ABSTRACT
Objectives:The purpose of the present study was to compare hydrolysis of structured lipid and physical mixed MCT/LCT with LCT emulsion. Methods:The emulsions used in this study were 20% Intralipid,20%Lipofundin and 20% Structoliped.Glycerol tri [1 14 C]palmitate and Glycerol trioctanoate were dissolved in lipid emulsions to give a specific radioactivity of 0.075 ?Ci/mg LCT and MCT oils.With buffer system at optimal pH,plasma albumin,lipoprotein lipase and hepatic lipases were added.Incubation was performed in a shaking water bath at 37℃ for 60 minutes.Rate of hydrolysis were initially expressed as the amount of FFA release measured by radioactivity count.Percent hydrolysis was calculated as 14 C labled LCFAs and 3H labled MCFAs released relative to the initial amount of 14 C labled LCTs and 3H labled MCTs in the incubation mixture respectively. Results:The physical mixed MCT/LCT and structured lipid emulsion were hydrolyzed better than pure LCT particles,and more free fatty acids were released( P 0.05). Conclusions:The rate of physical mixed MCT/LCT emulsion and structured lipid emulsion hydrolysis were better pure than LCT emulsion,the release of total FFAs during emulsion hydrolysis is largely dependent on the amount of MCTs.
ABSTRACT
Objectives: To compare the effect of different fat emulsions on the blood lipid and liver function in neonates with hepatic dysfunction. Methods:40 babies with hyperbilirubinemia(≥51.3 mmol/L) were randomly divided into two groups ,receiving the fat emulsion of 20% Intralipid (LCT group) or 20% Lipofundin (MCT/LCT group) at the dose of 2.0 g/(kg?d) for 7 days. Blood lipid 〔total cholesterol(TC), triglyceride(TG)〕 and the serum biochemical indexes relative to liver function were measured before and after the period of 7 days. Results:The levels of TC did not change remarkably in two groups. There was significant difference in the levels of TG between the two groups after the study period (1.87?0.3 mmol/L in LCT group and 1.15?0.27 mmol/L in MCT/LCT group, t =7.833, P