ABSTRACT
PURPOSE: Avulsed tooth can be completely recovered, if sound periodontal ligament (PDL) of tooth is maintained. Although a lot of storage solutions have been explored for the better storage of avulsed tooth, there is a shortcoming that the preservation time is much short. On the other hand, there has been studies that (-)-epigallocatechin-3-gallate (EGCG), the most abundant polyphenol in green tea, which is related to the anti inflammatory, antioxygenic, and antibacterial effects, allows the successful preservations of tissues and cells. This study evaluated the effect of EGCG on avulsed-teeth preservation of Beagle dogs for a period of time. METHODS: The atraumatically extracted teeth of Beagle dogs were washed and preserved with 0/10/100 microM of EGCG at the time of immediate, period 1 (4 days in EGCG-contained media and additional 1 day in EGCG-free media), period 2 (8 days in EGCG-contained media and additional 2 days in EGCG-free media) and period 3 (12 days in EGCG-contained media and additional 2 days in EGCG-free media). Then, the cell viabilities of preserved teeth was calculated by dividing optical density (OD) of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay with OD of eosin assay to eliminate the measurement errors caused by the different tissue volumes. RESULTS: From the results, the immediately analyzed group presented the highest cell viability, and the rate of living cells on teeth surface decreased dependent on the preservation period. However, the 100 microM of EGCG-treated group showed statistically significant positive cell activity than EGCG-free groups throughout preservation periods. CONCLUSIONS: Our findings showed that 100 microM EGCG could maintain PDL cell viability of extracted tooth. These results suggest that although EGCG could not be a perfect additive for tooth preservation, it is able to postpone the period of tooth storage. However, further in-depth studies are required for more plausible use of EGCG.
Subject(s)
Animals , Dogs , Catechin , Cell Survival , Eosine Yellowish-(YS) , Formazans , Hand , Organ Preservation , Periodontal Ligament , Tea , Tetrazolium Salts , Thiazoles , Tooth , Tooth Avulsion , Tooth ReplantationABSTRACT
PURPOSE: Avulsed tooth can be completely recovered, if sound periodontal ligament (PDL) of tooth is maintained. Although a lot of storage solutions have been explored for the better storage of avulsed tooth, there is a shortcoming that the preservation time is much short. On the other hand, there has been studies that (-)-epigallocatechin-3-gallate (EGCG), the most abundant polyphenol in green tea, which is related to the anti inflammatory, antioxygenic, and antibacterial effects, allows the successful preservations of tissues and cells. This study evaluated the effect of EGCG on avulsed-teeth preservation of Beagle dogs for a period of time. METHODS: The atraumatically extracted teeth of Beagle dogs were washed and preserved with 0/10/100 microM of EGCG at the time of immediate, period 1 (4 days in EGCG-contained media and additional 1 day in EGCG-free media), period 2 (8 days in EGCG-contained media and additional 2 days in EGCG-free media) and period 3 (12 days in EGCG-contained media and additional 2 days in EGCG-free media). Then, the cell viabilities of preserved teeth was calculated by dividing optical density (OD) of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay with OD of eosin assay to eliminate the measurement errors caused by the different tissue volumes. RESULTS: From the results, the immediately analyzed group presented the highest cell viability, and the rate of living cells on teeth surface decreased dependent on the preservation period. However, the 100 microM of EGCG-treated group showed statistically significant positive cell activity than EGCG-free groups throughout preservation periods. CONCLUSIONS: Our findings showed that 100 microM EGCG could maintain PDL cell viability of extracted tooth. These results suggest that although EGCG could not be a perfect additive for tooth preservation, it is able to postpone the period of tooth storage. However, further in-depth studies are required for more plausible use of EGCG.
Subject(s)
Animals , Dogs , Catechin , Cell Survival , Eosine Yellowish-(YS) , Formazans , Hand , Organ Preservation , Periodontal Ligament , Tea , Tetrazolium Salts , Thiazoles , Tooth , Tooth Avulsion , Tooth ReplantationABSTRACT
Phytolacca americana es una planta que preparada bajo el método homeopático se ha usado para el manejo de enfermedades que cursan con adenopatías, sabemos que in Vitro exhibe un efecto sobre la proliferación de células mononucleares de sangre periférica, principalmente linfocitos y sobre la producción de diversas citoquinas. El objetivo de este trabajo fue la exploración del efecto de Phytolacca americana sobre la proliferación de células mononucleares de sangre periférica y sobre la producción de TNFα, en diluciones consecutivas entre la 12 CH y la 18 CH. Para la evaluación de la viabilidad celular se usó el método MTT (Bromuro de 3(4,5 dimetil-2-tiazoil)-2,5- difeniltetrazólico) y para la cuantificación de la producción de TNFα el método ELISA, se analizaron muestras de sangre periférica de 10 individuos sanos. Se encontró de manera general aumento máximo de la proliferación celular 48 horas posterior a la exposición a Phytolacca, y un aumento en la producción de TNF α para la dilución 14 CH. Concluimos que Phytolacca americana produce un aumento de la proliferación de células mononucleares de sangre periférica y que en las diluciones 13, 14 y 18 CH se estimula la producción de TNFα, sin embargo, para el resto de diluciones, el efecto varía de manera individual.
Subject(s)
Humans , Leukocytes, Mononuclear , Phytolacca americana , Cell Proliferation/drug effects , HomeopathyABSTRACT
BACKGROUND: Although reperfusion is a salvaging method for an acute myocardial infarction, the act of reperfusion itself can paradoxically result in reactive oxygen species (ROS) mediated myocyte death. Propofol has been reported to remove ROS. This study tested the hypothesis that propofol protects H9c2 cardiomyoblasts against hypoxia/reoxygenation (H/R) injury. METHODS: For the H/R group of cells, hypoxia was induced by replacing the culture medium with serum-/-glucose free Dulbecco's modified Eagle's medium (DMEM) and by exposing cells to 0.5% O2 for 24 h. Following hypoxia, the cells were reoxygenated. The medium was then replaced with fresh medium for maintenance and propofol (0, 25, 50, 250micrometer) was added to the cells (n = 3 for each concentration). In the normoxia group of cells (n = 3), cells were incubated under 21% O2 for 48 h without propofol. The MTT (3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay was performed 8 and 24 h after reoxygenation for the H/R group of cells, and 32 and 48 h for the normoxia group of cells. The results of the MTT assay were determined with an ELISA spectrometer at a wavelength of 595 nm. RESULTS: At 8 and 24 h after reoxygenation, MTT formazans in the H/R group of cells were significantly reduced as compared with the normoxia group of cells (P < 0.05). In the presence of 25, 50 and 250micrometer propofol, the MTT activity at 8 and 24 h after reoxygenation was diminished when compared to exposure to 0micrometer propofol (P < 0.05). However, the formazans produced when cells were exposed to a concentration of 25micrometer in propofol convalesced to the level of 0micrometer propofol at 24 h after reoxygenation. CONCLUSIONS: These results suggest that propofol may play a role in increasing the number of non-viable cells during reoxygenation of the heart.