ABSTRACT
Objective To investigate the effects of all-trans-retinoic acid(atRA) on proliferation and apoptosis of smooth muscle cell in human saphenous vein organ culture.Methods Thirty segments(1cm) of human saphenous vein were divided into 3 groups: HSV group,atRA group and control group.The human saphenous vein segments in atRA group and control group were maintained in organ culture for 14 days with 100?M atRA and equivalent concentrations of pure ethanol respectively.All 30 segments human saphenous vein were harvested and the level of proliferating cell nuclear antigen(PCNA) was detected immunohistochemically.The smooth muscle cell proliferation was observed pathologically.Results Cultured saphenous vein segments developed significant neointimal formation and marked smooth muscle cell proliferation(P
ABSTRACT
Objective To explore the possibility of Lipi Huatan Formula (LHF) in inhibit ing the occurrence of early atherosclerosis and its mechanism. Methods Twent y_four rabbits were randomly allocated to Group A (normal control), Group B (mod el control) and Group C (treated with LHF). Rabbit models with early dietary ath erosclerosis were induced by the feeding of fatty food. The morphological featur es and relative thickness of aortic intima, ki_67 expression of vascular smooth muscle cells (VSMCs) and the positive ratio of apoptotic VSMCs were observed by mo rphological, immunohistochemical and TUNEL techniques. Results The relative thickness of aortic intima, ki_67 expression of VSMCs and the positive ratio o f apoptotic VSMCs were decreased in Group C as compared with Group B (P