Construction and performance analysis of a microbial electrochemical sensor for monitoring heavy metals in water environment / 生物工程学报

A microbial fuel cell (MFC)-based microbial electrochemical sensor was developed for real-time on-line monitoring of heavy metals in water environment. The microbial electrochemical sensor was constructed with staggered flow distribution method to optimize the parameters such as external resistance value and external circulation rate. The inhibition of concentration of simulated heavy metal wastewater on voltage under optimal parameters was analyzed. The results showed that the best performance of MFC electrochemical sensor was achieved when the external resistance value was 130 Ω and the external circulation rate was 1.0 mL/min. In this case, the microbial electrochemical sensors were responsive to 1-10 mg/L Cu2+, 0.25-1.25 mg/L Cd2+, 0.25-1.25 mg/L Cr6+ and 0.25-1.00 mg/L Hg2+ within 60 minutes. The maximum rejection rates of the output voltage were 92.95%, 73.11%, 82.76% and 75.80%, respectively, and the linear correlation coefficients were all greater than 0.95. In addition, the microbial electrochemical sensor showed a good biological reproducibility. The good performance for detecting heavy metals by the newly developed microbial electrochemical sensor may facilitate the real-time on-line monitoring of heavy metals in water environment.

Effects of Limonin on Immune Function and Apoptosis-related Factors Expression in MFC Gastric Cancer Bearing Model Mice / 中国药房

OBJECTIVE：To study the effects of limonin on immune function and apo ptosis-related factors expression in MFC gastric cancer bearing model mice. METHODS ：MFC gastric cancer bearing model was established by inoculating MFC gastric cancer cells into the right armpit of mice. After modeling ，model mice were divided into model group ，cyclophosphamide group （positive control ，25 mg/kg）and limonin high-dose ，medium-dose and low-dose groups （100，50 and 25 mg/kg），with 10 mice in each group. Other groups were given relevant medicine intragastrically ，once a day ，for consecutive 14 days，except that model group was given 0.5％ sodium carboxymethyl cellulose intragastrically. Before administration and after last administration ，the body weight of mice was measured ；spleen，thymus and tumor tissue were taken after the last administration to calculate the spleen index，thymus index and tumor inhibition rate. The percentage of CD 4+ and CD 8+ T lymphocytes ，CD4+/CD8+ ratio were detected by flow cytometry. The expression of immune function related indexes （IL-2，IL-10，IFN-γ）in serum were detected by ELISA. RT-PCR and Western blot assay were adopted to detect relative mRNA and protein expression of apoptosis-related factors [cytochrome C （Cyt-C），Bcl-2，Bax] in tumor tissue of mice. RESULTS ：There was no significant difference in body weight among the other groups except that of cyclophosphamide group was decreased significantly ，compared with model group （P＜0.05）. Inhibitory rate of tumor were （58.16 ± 7.07）％ ，（37.09 ± 4.26）％ ，（27.30 ± 3.64）％ ，（15.13 ± 2.95）％ in cyclophosphamide group ，limonin high-dose ，medium-dose and low-dose groups. Compared with model group ，spleen index ， thymus index ，the percentages of CD 4+ and CD 8+T lymphocyte cells ，CD4+/CD8+ ratio，serum levels of IL- 2 and IL- 10，relative mRNA and protein expression of Bcl- 2 in tumor of mice in cyclophosphamide group as well as the expression of IL- 10，relative mRNA and protein expression of Bcl- 2 in limonin groups were decreased significantly （P＜0.05）. The expression of IFN-γ，relative mRNA and protein expression of Cyt-C and Bax of cyclophosphamide group as well as spleen index （except for low-dose group ）， thymus index ， the percentage of CD 4 + and CD 8 + T lymphocytes，CD4+/CD8+ ratio，the expression of IL- 2 and IFN-γ，and relative mRNA and protein expression of Cyt-C and Bax in limonin groups were increased significantly （P＜0.05）. CONCLUSIONS ：Limonin can inhibit tumor growth in MFC gastric cancer bearing model mice ，and the side effects are relatively weak. Its mechanism is related to the improvement of immune function and the induction of apoptosis.

Probing the degradation of pharmaceuticals in urine using MFC and studying their removal efficiency by UPLC-MS/MS / 药物分析学报

Nutrient recovery from source-separated human urine has attracted interest as it is rich in nitrogen and phosphorus that can be utilized as fertilizer.However,urine also contains pharmaceuticals,steroid hormones,etc.and their removal is crucial as they have detrimental effects on the environment and human health.The current study focuses on investigating the degradation of pharmaceuticals using a double-chamber microbial fuel cell (MFC).Urine was spiked with four pharmaceuticals (trimethoprim,lamivudine,levofloxacin,and estrone) at a concentration of 2 μg/mL.The MFC was operated for 7 months in batch mode with this spiked urine as feed.The degradation efficiency of the MFC was studied,for which a selective liquid chromatography-tandem mass-spectrometric method was developed for the quantitation of compounds used in the spiking experiments and was validated with a lower limit of quantification of 0.39 ng/mL.The maximum removal rate achieved was 96％± 2％.The degradation mechanism involved processes like sorption and anoxic biodegradation.The voltage curve obtained showed that the presence of pharmaceuticals had an initial negative impact on power generation along with increased organic content;however,after the reactor acclimatization,increased power output was achieved with maximum organics removal at 30 h of retention time.This work opens a new perspective for the anoxic biodegradation of pharmaceuticals and can be useful in future bioremediation studies.

Study on Antitumor Mechanism of Periplaneta americana Extract C Ⅱ-3 on MFC Tumor-bearing Mice Based on 1H-NMR Metabonomics / 中国药房

OBJECTIVE：To preliminarily study the antitumor mechanism of Periplaneta americana extract C Ⅱ-3 on MFC tumor-bearing mice. METHODS ：Balb/c mice were randomly divided into model group （normal saline 20 mL/kg）and C Ⅱ-3 group （200 mg/kg），with 6 mice in each group. MFC cell suspension （0.2 mL）was injected under the right armpit of mice. On the next day，mice were given relevant medicine intragastrically ，once a day ，for consecutive 10 d. 24 h after the last administration ，Based on the measurement of tumor size ， 1H-NMR technology combined with unsupervised PCA ，supervised PLS-DA and OPLS-DA were used to compare metabolic spectrum of liver tissue from tumor-bearing mice of 2 groups，to analyze differential metabolites and to explore the potential antitumor mechanis m of C Ⅱ -3. RESULTS ：Compared with model group ，the tumor body was significantly reduced in tumor-bearing mice of C Ⅱ-3 group. There were differences in 1H-NMR spectra between the 2 No.81960712）； groups. According to unsupervised PCA ，supervised PLS-DA and OPLS-DA ，totally six potential differential metabolites ，as glycogen （increased），pyruvate （decreased），arginine （de- creased），hydroxyproline （increased），inosine （increased） and niacinamide （increased），were identified in the liver tissue，which were mainly attributed to the metabolism of arginine ，energy and nucleic acid. CONCLUSIONS：The anti tumor effect of C Ⅱ-3 may be related to the regulation of arginine metabolism ，energy metabolism and nucleic acid metabolism.

Application of a direct current circuit to pick up and to store bioelectricity produced by microbial fuel cells / Aplicación de un circuito de corriente continua para la colección y almacenamiento de bioelectricidad producida por celdas de combustible microbianas / Aplicação de um circuito de corrente contínua para coleta e armazenamento de bioeletricidade produzida por células a combustível microbianas

Abstract Every year the demand for energy worldwide is increasing. There are some alternatives to reduce these problems, such as clean energy or renewable energy. A particular alternative is the microbial fuel cells. These cells are biochemical reactors that convert chemical energy into electricity. The present research evaluated the dairy serum to produce bioelectricity from micro fuel cells (MFC) that were constructed with low-cost materials and with isolated bacteria in anaerobic sediments, located in Ecuadorian national territory, producing maximum voltages of 0.830 V in the circuit and a maximum power density of 30 mW / m2. This low voltage was worked with 50 mL MFCs and with an output voltage of 300 mV. Under these conditions, a FLYBACK lift circuit isolated by the transformer was designed. This new circuit could increase the voltage from 30 mV to enough voltage to light a 2.5 V LED. Therefore, the energy produced by the MFC can be directly used to light a LED and to charge capacitors. This study shows that these MFCs, together with the designed circuit, could be used potentially to generate clean energy.

Resumen Cada año la demanda de energía, en todo el mundo, va en aumento. Existen algunas alternativas para reducir estos problemas, tales como las energías limpias y renovables. Una alternativa muy específica es el uso de celdas de combustible microbianas. Dichas celdas son reactores bioquímicos que convierten la energía química en electricidad. La presente investigación evaluó el suero lácteo para la producción de bioelectricidad en celdas de combustible microbianas (MFC). Estas fueron construidas con materiales de bajo costo y con bacterias aisladas en sedimentos anaeróbicos, ubicados en territorio nacional ecuatoriano, produciendo voltajes máximos de 0,830 V en el circuito y una densidad de potencia máxima de 30 mW / m2. Este bajo voltaje se trabajó con MFC de 50 mL y con un voltaje de salida de 300 mV. Bajo estas condiciones, se diseñó un circuito de elevación FLYBACK aislado por transformador. Este nuevo circuito aumentará el voltaje de 30 mV a un voltaje suficiente para encender un LED de 2.5 V. Por lo tanto, la energía producida por las MFC puede ser directamente utilizable para encender un LED y cargar los condensadores. Este estudio muestra que dichas celdas MFC, junto con el circuito diseñado, podrían utilizarse, potencialmente, para generar energía limpia.

Resumo Todos os anos a demanda por energia, em todo o mundo, está aumentando. Existem algumas alternativas para reduzir esses problemas, como energias limpas e renováveis. Uma alternativa muito específica é o uso de células combustíveis microbianas. Essas células são reatores bioquímicos que convertem energia química em eletricidade. O presente trabalho avaliou o soro lácteo para a produção de bioeletricidade em células a combustível microbianas (CCM), Estes foram construídos com materiais de baixo custo e bactérias isoladas em sedimentos anaeróbios, localizados no território nacional equatoriano, produzindo tensões máximas de 0,830 V no circuito e uma densidade de potência máxima de 30 mW / m2. Esta baixa voltagem trabalhamos com CCM de 50 mL e com uma voltagem de saída de 300 mV. Sob essas condições, um circuito de elevação FLYBACK isolado por transformador foi projetado. Este novo circuito aumentará a tensão de 30 mV para uma tensão suficiente para ligar um LED de 2,5 V. Portanto, a energia produzida pelo MFC pode ser diretamente utilizável para ligar um LED e carregar os capacitores. Este estudo mostra que essas células CCM, juntamente com o circuito projetado, poderiam ser usadas para gerar energia limpa.

Effect of Yiqi Jianpi Huayu Recipe Combined with 5-FU on Growth and Immune Function of Subcutaneous Transplanted Tumor in MFC Bearing Mice / 中国实验方剂学杂志

Objective:To discuss the effect of Yiqi Jianpi Huayu recipe (YQJPHY) combined with 5-fluorouracil(5-FU) on the growth and immune function of subcutaneous transplanted tumor in MFC tumor bearing 615 mice. Method:Twenty-four mice were inoculated subcutaneously to establish the transplanted tumor model of gastric cancer in mice, and then randomly divided into model control group, YQJPHY (20 g·kg-1)group, 5-FU (25 mg·kg-1) group and (YQJPHY+5-FU) combined group, with 6 rats in each group. After the last administration, the transplanted tumor, spleen and thymus were stripped completely. The tumor inhibition rate, thymus and spleen index were calculated. Flow cytometry was used to determine the content of myeloid-derived suppressor cells (MDSCs) and its subtype polykaryotype cells (PMN-MDSC), single karyotype cells (M-MDSC) in both peripheral blood and tumor tissue, and macrophages and their M1 type, M2 type, T lymphocyte, B lymphocyte, and natural killer cells (NK cells) in peripheral blood. Expressions of arginase-1(Arg-1) and inducible nitric oxide synthesis (iNOS) gene in tumor tissues were detected by Real-time PCR. Result:Compared with model control group, the weight of mice in YQJPHY group increased, whereas the weight of tumor, the weight of tumor, the index of thymus and spleen decreased in 5-FU group(PPPPPPPP+,CD4+,CD8+ T cell group decreased(PPPPPPConclusion:YQJPHY can better inhibit the growth of subcutaneous transplanted tumor when combined with 5-FU, and improve immune status after chemotherapy. The mechanism may be related to the decrease of MDSCs content and the increase of T cell and macrophages content.

An efficacy analysis of anti-vascular endothelial growth factor therapy for choroidal neovascularization secondary to multifocal choroiditis and comparison with wet age-related macular degeneration / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

OBJECTIVE@#To evaluate the effect of anti-vascular endothelial growth factor (VEGF) on juxtafoveal choroidal neovascularization (CNV) secondary to multifocal choroiditis (MFC) and wet age-related macular degeneration (AMD).@*METHODS@#In this retrospective, comparative study, 20 unique eyes with CNV were divided into two groups: 10 patients affected by MFC and 10 patients diagnosed with wet AMD. They all received local intravitreal (IVT) injections of ranibizumab, with 6 months of follow-up. Retreatment injections were performed based on findings suggestive of active neovascularization.@*RESULTS@#Significant improvements were observed in the juxtafoveal CNV lesions, and average central macular thickness decreased in both groups following the anti-VEGF therapy (P<0.05). The average number of injections used in MFC patients was 1.6, while three injections on average were used in wet AMD patients (Z=-2.844, P=0.009). Best-corrected visual acuity was significantly improved in MFC patients after anti-VEGF therapy (P<0.05), and there was no significant difference in wet AMD patients between before anti-VEGF therapy and 6 months later (P>0.05).@*CONCLUSIONS@#IVT ranibizumab resulted in good clinical outcomes for juxtafoveal CNV secondary to MFC and wet AMD, but the average number of injections used in MFC was fewer than that used in wet AMD over a 6-month observation period. Compared with the wet AMD group, visual acuity was obviously improved in the MFC group at 6 months.

Antimicrobial and Anticancer Activities of Ethyl Acetate Extract of Co-culture of Streptomyces sp. ANAM-5 and AIAH-10 Isolated From Mangrove Forest of Sundarbans, Bangladesh.

In this study we investigated the antimicrobial and anticancer activities of ethyl acetate extract of co-culture of Streptomyces sp. ANAM-5 and AIAH-10 isolated from soil of mangrove forest Sundarbans, Bangladesh. The antimicrobial activity of ethyl acetate extract was determined using broth-dilution method against Candida albicans, Saccharromyces cerevaceae and Aspergillus niger whereas anticancer activity was evaluated against Ehrlich Ascites Carcinoma (EAC) cells in Swiss albino mice with the dose of 50 mg/kg and 100 mg/kg body weight (i.p). The Minimum Inhibitory Concentrations (MIC) of ethyl acetate extract was found 32μg/ml against Candida albicans while 64 μg/ml against Saccharromyces cerevaceae and Aspergillus niger. The antineoplastic activity of the crude extract was increased in dose dependent manner with a significant value (p<0.01). Bacterial crude extract enhanced the mean survival time (MST) of tumor bearing mice at 71.79% and maximum cell growth inhibition was found 75.75 % with dose of 100 mg/kg body weight (i.p.). Our study revealed that ethyl acetate extract of co-culture of Streptomyces sp. ANAM-5 and AIAH-10 is an excellent source of antimicrobial and anticancer compounds which may become helpful to treat infections and cancer.

Antifungal Properties of Malaysian Tualang Honey and Stingless Bee Propolis against Candida albicans and Cryptococcus neoformans.

Candida albicans and Cryptococcus neoformans can cause life-threatening infections, especially in immune-compromised patients. Treatment with currently available antifungal agents may lead to severe side-effects and emergence of resistant strains. The objective of this study was to evaluate the antifungal properties of MTH and SBP against C. albicans and C. neoformans. Broth dilution method was used to assess the antifungal properties of the MTH and propolis. Different concentrations of the MTH and propolis (0.78 mg/mL – 50.00 mg/mL) in two-fold dilutions were tested against each fungus to determine the Minimum Inhibitory Concentration (MIC) which was done by visual inspection and spectrophotometric (MIC95) reading at 620 nm. Minimum Fungicidal Concentration (MFC) was obtained by culturing on Sabouraud Dextrose Agar. Total phenolic acids and flavonoids contents were also determined by Folin-Ciocalteu and colorimetric assay respectively. The MICs of the MTH against C. albicans and C. neoformans by visual inspection were 6.25 mg/mL and 1.56 mg/mL respectively, meanwhile 6.25 mg/mL and 3.13 mg/mL by spectrophotometric reading. The MFCs of the MTH against C. albicans and C. neoformans were 12.50 mg/mL and 6.25 mg/mL respectively. The MICs of SBP against C. albicans and C. neoformans by visual inspection were both 1.56 mg/mL whereas spectrophotometric reading recorded MICs of 3.13 mg/mL and 1.56 mg/mL respectively. The MFCs of SBP against C. albicans was 6.25 mg/mL and 3.13 mg/mL for C. neoformans. The total phenolic acids and flavonoids contents of MTH were 275.6 mg gallic acid/kg and 71.8 mg quercetin/kg respectively whereas for SBP, the phenolic acids content was 1754.2 mg gallic acid/kg and the flavonoids content was 82.6 mg quercetin/kg. MTH and SBP exhibited significant antifungal activities against C. albicans and C. neoformans. Their antifungal activities might be attributed to the high phenolic acids and flavonoids. This result suggests that MTH and SBP could potentially be used as alternative therapeutic agents against these fungi.

Comparison of the Minimum Fungicidal Concentration of Clotrimazole, Ketoconazole, Miconazole and Terbinafine Against Clinical Isolates of Dermatophytes.

Superficial fungal infections affect millions of people worldwide. Earlier most dermatophyte strains had relatively restricted geographical distribution. But currently, dermatophytosis has become one of the most common human infectious diseases worldwide. Fungal infections are common in hot and humid climate of tropical countries like India. Topical and systemic therapies are commonly used to treat dermatophyte infections.Clotrimazole is one of the most commonly used topical antifungal drugs. This study compared the minimum fungicidal concentration (MFC) of Clotrimazole with Miconazole, Ketoconazole and Terbinafine in skin dermatophytes. The study demonstrated that Clotrimazole had lower MFCs as compared to Ketoconazole and Miconazole against Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum canis. Clotrimazole had comparable MFCs versus Terbinafine against Trichophyton rubrum but it had lower MFCs against Trichophyton mentagrophytes and Microsporum canis. Thus, Clotrimazole is an effective antifungal agent for dermatophytosis even today.The efficacy of Clotrimazole even against strains with intermediate resistance or resistance to the older azole anti fungal drugs reiterate the current decisions of empirical treatment with topical Clotrimazole for the management of superficial dermatophyte infections.

Effect of Actinidia chinensis polysaccharide on apoptosis of MFC and their orthotopic transplanted tumor of gastric cancer / 中草药

Objective: To explore the effect of Actinidia chinensis polysaccharide (ACP) on apoptosis of MFC cells and their orthotopic transplanted tumor of gastric cancer and the molecular mechanism. Methods: MTT method was used to detect the inhibitory rate of ACP on MFC cell proliferation and the expression of Mcl-1, Bcl-2, Bak, and Bcl-xl protein was detected by Western blotting; The model of orthotopic transplanted tumor of gastric cancer was established with OB anastomosis adhesive stick method; Cell apoptosis in tumor tissue was detected by TUNEL method; The expression of Bcl-2 and Bax was detected by immunohistochemistry method. Results: MTT results showed that with the increasing of ACP concentration, the inhibition of ACP on MFC cell proliferation became stronger and with the prolongation of time, the inhibition was increasing. Western blotting showed that ACP would downregulate the expression of Mcl-1, Bcl-2, and Bcl-xl protein and upregulate the expression of Bak protein when the MFC cells were treated for 24 h by ACP. The animal experiment showed that compared with the model group, the average positive indexes of ACP in mid- and high-dose ACP and 5-FU positive control groups in the detection of apoptosis by TUNEL were significantly increased (P < 0.01); Meanwhile, in all ACP groups the expression of Bcl-2 could be reduced and the expression of Bax was upregulated significantly (P < 0.01). Conclusion: ACP could induce apoptosis of gastric cancer cells, downregulate the expression of Mcl-1, Bcl-2, and Bcl-xl protein, and upregulate the expression of Bak and Bax protein, prompting that the antitumor mechanism of ACP is related with the apoptosis pathway in which the Bcl-2 family proteins are involved.

Phytochemical and antimicrobial screening of crude methanolic leaf extract of Peucedanum winkleri H. Wolff.

Phytochemical investigation of the leaves of Peucedanum winkleri H. Wolff, revealed the presence of secondary metabolites. The extract from total extraction with methanol was screened for its antimicrobial activity against Staphylococcus aureus, Methicillin resistant Staphylococcus aureus, Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pyogenes, Proteus mirabilis, Candida albicans and Candida krusei using agar-well difussion method. The result indicated that the extract inhibited the growth of one or more test pathogens and were compareable with those of the standard drugs used. The minimun inhibitory concentration (MIC) ranges from 5-10 mg/ml and the minimun bactericidal/fungicidal concentration (MBC/MFC) ranges from 20-40 mg/ml. The result of the study shows justification for the use of the plant for the treatment of infectious diseases caused by these bacteria and fungi pathogens. It was concluded that P. winkleri H. Wolff could be a potential source of active antimicrobial agents and a detailed assessment of antimicrobial activity of the plant material in other solvents extract, isolation and characterization of active compounds from the most active extract is on-going.

Evaluation of Malawian Vernonia glabra (Steetz)Vatke leaf and Securidaca longepedunculata (Fresen) root extracts for antimicrobial activities.

The aqueous, ethanol and acetone crude extracts and dichloromethane, hexane, ethyl acetate and nbutanol fractions of the Securidaca longepedunculata roots and Vernonia glabra leaves were studied for their antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans using agar well diffusion method. The phytochemical presents, as well as the minimum inhibitory concentration (MIC) and minimum bacteriacidal/fungicidal concentration (MBC/MFC) of the extracts were also determined using standard methods. Results obtained indicated that Vernonia glabra leaves acetone extracts had excellent antimicrobial activity against E. coli, P. aeruginosa and ethanol extracts against S. aureus. The n-butanol fractions had the best activity against E. coli and S. aureus, dichloromethane fraction against P. aeruginosa and ethyl acetate fraction against C. albicans. For S. longepedunculate root ethanol extracts showed best activity against E. coli, acetone extract against P. aeruginosa and aqueous extract against C. albicans. The n-butanol fractions had best activity against E. coli, P. aeruginosa and C. albicans. These results verified the claims by traditional healers in Malawi that the plants extracts treats bacteria related ailments such as diarrhoea and could be a potential source for development of phytomedicine.

Microbial fuel cells demonstrate high coulombic efficiency applicable for water remediation.

Microbial fuel cells (MFCs) convert biomass into electricity by the metabolic activity of microorganisms and are also used for remediation and water treatment. Power output was compared for a dual chambered membrane MFC using either E. coli or two Yamuna river samples, Yamuna (before the Sangam region)­­—­­slow flow (sample 1) and Sangam region —fast flow (sample 2). E. coli and the two river water samples 1 and 2 gave a maximum voltage of 779, 463 and 415 mV respectively. Using E. coli the maximum power density obtained with a 100 Ω resistor was 220.66 mW/cm2 and the highest power generated 6068.41 mW. The results demonstrate E. coli, river sample 1 and river sample 2 have a comparable coulombic efficiency of 85.2, 71 and 77% respectively when using 0.4% sucrose as substrate. The decrease in chemical oxidative demand of all river water samples using MFC technology demonstrates efficient remediation of inland water.

Anti-proliferation effect of Ginseng Rh_2 on MFC cells / 吉林大学学报(医学版)

Objective To investigate the anti-proliferation effect of Ginseng Rh2(G-Rh2)on mouse MFC gastric cells and its mechanism.Methods MFC cells (1.0?109?L-1) were divided into four gourps:control group and G-Rh2(3,10,30 mg?L-1) groups.The cell viability was determined by MTT;the cell cycle was detected by flow cytometry;the protein expression of cyclin D1 and p27kip1 were observed respectively by qualitative and quantitative analysis.Results Compared with control group,the cell viabilities decreased gradually with the increasing of dose and time in G-Rh2(3,10,30 mg?L-1) groups at different time(1,2,4 h)(P

Therapy of murine gastric cancer by 5-FC/CD suicide gene system combined with HSP-PC tumor vaccine / 中国癌症杂志

Purpose:To investigate the antitumor effect of 5-fluorocytosine/cytosine deaminase (5FC/CD) suicide gene system combined with heat shock protein 70 peptide complexes(HSP70-PC) tumor vaccine.Methods:5-FC was administrated intraperitoneally after recombinant adenoviruses carrying E.coli CD gene were injected into murine tumor tissue. At the same time,mice bearing tumor were inoculated s.c. with HSP70-PC. Results:The combined therapy led to reduction in tumor growth, and complete tumor regression was observed in 70% of the mice. After the combined therapy, the tumor tissue showed more CD + 4 and CD + 8T cells infiltration, and the murine CTL activities increased significantly. Conclusions:The therapy combining 5-FC/CD system with HSP-PC tumor vaccine is very effective in murine gastric cancer, and has potential for clinical usage.

Mechanism of anti-proliferation effect of ginseng Rh_2(G-Rh_2) on mouse MFC gastric cells / 中国药理学通报

Aim To investigate the molecular mechanism of anti-proliferation effect of Ginsenoside Rh2(G-Rh2)on mouse MFC gastric cells.Methods The cell viability was determined by MTT method and the state of cell proliferation was analyzed.The morphologic changes were observed by invert microscope and fluorescence microscope(Hoechst 33258).Early apoptotic rate was detected by Annexin Ⅴ-FITC through flow cytometry.Western blot was used to detect the p-JNK and p-c-jun activity in MFC cells.Immunocytochemistry staining was used to detect caspase-3 positive cells.Results G-Rh2 could inhibit proliferation of MFC cells in a dose-and-time-dependent manner.The morphological changes of apoptosis were observed in MFC cells after G-Rh2 treatment.The apoptotic ratio was increased.The phosphorylation of c-Jun and c-Jun NH2-terminal kinase(JNK)activity increased with time within 4 h compared with that of control.The expression of caspase-3 positive cells was increased.But all the above could be partly inhibited by pre-treatment with SP600125(5 ?mol?L-1)for 2 h.Conclusion G-Rh2 can decrease cell viability by inducing apoptosis and the process may be associated with the activation of JNK transduction pathway and expression of caspase-3.