ABSTRACT
Objective To identify G?quadruplex structures in the promoter region of MET. Methods CD spectroscopy,UV spectroscopy,non?denatured electrophoresis and PCR stop assay were applied to indicate the G?quadruplex structure and its function. Results The Pu23WT se?quence in the promoter of MET adopted an intramolecular parallel G?quadruplex structure under physiological conditions in vitro,which can stop the extension of Pmet. Conclusion G?quadruplex structure in the promoter might inhibit MET gene expression in vivo.
ABSTRACT
Objective: To investigate the expressions of estrogen receptor (ER) subtypes and c-met proto-oncogene in human endometrial carcinomas and to assess the clinical significance of ER and c-met in this carcinoma. Methods: Reverse transcription PCR (RT-PCR) was used to detect the expressions of ERα, ERβ and c-met proto-oncogene mRNA in 30 samples of endometrial carcinoma and 11 samples of normal endometrium. Results: The expression of ERα in endometrial carcinoma (0.70±0.40) was significantly reduced in comparison to that in normal endometrium (1.14±0.56, P<0.05). A similar finding was made for the expression of ERβ in carcinoma (0.24±0.18) versus normal tissues (0.48±0.20, P<0.05). In contrast, c-met mRNA expression was increased in endometrial carcinoma (1.45±0.72) compared to that in normal endometrium (0.42±0.31, P<0.01). A decrease tendency of the expression of ERα was also found from Stage I (0.82±0.41) to a more severe Stag II-III of endometrial carcinoma (0.42±0.17, P<0.05). The analysis of ERα and ERβ mRNA revealed a decrease tendency from shallow to deep invasion of the uterine muscles (P<0.05). We found that the expressions of ERα and ERβ were negatively correlated with c-met proto-oncogene with a coefficient correlation of -0.63 (P<0.01) and -0.32 (P<0.05), respectively. Conclusion: ERα and ERβ are both involved in mutagenic action of carcinogen. C-met proto-oncogene plays an important role in the carcinogenesis and development of endometrial carcinoma. C-met and ER expressions show a negative correlation in the development of endometrial carcinoma.
ABSTRACT
Objective To investigate the expressions of estrogen receptor (ER) subtypes and c-met proto-oncogene in human endometrial carcinomas and to assess the clinical significance of ER and c-met in this carcinoma. Methods Reverse transcription PCR (RT-PCR) was used to detect the expressions of ERα, ERβ and c-met proto-oncogene mRNA in 30 samples of endometrial carcinoma and 11 samples of normal endometrium. Results The expression of ERα in endometrial carcinoma (0.70±0.40) was significantly reduced in comparison to that in normal endometrium (1.14±0.56, P<0.05). A similar finding was made for the expression of ERβ in carcinoma (0.24±0.18) versus normal tissues (0.48±0.20, P<0.05). In contrast, c-met mRNA expression was increased in endometrial carcinoma (1.45±0.72) compared to that in normal endometrium (0.42±0.31, P<0.01). A decrease tendency of the expression of ERα was also found from Stage Ⅰ (0.82±0.41) to a more severe Stag Ⅱ-Ⅲ of endometrial carcinoma (0.42±0.17, P<0.05). The analysis of ERα and ERβ mRNA revealed a decrease tendency from shallow to deep invasion of the uterine muscles (P<0.05). We found that the expressions of ERα and ERβ were negatively correlated with c-met proto-oncogene with a coefficient correlation of -0.63 (P<0.01) and -0.32 (P<0.05), respectively. Conclusion ERα and ERβ are both involved in mutagenic action of carcinogen. C-met proto-oncogene plays an important role in the carcinogenesis and development of endometrial carcinoma. C-met and ER expressions show a negative correlation in the development of endometrial carcinoma.