ABSTRACT
Objective To identify the MYB transcription factors that may regulate the biosynthesis of triterpenoids and phenolic acids in Prunella vulgaris. Methods MYB transcription factor were identified from the P. vulgaris transcriptome database, their motif, physical and chemical properties, functional annotation, family evolution and expression patterns were examined. Results A total of 27 MYB transcription factors were identified. c32045.graph_c0 might inhibit the biosynthesis of rosmarinic acid by inhibiting the expression of cinnamic acid 4-hydroxylase gene, thus acting as a transcription suppressor to negatively regulate the biosynthesis of phenolic acids. c26895.graph_c0 might inhibit the biosynthesis of triterpenoids and phenolic acids by regulating the flow of metabolic intermediates in the biosynthesis of triterpenoids and rosmarinic acids. Conclusion The candidate MYB transcription factors related to the biosynthesis of triterpenoids and phenolic acids were obtained. It also laid a foundation for the further study of MYB in regulating of secondary metabolites in P. vulgaris.
ABSTRACT
Known as "the king of the five grains",tartary buckwheat (Fagopyrum tataricum) is a medicinal and edible plant with both nutritional and healthcare functions. It contains rich flavonoids, such as rutin,with balanced amino acid composition and multiple effects, like blood fat-lowering,blood giucose-lowering,blood pressure-lowering,anti-oxidation,anti-aging,anti-cancer,anti-cancer,and microcirculation-improving. Transcription factors play important roles in plant growth and development by regulating gene expressions. MYB family is one of the largest transcription factor families in plant,contains the MYB domain,and can be divided into four subfamilies:1R-MYB,R2R3-MYB,3R-MYB,4R-MYB. This family plays various roles in plant growth,plant development and flavonoid biosynthesis of secondary metabolism. In this study,the reported MYB transcription factors in F. tataricum were summarized and systemically clustered,and their interrelationships were defined to provide references for further exploring and cloning MYB transcription factors in F. tataricum. In addition,this study reviewed their regulatory functions of MYB transcription factors in flavonoid biosynthesis pathway,plant hormones pathways and other abiotic stress pathways,and made a conclusion and advances about the future research in F. tataricum. Therefore,this study will provide valuable scientific references for the functional studies of MYB family transcription factors in F. tataricum and its molecular breeding for high-quality varieties.