Puerarin inhibits inflammation and lipid accumulation in alcoholic liver disease through regulating MMP8 / 中国天然药物

Alcoholic liver disease (ALD) is a growing global health concern, and its early pathogenesis includes steatosis and steatohepatitis. Inhibiting lipid accumulation and inflammation is a crucial step in relieving ALD. Evidence shows that puerarin (Pue), an isoflavone isolated from Pueraria lobata, exerts cardio-protective, neuroprotective, anti-inflammatory, antioxidant activities. However, the therapeutic potential of Pue on ALD remains unknown. In the study, both the NIAAA model and ethanol (EtOH)-induced AML-12 cell were used to explore the protective effect of Pue on alcoholic liver injury in vivo and in vitro and related mechanism. The results showed that Pue (100 mg·kg-1) attenuated EtOH-induced liver injury and inhibited the levels of SREBP-1c, TNF-α, IL-6 and IL-1β, compared with silymarin (Sil, 100 mg·kg-1). In vitro results were consistent within vivo results. Mechanistically, Pue might suppress liver lipid accumulation and inflammation by regulating MMP8. In conclusion, Pue might be a promising clinical candidate for ALD treatment.

Redução de bactérias cultiváveis, lipopolissacarídeos e colagenase/mmp-8 no tratamento endodôntico associado à ativação ultrassônica na infecção endodôntica primária: estudo clínico randomizado / Cultivable bacteria, lipopolysaccharides and neutrophil collagenase/mmp-8 reduction on the endodontic treatment associated to ultrasonic activation in primary endodontic infection: a randomized clinical trial

O objetivo desse estudo clínico randomizado foi avaliar em dentes com infecção endodôntica primária (IEP) os microrganismos anaeróbios cultiváveis (UFC/mL), níveis de endotoxinas (LPS) (EU/mL) e colagenase/MMP-8 da região periapical antes e durante o tratamento endodôntico utilizando diferentes protocolos de irrigação final: irrigação convencional (CNI), ativação ultrassônica passiva (PUA) e ativação ultrassônica continua (CUA). Quarenta e cinco dentes com IEP foram submetidos ao tratamento endodôntico utilizando NaOCl 2.5% como solução irrigadora seguido do uso de EDTA 17%. Os protocolos de limpeza final foram realizados com irrigação com agulha convencional (controle), PUA e CUA; na sequência os canais foram preenchidos com pasta de Ca(OH)2 + SS por 14 dias. Foram feitas coletas do conteúdo do canal radicular após abertura coronária (S1), após diferentes protocolos de irrigação final (S2) e após uso de medicação intracanal com pasta de hidróxido de cálcio [Ca(OH)2] (S3). O conteúdo coletado do canal foi submetido as análises por cultura microbiológica (UFC/mL) e níveis de endotoxinas (EU/mL) pelo Lisado de Amebócitos de Limulus (LAL). Após preparo dos canais e após medicação com Ca(OH)2 foram realizadas coletas do fluido periapical para análise da expressão de MMP-8 por ELISA. A análise estatística dos dados foi realizada pelo testes Kruskal-Wallis e Dunn para analise intergrupos de UFC/mL, LPS e MMP-8, todos eles com nível de significância de 5% (P<0.05). Microrganismos anaeróbios cultiváveis foram detectados em 100 % das amostras iniciais (S1) com valor médio de 1.18 x 105 CFU/mL (20 ­ 1.84 x 106 CFU/mL). CUA apresentou maior efetividade na redução de microrganismos anaeróbios cultiváveis em S3 (97.27%) seguido pela PUA (96.56%) e CNI (96.2%). Endotoxinas foram identificadas em 100% das amostras em S1 com valor médio de 538.10 EU/mL (0.017 x 6190 EU/mL), CUA mostrou uma maior redução nos níveis de LPS em S3 (97.75 %), seguido pela PUA (97.11%) e CNI (90.42%). O protocolo de irrigação final com ativação ultrassônica PUA e CUA favoreceu a redução de MMP-8 em comparação ao grupo CNI. Houve correlação estatística positiva entre os níveis de LPS e MMP-8. Conclui-se que o preparo biomecânico produz a maior redução no número de microrganismos anaeróbios e endotoxinas; O protocolo de irrigação final com PUA e CUA em associação ao preparo biomecânico produz maior redução nos níveis de Endotoxinas e MMP-8 em comparação com CNI. Existe correlação estatística positiva entre os níveis de LPS e MMP-8 presentes nas infecções endodônticas primárias, mostrando a associação do LPS com o processo de destruição tecidual(AU)

The aim of this randomized clinical trial was to evaluate in cultivated anaerobic microorganisms (CFU / mL), endotoxin levels (LPS) (EU / mL) and periapical collagenase / MMP-8 in teeth with primary endodontic infection (EPI) before and during Endodontic treatment using different final irrigation protocols: conventional irrigation (CNI), passive ultrasonic activation (PUA) and continuous ultrasonic activation (CUA). Forty-five teeth with IEP were submitted to endodontic treatment using 2.5% NaOCl as an irrigating solution followed by 17% EDTA. Final cleaning protocols were performed with conventional needle irrigation (control), PUA and CUA; The root canals were then filled with Ca (OH)2 paste for 14 days. Root canal contents were collected after coronary opening (S1), after root canal preparation (S2) and after intracanal calcium hydroxide paste [Ca (OH)2] (S3). Samples of the root canal content was performed to analysis by microbiological culture (CFU / mL) and endotoxin levels (EU / mL) by Limulus Amebocyte Lysate (LAL). After canal preparation and after Ca (OH)2 medication, periapical fluid samples were collected for analysis of MMP-8 expression by ELISA. Statistical analysis of the data was performed by Kruskal-Wallis and Dunn tests for intergroup analysis of CFU / mL, LPS and MMP-8, all with a significance level of 5% (P <0.05). Cultivable anaerobic microorganisms were detected in 100% of the initial samples (S1) with a mean value of 1.18 x 105 CFU / mL (20 - 1.84 x 106 CFU / mL). CUA showed greater effectiveness in reducing anaerobic microorganisms cultivable in S3 (97.27%) followed by PUA (96.56%) and CNI (96.2%). Endotoxins were identified in 100% of samples in S1 with a mean value of 538.10 EU / mL (0.017 x 6190 EU / mL), CUA showed a greater reduction in LPS levels in S3 (97.75%), followed by PUA (97.11%). and CNI (90.42%). The final irrigation protocol with ultrasonic activation PUA and CUA favored the reduction of MMP-8 compared to the CNI group. There was a positive statistical correlation between LPS and MMP-8 levels. It is concluded that the biomechanical preparation produces the greater reduction in the number of anaerobic microorganisms and endotoxins; The final irrigation protocol with PUA and CUA in combination with biomechanical preparation produces greater reduction in Endotoxin and MMP-8 levels compared to CNI. There is a positive statistical correlation between the levels of LPS and MMP-8 present in primary endodontic infections, showing the association of LPS with the tissue destruction process(AU)

Effects of metronidazole and amoxicillin combination on MMP-1,MMP-8 and TIMP-1 level in gingival crevic-ular fluid of patients with aggressive periodontitis / 实用口腔医学杂志

40 cases(control group)with aggressive periodontitis (AgP)received scaling and root planning (SRP)and 38 cases(test group)received SRP followed by oral administration of amoxicillin plus metronidazole for 7 d.Gingival crevicular fluid samples were exam-ined for the levels of MMP-1,MMP-8 and tissue TIMP-1 by ELISA before therapy,3 and 6 months after therapy,TIMP-1 /MMP-1 and TIMP-1 /MMP-8 ratios were calculated.The levels of MMP-1 and MMP-8 were decreased in both groups (P <0.05)at 3 and 6 months after therapy.TIMP-1 /MMP-1 and TIMP-1 /MMP-8 ratios were increased in the 2 groups(P <0.05)after treatment,3 months after therapy the ratio in test group was higher than that in control group(P <0.05).

Efficacy of minocycline and iodine glycerin in treatment with chronic periodontitis / 中国生化药物杂志

Objective To observe and compare the efficacy of minocycline and iodine ointment in treatment of chronic periodontitis.Methods 82 cases with chronic periodontitis collected in our hospital from January 2012 to January 2013 were randomly divided into groups A and groups B,each had 41 cases.Basic treatment included removal of local irritation,the use of subgingival scaling and root planing clear debris,plaque,tartar,adjust occlusal trauma and food impaction,with 3% hydrogen peroxide,chlorhexidine solution rinse the periodontal pocket were used in both two groups.Group A was added minocycline,and group B were added iodine glycerin on the basis of basic treatment.The changes of periodontal clinical parameters and MMP-8, sICAM-1 in gingival crevicular fluid before and after treatment in two groups were observed and compared, and their efficacy were evaluated. Results The efficiency of group A(minocycline)was 100%,higher than 92.8% in group B(P<0.05).Clinical indicators of periodontal mitigation were alliviate and MMP-8,ICAM content in gingival crevicular fluid were reduced in both two group after treatment (P <0.05 ). Conclusion Minocycline and iodine glycerin have good efficacy in treatment of chronic periodontitis,and minocycline is more better.

Niveles de MMP-3 y MMP-8 en pacientes con periodontitis crónica antes y después del tratamiento periodontal no quirúrgico / MMP-3 and MMP-8 levels in patients with chronic periodontitis before and after nonsurgical periodontal therapy

En la periodontitis crónica (PC) se desencadenan procesos inmunoinflamatorios, donde se liberan metaloproteinasas de la matriz (MMPs), enzimas involucradas en la degradación de la matriz extracelular, las cuales pueden ser detectadas en el fluido gingival crevicular (FGC). El propósito del estudio fue determinar los niveles de MMP-3 y MMP-8 en FGC, antes y después del tratamiento periodontal no quirúrgico (TPNQ) para evaluar actividad de la enfermedad y respuesta terapéutica. Once pacientes con periodontitis crónica y 11 controles sanos fueron seleccionados. Se evaluaron los parámetros clínicos: índice gingival, índice de placa, profundidad al sondaje y pérdida de inserción; en todos los dientes de cada individuo y en seis sitios por diente. Muestras de FGC fueron tomadas de un diente por cuadrante, con profundidad de saco ≥ 4mm y pérdida de inserción ≥ 5 mm, los niveles de MMP-3 y MMP-8 fueron determinados por ELISA. Diferencias estadísticamente significativas fueron observadas entre los parámetros clínicos del grupo control y los pacientes con PC antes del tratamiento, registrándose posterior al TPNQ disminución significativa de todos los índices. Las concentraciones iniciales de MMP-3 y 8 en el grupo con PC fueron significativamente mayores a las obtenidas luego del TPNQ y en el grupo control, sin observar correlación entre parámetros clínicos y niveles de MMPs. La disminución significativa de los valores de MMP-3 y 8 en FGC de los pacientes con PC, posterior al TPNQ, indican la participación importante de estas enzimas en la degradación del tejido, y la efectividad del tratamiento periodontal para su control.

Immune-inflammatory processes are trigged in chronic periodontitis (CP), where matrix metalloproteinases (MMPs) are released and involved in the degradation of the extracellular matrix components that can be detected in the gingival crevicular fluid (GCF). The purpose of the study was to determine the levels of MMP-3 and MMP-8 in GCF, before and after nonsurgical periodontal treatment (NSPT), to evaluate disease activity and therapy response. Eleven patients with PC and eleven healthy controls were selected. Clinical measurements to evaluate gingival index (GI), plaque index (PI), probing depth (PD) and clinical attachment loss (CAL) were made in all the teeth of each individual and in six sites per tooth. GCF samples were taken from one tooth per quadrant, with a pocket depth ≥ 4 mm and a clinical attachment loss ≥ 5 mm, and the levels of MMP-3 and MMP-8 measured using an ELISA test. Statistically significant differences in clinical parameters were observed (p < 0.05) between patients with CP and control groups before the periodontal treatment, with significant decrease in all indexes after the NSPT. The initial concentrations of MMP-3 and MMP-8 were significantly higher than those obtained after the NSPT and in the control group, without observing a correlation between the clinical parameters and the levels of MMPs. Increased levels of MMP-3 and MMP-8 in the GCF of patients with PC declined significantly after NSPT, and the difference between the levels in healthy individuals and patients, suggests the important participation of these MMPs in tissue destruction in PC disease.

Matrix metalloproteinase-8 and substance P levels in root canal exudates of nonvital teeth / 대한치과보존학회지

OBJECTIVES: The aim of this study was to investigate levels of matrix metalloproteinase-8 (MMP-8) and substance P (SP) in root canal exudates during root canal treatment (RCT) of nonvital, painful teeth. MATERIALS AND METHODS: Patients scheduled for nonsurgical RCT were prospectively selected; the study was performed after obtaining informed consent from the patients and was approved by the Institutional Review Board for Clinical Research of Gangnam Severance Hospital, Yonsei University (3-2008-0118). Canal exudates samples were collected using sterilized paper points from teeth scheduled for RCT across three different time periods. MMP-8 and SP levels were measured using enzyme-linked immunosorbent assay (ELISA). Data were analyzed using a mixed model analysis and the Pearson correlation analysis (p < 0.05). RESULTS: MMP-8 and SP levels in GCF were decreased during RCT (p < 0.0001), and they showed a weak positive correlation to each other (p < 0.05). Patients'subjective pain levels and the response from percussion test were significantly related to SP level. CONCLUSIONS: This study demonstrated that periradicular inflammation endodontic origin can elevate SP and MMP-8 levels in root canal exudates. Interestingly, SP level of canal exudates showed a possibility of being used as an indicator of pain due to periapical pathosis.

Papel da MMP-8 na doença periodontal / MMP-8 E periodontal disease

As metaloproteinases da matriz (MMP) e os inibidores de metaloproteinase da matriz (TIMP) são enzimas extremamente importantes na remodelação do tecido conjuntivo durante o desenvolvimento, homeostase e cicatrização. O desequilibrio entre as MMPs ativadas e seus inibidores endógenos leva ao colapso patológico da matriz extracelular durante a doença periodontal provocando a degradação das fibras colágenas inseridas na raiz e a migração apical do epitélio com formação da bolsa periodontal. A MMP-8 destaca-se entre as MMPs predominantemente presentes no tecido gengival inflamado, fluido gengival crevicular e saliva bem como fluido sulcular e peri-implantar. O nível e grau de ativação desta enzima parecem aumentar com o aumento da atividade e gravidade da doença periodontal e diminuir em seguida ao tratamento. Diante da importância dessa enzima na evolução da doença periodontal, esse trabalho teve como objetivo desenvolver uma revisão de literatura sobre o papel da MMP- 8 e do seu principal inibidor, TIMP-1, na degradação de colágeno gengival durante a doença periodontal, destacando suas características, mecanismo de ação e inibição, expressão tecidual e níveis no fluido gengival crevicular.

Matrix metaloproteinase (MMP) and tissue inhibitor of matrix metalloproteinase (TIMP) are extremely important enzymes in connective tissue remodeling during development, homeostasis and healing. An imbalancebetween active MMPs and TIMPs create a pathological collapse of extracellular matrix during periodontal disease generating degradation of collagen fibers attached to the root surface and epithelial apical migration with pocket formation. MMP-8 is the major class among MMPs observed in inflammed gingival tissue, gingival crevicular fluid, saliva as well as dental and peri-implantar sulcular fluid. The levels and activation of this enzyme seems to increase with the activity and severity of periodontal disease, and decrease after treatment. Due to MMP-8 relevance in periodontal disease development, this paper aimed to review MMP-8 and TIMP-1 participation in gingival collagen degradation during periodontal disease, underlining the enzyme characteristics, action and inhibition mechanisms, tissular expression and gingival crevicular fluid levels.

Effect of CD14, Toll-like receptors, cytoskeletal inhibitors and NF-kappaB inhibitor on MMP-8 release from human neutrophils induced by E. coli lipopolysaccharides / 대한치주과학회지

OBJECTIVE: MMP-8 is a neutrophil enzyme and its level increases in some inflammatory diseases, including periodontal disease. We knew that the lipopolysaccharide of E.coli(E-LPS) induced MMP-8 release from human neutrophils. E-LPS is known to induce the production and release of inflammatory cytokines through CD14, Toll-like receptor(TLR). In the present study, we investigated whether MMP-8 release by E-LPS is induced via CD14-TLR pathway and the cellular mechanism of MMP-8 release in human neutrophils. MATERIAL AND METHODS: Human neutrophils were isolated from the peripheral blood of healthy donors and pre-incubated in medium containing antibodies against CD14, anti-TLR2 and anti-TLR4 or several inhibitors of microtubules and microfilaments and then incubated with E-LPS. The cells were treated TPCK and E-LPS simultaneously. The MMP-8amount in the culture medium was determined using ELISA. RESULTS: E-LPS increased MMP-8release from neutrophils and its induction was inhibited by anti- CD14 and anti-TLR4 but not by anti-TLR2 antibodies. The inhibitors of micro- tubule and microfilament polymerization significantly decreased E-LPS-induced MMP- 8release. TPCK inhibited E-LPS-induced MMP-8 release. CONCLUSION: These results suggest that MMP-8 release is induced by E-LPS via the CD14-TLR4 signal pathway in human neutrophils and may be depedent on microtubule and microfilament systems and NF-kappaB pathway.

MMP-2, MMP-8 Expression in gingival tissue of Chronic Periodontitis associated to Type 2 Diabetes Mellitus / 대한치주과학회지

No abstract available.

MMP-2, MMP-8 Expression in gingival tissue of Chronic Periodontitis associated to Type 2 Diabetes Mellitus / 대한치주과학회지

No abstract available.

Effect of sonicated extracts of Enterococcus faecalis on the production of matrix metalloproteinase-8 by human polymorphonuclear neutrophils / 대한치과보존학회지

This in vitro study monitored MMP-8 production on PMN by stimulated with the following three groups; Sonicated extracts of E. faecalis (SEF), SEF treated with Ca(OH)2 (12.5mg/ml) for 7 days, and lipopolysaccharides (LPS) of E. coli. The level of MMP-8 in each group was immediately measured by ELISA. The data were analyzed with Kruskal-Wallis test and Mann-Whitney U test. In the SEF group, the level of production of MMP-8 was higher than the negative control group in low concentration (0.05microg/ml) of SEF (p 0.05). All of the levels in E. coli LPS were increased with increasing concentrations (p 0.05).