ABSTRACT
The maximal isometric strength of the knee extension (KES) and cross-sectionl area of m. quadriceps femoris (MQF) were measured on 89 male Japanese elite athletes and 14 untrained men to evaluate the morphological and functional characteristics of athletes in the knee extensor muscles. Athletes were specialized in seven different sport events; 16 sumo wrestlers, 22 oarsmen, 8 volleyball players, 16 speed skaters, 12 association football plyers, 5 sprinters, 10 middle and long distance runners. KES was measured by using a specially designed straingages dynamometer at knee angle of 110 degrees (180 degrees= fully extended) . Cross-sectional area of MQF at the mid-thigh was determined by ultrasonic apparatus. The following results were obtained.<BR>1. Sumo wrestters showed highest mean values (110.18 cm<SUP>2</SUP>) in MQF area among athlete groups. Lower mean values in MQF area were found in sprinters (86.34 cm<SUP>2</SUP>) and distance runners (73.86 cm<SUP>2</SUP>) whose values were not significantly different from that of untrained (75.32 cm<SUP>2</SUP>) .<BR>2. In the cross-sectional area of each muscle bundle of MQF, sumo wrestlers, volleyball players and speed skaters showed higher mean values, and sprinters and distance runners had lower mean values as well as untrained men.<BR>3. Higher mean values in percentage of MQF area to the total muscle area of thigh were observed in volleyball players (58.66%) and oarsmen (57.53%), lower mean value in association football players (53.81%), respectively. However, there were no significant differences in percentage of MQF area between untrained and each athlete group.<BR>4. In the percentage of each muscle bundle area to MQF area, m. rectus femoris of association football players and m. vastus lateralis of speed skaters were significantly higher mean values compared with the untrained and other athlete groups.<BR>5. KES were significantly related to the MQF area at 0.1% level (r=0.657) . There were significant relationships KES and each muscle bundle area in MQF area at 0.1% level. In the relationship between KES and each muscle bundle area, m. vastus lateralis showed the highest correlation coefficient (r=0.603) .<BR>6. Association football players showed highest mean value (8.97 N/cm<SUP>2</SUP>) in KES per unit of MQF area among athlete groups. Its value was significantly higher than that of untrained men (8.06 N/cm<SUP>2</SUP>), distance runners (7.31 N/cm<SUP>2</SUP>), volleyball players (7.10N/cm<SUP>2</SUP>), and sumo wrestlers (7.50N/cm<SUP>2</SUP>) . There were no significant differences in KES per unit of MQF area intra athlete groups except for association football players.