ABSTRACT
Breast cancer is a disease caused by abnormal cell proliferation in the breast. God’s crown fruit (Phaleria macrocarpa)and its seed have potential as an antiproliferation of cancer cells. It contains active compounds such as flavonoids,alkaloids, polyphenols, and tannins. The sample of God’s crown fruit was obtained by extraction and fractionationusing the maceration method. Cytotoxicity of extracts and fractions was determined using Brine Shrimp Lethality Testmethod. Antiproliferation activity test of God’s crown fruit against MCM-B2 was performed using the hemacytometermethod. The God’s crown fruit sample consists of crude ethanol extract, n-hexane fraction, ethyl acetate fraction, andwater fraction. Lethal concentration 50 (LC50) values in crude ethanol extract, n-hexane fraction, ethyl acetate fraction,and water fraction were 13.72, 147.55, 405.81, and 149.55 ppm, respectively. The concentration of the test sample wasdirectly used for the antiproliferation activity test on MCM-B2 cells. God’s crown fruit can act as antiproliferation ofMCM-B2. The smallest concentration of those samples has inhibited MCM-B2 cell proliferation which is 3.5 ppmcrude ethanol extract lower than 100 ppm doxorubicin. The maximum percentage of the antiproliferation activity ofcrude ethanol extract (56 ppm) was able to inhibit MCM-B2 cell proliferation by 58.28% while doxorubicin (100 ppm)by 31.2%. This is due to the fact that crude ethanol extract has a lot of complex polar phytochemical content. The crudeethanol extract compounds inhibit MCM-B2 cell proliferation synergistically
ABSTRACT
The antiproliferative of the brown algae Padina australis extracts against cell MCM-B2 (canine benign mammarygland mixed tumor) and cell K562 (human chronic myelogenous leukemia) in vitro was examined. The tested sampleswere water extract (n-hexane fraction, ethyl acetate fraction, and ethanol fraction) and ethanol extract (n-hexanefraction, ethyl acetate fraction, and ethanol fraction). Cytotoxicity was screened using brine shrimp lethality test.The antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl. The antiproliferative activity test wasconducted using trypan blue dye method and cells were counted using hemocytometer. The results showed thatethyl acetate fraction from water extract exhibited significant cytotoxicity with lethal concentration 50 value of200.53 ppm. The ethyl acetate fraction from water extract was then considered for further examination. Results ofantioxidant activity test showed that concentration for inhibitory activity of 50% of the ethyl acetate fraction reached113.37 ppm. This fraction at concentration of 400 ppm could inhibit the growth of MCM-B2 and K562 cancer celllines in vitro reaching 56.90% and 61.54%, respectively. Therefore, the present study suggested that ethyl acetatefraction of P. australis extract demonstrated a potential natural anticancer activity.