Minichromosome maintenance 7 protein is a reliable biological marker for human cervical progressive disease / 부인종양

OBJECTIVE: This study focused on comparing the expression levels of p16, Ki-67, and minichromosome maintenance 7 (MCM7) protein in normal and affected cervical epithelium to ascertain the biological significance of these markers in detecting progressive cervical disease. METHODS: A quantitative and based on-scanning-microscopy analysis of the three markers expression was performed in normal and cervical intraepithelial neoplasia (CIN) I, II, and III tissues. p16 area as well as p16, Ki-67, and MCM7 positive cells or nuclei were evaluated according to their distribution and extent through the cervical epithelium. RESULTS: A clear p16 over-expression was observed in all the dysplastic epithelium tissue samples. The quantitative analysis of p16 area as well as the number of p16 positive cells was able to better discriminate the CIN lesions grades than the usual semi-quantitative analysis. The average Ki-67 labeling indexes for the normal epithelium, CIN I, CIN II, and CIN III groups were 19.8%, 27.3%, 32.8%, and 37.1%, respectively, whereas the mean MCM7 labeling indexes for the correspondent grades were 27.0%, 30.4%, 50.5%, and 67.2%. The Ki-67 and MCM7 labeling indexes were closely correlated with the CIN histological grade, with higher labeling indexe values obtained from the more severe lesions (p<0.05), being the MCM7 labeling indexes the highest values in all the CIN categories (p<0.05). CONCLUSION: We observed a good correlation among the p16, Ki-67, and MCM7 data. In addition, MCM7 demonstrated to be a more efficient and sensitive marker to assess disease progression in the uterine cervix.

The Expression and Clinical Significance of the Minichromosome Maintenance (MCM) 7 Proliferation Markers in Urothelial Carcinomas of the Bladder / 대한비뇨기과학회지

PURPOSE: The antibodies for minichromosome maintenance(MCM) proteins have been reported as potential proliferative markers and prognostic indicators in various human malignancies. The present study examined the expression pattern of MCM proteins in bladder carcinomas, and we also evaluated their prognostic significance as well as their potential applicability as proliferation markers. MATERIALS AND METHODS: Immunohistochemistry for MCM7 and Ki-67 was performed on paraffin sections from 47 cases of bladder carcinoma. The MCM7 and Ki-67 expressions were quantified and then analysis was carried out for determining the association between the expressions of MCM7 and Ki-67 and the clinicopathological parameters. RESULTS: A significant correlation existed between the expression rate of MCM7 and the histological grade(p<0.0001). The Ki-67 expression rate was significantly related to the tumor grade(p=0.002) and the pathological stage(p=0.011). On multivariate analysis, MCM7 was not found to be an independent prognostic factor for predicting the recurrence of bladder carcinoma. CONCLUSIONS: The results suggest that MCM is a reliable proliferation marker, but not an independent predictive factor for recurrence of bladder carcinoma.