Simultaneous determination of four flavonoids in Rubus chingii by MIPs-HPLC / 中成药

AIM To establish a molecular imprinted polymers (MIPs)-HPLC method for the simultaneous content dertermination of four flavonoids in Rubus chingii Hu.METHODS Fe3O4 magnetic MIPs was added into ethyl acetate fraction solution of R.chingii to prepare mixed MIPs.The analysis of mixed MIPs methanol eluent was performed on a 30 ℃ thermostatic Diamonsil C18 column (250 mm ×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％ formic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 266 nm.RESULTS Tannins,lilsonide,quercetin and kaempferol showed good linear relationships within the ranges of 2.4-1 232.0 μg (R2 =1)、7.1-3 648.0 μg (R2 =0.999 9)、4.7-4 840.0 μg (R2 =0.999 9)、4.8-2 440.0 μg (R2 =0.999 9),whose average recoveries were 98.94％,99.33％,99.26％ and 98.67％ with the RSDs of 2.04％,1.40％,1.76％ and 1.75％,respectively.CONCLUSION This accurate and reliable method eliminates impurity interference,which can be used for the content determination of flavonoids in R.chingii.

Simultaneous determination of four flavonoids in Rubus chingii by MIPs-HPLC / 中成药

AIM To establish a molecular imprinted polymers (MIPs)-HPLC method for the simultaneous content dertermination of four flavonoids in Rubus chingii Hu.METHODS Fe3O4 magnetic MIPs was added into ethyl acetate fraction solution of R.chingii to prepare mixed MIPs.The analysis of mixed MIPs methanol eluent was performed on a 30 ℃ thermostatic Diamonsil C18 column (250 mm ×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％ formic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 266 nm.RESULTS Tannins,lilsonide,quercetin and kaempferol showed good linear relationships within the ranges of 2.4-1 232.0 μg (R2 =1)、7.1-3 648.0 μg (R2 =0.999 9)、4.7-4 840.0 μg (R2 =0.999 9)、4.8-2 440.0 μg (R2 =0.999 9),whose average recoveries were 98.94％,99.33％,99.26％ and 98.67％ with the RSDs of 2.04％,1.40％,1.76％ and 1.75％,respectively.CONCLUSION This accurate and reliable method eliminates impurity interference,which can be used for the content determination of flavonoids in R.chingii.

Utilization of the oncoscan microarray assay in cancer diagnostics

Current strategies for cancer patient management include the use of genomic and proteomic test results to help guide therapeutic selection. The need for multi-target variant analysis is highlighted by the growing number of novel therapies to treat tumors with specific profiles and the increasing recognition that cancer is an extremely heterogeneous syndrome. Microarray analysis is a powerful genomic tool that provides genome-wide genetic information that is critical for guiding cancer treatments. Unlike constitutional applications of microarray analysis which are performed on whole blood samples, microarray analysis of solid tumors is challenging because tumor tissues are typically formalin fixed and paraffin embedded (FFPE). Genomic DNA extracted from FFPE tissues can also be fragmented into small pieces and yield much lower concentrations of DNA. We validated and implemented the Affymetrix OncoScan® FFPE assay to enable genome-wide analysis from these types of samples. The Affymetrix OncoScan® assay utilizes molecular inversion probes that generate multiplexed array hybridization targets from as short as 40 base-pairs of sequence and as low as approximately 80 ng of genomic DNA. OncoScan microarray analysis provides genomic information that includes structural variations, copy number variations and SNPs in a timely and a cost-effective manner from FFPE tumor tissues (AU)

Personality Style in Patients Looking for Tooth Bleaching and Its Correlation with Treatment Satisfaction

Abstract In the last years the focus in dentistry has shifted to an "esthetic dentistry" approach, where patients are concerned about reaching a better look of their teeth. Vital tooth bleaching is a technique with immediate results, which improves the appearance and patient's self-esteem. The aim of this study was to recognize personality characteristics determined by the Millon Index of Personality Styles of participants looking for tooth bleaching and to correlate them to satisfaction with the treatment. Forty participants were included and filled out the Millon Index of Personality Styles form before treatment. Expectation about tooth bleaching was quantified from 1 to 5. Patients were treated with bleaching agent according to manufacturer's directions. One week after treatment, the patient's satisfaction was quantified from 1 to 5. Prevailing personality characteristics were determined. Expectations and satisfaction values of all patients were correlated with each of the presented personality scales by Spearman Rho test. Ten scales prevailed over their counterpart. Median of patient's expectation was 2 and satisfaction 4. Only the score of a single characteristic (extraversing) showed statistically significant correlation with patient satisfaction. Patients looking for tooth bleaching treatment seem to have common personality characteristics. Almost all of them wanted to achieve a moderate change in teeth color and the result of the treatment was usually satisfactory.

Resumo Ultimamente, o foco da Odontologia deslocou-se para uma "odontologia estética", em que os pacientes se preocupam em conseguir uma aparência melhor para seus dentes. O branqueamento vital é uma técnica com resultados imediatos que melhora aparência e auto-estima do paciente. O objetivo deste estudo foi detectar características de personalidade definidas pelo Índice Millon de Estilos de Personalidade de pessoas que buscaram branqueamento dental e correlacioná-las com a satisfação com o tratamento. Foram incluídos 40 participantes que preencheram o formulário do Índice Millon de Estilos de Personalidade antes do tratamento. A expectative sobre o branqueamento dental foi quantificada com valores de 1 a 5. Os pacientes foram tratados com agente branqueador de acordo com as instruções do fabricante. Uma semana depois o tratamento, a satisfação do paciente foi quantificada com valores de 1 a 5. Foram determinados as características predominantes. Os valores da expectativa e da satisfação foram correlacionados com cada escala de personalidade apresentada através do teste de Spearman Rho. Dez escalas prevaleceram sobre a sua contrapartida. A mediana das expectativas foi 2 e das satisfações foi 4. O valor de uma característica apenas (extraversão) apresentou correlação estatisticamente significante com a satisfação do paciente. Os pacientes que buscam o branqueamento dental parecem ter características comuns de personalidade. Quase todos queriam conseguir uma mudança moderada na cor dos dentes e o resultado do tratamento foi geralmente satisfatório.

Relaciones entre estilos de personalidad y tipos de consumo de alcohol / The Relationship between Personality Styles and Rate of Alcoholic Consumption / Relações entre estilos de pessoalidade e tipos de consumo de álcool

Objetivo. Esta investigación buscó identificar, en la población adulta del municipio de El Retiro (Antioquia), los tipos de consumo de alcohol, así como su relación con estilos de personalidad. Método. Se realizó un estudio transversal, descriptivo y correlacional. Se aplicaron dos instrumentos, Millón Inventory of Personality (MIPS) y Alcohol Use Disorder Identification Test (Audit) en una muestra de 61 participantes, de los cuales 33 fueron hombres y 28 mujeres. Resultados. El tipo de consumo de bebidas alcohólicas más frecuente en el municipio de El Retiro fue el consumo sin riesgo (68.9%,). Además, se presentaron diferencias estadísticamente significativas en el tipo de consumo de alcohol según el género. Particularmente, los hombres registraron dependencia al alcohol. Asimismo, se halló que en los hombres el consumo de alcohol se encuentra relacionado con los estilos de personalidad como la innovación (r = 0.35), la discrepancia (r = 0.40) y el dominio (r = 0.46); mientras que en las mujeres se relacionan con la discrepancia (r = 0.38), la expansión (r = 0.39), la modificación (r = 0.40) y la adecuación (r = 0.42). Para ambos géneros, el consumo de riesgo se correlaciona con el estilo de sistematización (r = 0.67), el consumo perjudicial con la protección (r = 0.89) y la dependencia al alcohol con la indecisión (r = 0.99). Conclusión. Las tres categorías en las que se agrupan los estilos de personalidad (metas motivacionales, modos cognitivos y comportamientos interpersonales), se asociaron diferencialmente con el consumo sin riesgo, con riesgo, perjudicial y dependencia, encontrando que estas relaciones entre estilo de personalidad y consumo también muestran diferencia por género.

Objective. To detect the rate of alcohol consumption and its relation to personality styles in the adult population of the town of "El Retiro" (Antioquia, Colombia). Method. The study was cross-sectional, descriptive and correlational. Two instruments were applied, the Millón Inventory of Personality (MIPS) and Alcohol Use Disorder Identification Test (Audit) in a sample of 61 participants, of which 33 were men and 28 were women. Results. The most common rate of drinking in El Retiro was Safe Consumption (68.9%). There were statistically significant differences in the rate of alcohol consumption by gender, with men in particular showing alcohol dependence. In the case of men, alcohol consumption is related to personality styles such as Innovation (r = 0.35) Discrepancy (r = 0.40), and Domain (r = 0.46) and in women Discrepancy (r = 0.38), Expansion (r = 0.39), Modification (r = 0.40) and Adequacy (r = 0.42). Consumption of risk for both genders correlated to the style of systematization (r = 0.67), harmful use with protection (r = 0.89) and alcohol dependence with indecision (r =0.99). Conclusion. The three main categories which group the personality styles of motivational goals, cognitive modes and interpersonal behaviors are differentially associated with consumption without risk, with risk, harmful and dependent, with significant gender-related differences when taking into account personality style and consumption.

Escopo. Esta pesquisa procurou identificar, na população adulta do município El Retiro (Antioqia- Colômbia), os tipos de consumo de álcool, assim como sua relação com estilos de pessoalidade. Metodologia. Para isto, foi feito um estudo transversal, descritivo e correlacional. Foram aplicados dois instrumentos, Millón Inventory of Personality (MIPS) e Alcohol Disorder Identification Test (Audit) em uma amostra de 61 participantes, dos que 33 foram homens e 28 mulheres. Resultados. O tipo de consumo de bebidas alcoólicas mais frequentes no município de El Retiro foi o consumo sem risco (68.9%). Além, apresentaram-se diferenças estatisticamente significativas no tipo de consumo segundo o gênero. Particularmente, os homens registraram dependência ao álcool. Também foi achado que nos homens o consumo de álcool esta relacionado com os estilos de pessoalidade como a inovação (r = 0.35), a discrepância (r = 0.40) e o domínio (r = 0.46), enquanto que nas mulheres estão relacionadas com a discrepância (r = 0.38), a expansão (r = 0.39), a modificação (r = 0.40) e a adequação (r = 0.42). Para ambos gêneros, o consumo de risco esta correlacionado com o estilo de sistematização (r = 0.67), o consumo prejudicial com a proteção (r = 0.89) e a dependência ao álcool com a indecisão (r = 0.99). Conclusão. As três categorías nas que estão agrupados os estilos de pessoalidade (metas motivacionais, modos cognitivos e comportamentos interpessoais), foram associados diferencialmente com o consumo sem risco, prejudicial e dependência, encontrando que estas relaciones entre estilo de pessoalidade e consumo também mostram diferencias por gênero.

Study on targeted separation of atractylenolide IQ from traditional Chinese herbs by molecularly imprinted solid phase extraction / 中国药学杂志

OBJECTIVE: To establish a novel and efficient method for enriching low content active components from traditional Chinese herbs. METHODS: The enrichment of atractylenolide III was taken as an example. First, molecular imprinting polymers (MIPs) of atractylenolide III were prepared by precipitation polymerization using 1-Vinylimidazole as functional monomer. Second, the atractylenolide III MIPs were packed into solid-phase column to separate atractylenolide III and its analogues from the exracts of Herba Atractylodes Macrocephaia and Codonopsis pilosula. Moreover, the adsorption performance of MIPs for the target components was also investigated. RESULTS: Enrichment factor (EF) of MIP-SPE and C18-SPE column were 78.90 and 51.56 μg · g-1 respectively, suggesting that MIPs had better adsorption property than C18; the precision and accuracy of the developed method were satisfactory with recoveries of 102.2% and LOD of 0.36 μg · mL-1. CONCLUSION These results demonstrate the feasibility of molecularly imprinted solid phase extraction for enriching low content active components in traditional Chinese herbs.

Perfil psicológico de los estudiantes de 1er año de enfermería: estudio preliminar / Psychological profile of 1st year nursing students: preliminary study

La presente investigación tuvo como propósito elaborar un perfil de los estudiantes de primer año de la carrera de Enfermería de la Universidad Santo Tomás, con la finalidad de obtener información sobre los diversos ámbitos del funcionamiento del alumno. Se realizó un estudio de tipo exploratorio-descriptivo, con una muestra compuesta por 71 sujetos de ambos sexos. A éstos se les aplicaron los inventarios de personalidad MIPS (Inventario Millón de Estilos de Personalidad) y el POI (Inventario de Auto-Actualización Personal). Los resultados indican que los sujetos en el MIPS manifiestan una presencia marcada de los rasgos: apertura, sensación, sistematización, firmeza, conformismo y control. Los hallazgos del POI indican que los estudiantes presentan un alto nivel de autoaprecio, y un adecuado nivel de autonomía, espontaneidad, autoaceptación y aceptación de la agresión.

The present research protocol was designed to develop a psychological profile of first year nursing students at Santo Tomás University, and therefore obtain information related to different behavioral areas. An exploratory-descriptive research protocol for 71 students of both genders was designed. Million Indexes of Personality Styles (MIPS) and Personal Orientation Inventory (POI) were used. The results of MIPS showed a marked presence of these traits: Openness, Feeling, Systematization, Strength, Conformity and Control. The POI results showed the students possessed a high level of self regard, and an adequate level of support ratio, spontaneity, self acceptance and acceptance of aggression.

Theodore Millon, una teoría de la personalidad y su patología / Theodore Millon, a theory of the personality and its pathology

Desde su obra seminal de 1976, Psicopatología moderna: un enfoque biosocial de los aprendizajes erróneos y de los disfuncionalismos, y por más de 30 años Theodore Millon ha desarrollado una ciencia compleja de la personalidad y su patología. En su modelo, el autor postula la existencia de tres polaridades básicas, placer-dolor, activo-pasivo y yo-otros, para explicar las diferencias entre los diferentes tipos de personalidad. El modelo original de aprendizaje biosocial fue reformulado en 1990 a la luz de los conceptos evolutivos. A lo largo de todo este tiempo, Millon junto a sus seguidores han establecido un paradigma para comprender la personalidad. Al mismo tiempo, há argumentado reiteradamente que una ciencia clínica comprensiva de la personalidad necesita integrar cuatro componentes básicos: teoría o esquemas conceptuales explicativos, una nosología formal, herramientas de evaluación, e intervenciones terapéuticas adecuadas. En este trabajo, se resumen los aspectos primordiales del Modelo de Millon en lo que hace a esos diferentes componentes.

From its seminal work of 1976, Psicopatología moderna: un enfoque biosocial de los aprendizajes erróneos y de los disfuncionalismos, and by more than 30 years Theodore Millon has developed a complex science of the personality and its pathology. In his model, the author postulates the existence of three basic polarities, pleasure-pain, active-passive and I-other, to explain the differences between the different types from personality. The original model of biosocial learning was reformulated in 1990 to the light of the evolutionary concepts. Throughout all this time, Millon next to their followers has established a paradigm to understand the personality. At the same time, it has argued repeatedly that a comprehensive clinical science of the personality needs to integrate four basic components: explanatory theory or conceptual schemes, a formal nosology, suitable tools of evaluation, and therapeutic interventions. In this work, the fundamental aspects of the Model of Millon are transformed in which it does to those different components.

Grupos de Orientación a padres: evaluación de resultados / Orientation groups for parents: an out-come study

Se presentan los resultados preliminares de un estudio sobre evaluación cambios en la contención emocional de padres o adultos responsables de niños entre 6 y 8 años de edad que son asistidos en grupos de orientación a padres, en el Subprograma de Psicología Clínica de Niños de la Segunda Cátedra de Psicoanálisis: Escuela Inglesa, Facultad de Psicología, U.B.A.; con sede en la Regional Sur (Partido de Avellaneda, Provincia de Buenos Aires). Objetivos del estudio: evaluar los estilos de personalidad de los adultos responsables; analizar su capacidad de contención emocional; indagar posibles modificaciones a partir de la inserción en grupos de orientación.Metodología: la muestra está compuesta por 2 grupos de orientación (19 adultos responsables). Administración del MIPS (Millon) al inicio y retest a los 6 meses. Conclusiones: se registran modificaciones en la capacidad de contención emocional en varios de los casos.