Two-Dimensional Geometric Morphometric Method on Frontal Sinus for Race Estimation: A Lateral Skull Radiograph Study

@#Introduction: Race estimation of unknown individual is essential in forensic investigation. The resiliency of frontal sinus makes it a potential tool for biological profiling, particularly in cases where fragmented skeleton persists. Geometric morphometrics is an efficient way to characterise shape. However, the use of frontal sinus to identify race of Malaysians is yet to be investigated. This research employed a two-dimensional (2D) geometric morphometric to examine the morphological differences of the frontal sinus among the major races in Malaysia. Methods: Lateral skull radiographs which comprising of 453 adult Malaysian (151 Malays, Chinese and Indian respectively) were used. The 2D landmarks of eight were placed on the digitalized radiographs and 2D geometric morphometric analysis was performed using MorphoJ software. Results: Procrustes ANOVA revealed a significantly different frontal sinus shape (p-value < 0.05) between races. Canonical variate analysis showed significantly different frontal sinus morphology (p-value < 0.05) between Malay and Indian as well as Chinese and Indian. Discriminant function analysis with cross-validation demonstrated a 57.4% accuracy rate. Conclusion: This population-specific study based on frontal sinus of Malaysians using the 2D geometric morphometric, though less reliable, sheds new light on the potential applicability of this method for race estimation purpose.

Development of a Valid and Reliable Scale to Assess Knowledge, Attitude and Practice (KAP) on Frailty, Nutrition and Exercise among Malaysian Elderly

@#Introduction: “Frailty Intervention through Nutrition Education and Exercise (FINE)” program is an educational program, an initiative to ameliorate frailty status among elderly due to the alarming number of frailty cases in Malaysia. The current study aims to develop and determine the validity and reliability of the KAP questionnaire on frailty, nutrition and exercise to assess the effectiveness of the “FINE” program among the Malaysian elderly. Methods: The KAP questionnaire was created based on the developed frailty module and education materials. Content and face validity were conducted before the reliability study among five health professionals and 20 elderly, while 79 elderly were involved in a reliability study in three different Projek Perumahan Rakyat (PPR) flats in the Kuala Lumpur area. Data were analysed to determine its internal consistency reliability. Results: Six items were removed during content and face validity, two from each domain. All items in the knowledge section were within an acceptable range of difficulty and discrimination following the item analysis. Yet, item-to-total correlation removes one item for attitude and four items for the practice domain. The analysis found that the internal consistency reliability was 0.852, 0.732 and 0.600 for the KAP section, respectively. Conclusion: The final version of the KAP questionnaire consisted of (11) knowledge, nine (9) attitudes and six (6) practice items proven to be valid and reliable. Thus, it could be used to assess the effectiveness of the “FINE” program among the Malaysian elderly.

Characteristics of dietary intakes including NOVA foods among pre-adolescents living in urban Kuala Lumpur – Findings from the PREBONE-Kids study

@#Introduction: Evidence showed considerable variability of health risk factors within different socioeconomic groups. This study aimed to characterise dietary intakes by total household income among a sample of Malaysian pre-adolescents in urban Kuala Lumpur. Methods: Baseline data of 243 healthy, pre-adolescent children between 9 and 11 years old including socio-demographic background (gender, ethnicity, and total household monthly income), anthropometry (body weight and height), and 7-day diet histories were collected. Secondary analysis was performed on dietary intakes to quantify food groups based on the Malaysian Dietary Guidelines and NOVA classification systems besides nutrients. Differences and associations between total monthly household income categories with anthropometry and dietary intakes were tested using independent t-test/Mann-Whitney U (depending on normality) and chi-square tests, respectively. Results: Most children in this study population had dietary intakes below the recommended serving sizes for five food groups, except meat/poultry (195.2±107.2%) and fish (110.1±106.3%) and consumed about 32% of energy from ultra-processed foods (NOVA food group 4). While there was no difference in dietary intake between the bottom 40% with the middle 40% and high 20% household income groups, the percentage of energy contributed by NOVA food group 4 (processed fats/oils, condiments, and sauces) was higher in the bottom 40% households (p=0.024). Conclusion: Most pre-adolescent children in this study, regardless of household income, did not meet dietary recommendations and ate diets comprised of less nutritious foods. Comprehensive approaches that aim to improve dietary patterns and reduce the risk of diet-related chronic diseases are warranted.

Language Intervention for Late Talkers: The Experiences of Speech-Language Pathologists in Malaysia (Intervensi Bahasa untuk Kanak-kanak Lambat Bercakap: Pengalaman Terapis Pertuturan-Bahasa di Malaysia) / Jurnal Sains Kesihatan Malaysia

@#A few studies have investigated the practise patterns of Malaysian speech-language pathologists (SLPs) when working with children with speech and language problems whose language intervention is necessary. However, these studies did not provide in-depth information about the SLPs' experiences as the studies were conducted quantitatively. Moreover, none of the studies focus on late-talkers (LTs), in which intervention for them can vary along a continuum from a wait/ watch-and-see approach to direct intervention. Thus, this study aimed to explore SLPs’ experiences in managing LTs in Malaysia. Twelve SLPs participated in individual, semi-structured phone interviews. They were asked about their experiences in managing LTs. The interviews were recorded, transcribed, and a content analysis was performed. Two themes were identified; a) practises of SLPs and b) challenges faced by SLPs in managing LT cases. The practises of SLPs included conducting assessments, developing intervention plans, providing intervention, sharing information, giving homework, providing support, and managing appointments. The challenges faced were related to parents, other individuals close to LTs, LTs themselves, and resources. Although the SLPs’ practises aligned with the international standard of speech-language pathology service delivery, there is room for improvement, especially in collaborating with parents, as one-to-one direct interventions for LTs might not be suitable due to the challenges related to resources. In addition, a few suggestions related to centred-based care and trusted social media pages can be provided to parents to assist them outside therapy sessions besides initiating the development of awareness programs and information resources for parents and other caregivers.

Patient characteristics, disease burden, treatment patterns and outcomes in patients with acromegaly: Real-world evidence from the Malaysian acromegaly registry / Journal of the ASEAN Federation of Endocrine Societies

Objective@#This study aims to report the demographic features of patients with acromegaly the disease burden, and the corresponding treatment patterns and outcomes in Malaysia.@*Methodology@#This is a retrospective study that included patients from the Malaysian Acromegaly registry who were diagnosed with acromegaly from 1970 onwards. Data collected included patient demographics, clinical manifestations of acromegaly, biochemical results and imaging findings. Information regarding treatment modalities and their outcomes was also obtained.@*Results@#Registry data was collected from 2013 to 2016 and included 140 patients with acromegaly from 12 participating hospitals. Median disease duration was 5.5 years (range 1.0 – 41.0 years). Most patients had macroadenoma (67%), while 15% were diagnosed with microadenoma. Hypertension (49.3%), diabetes (37.1%) and hypopituitarism (27.9%) were the most common co-morbidities for patients with acromegaly. Majority of patients had surgical intervention as primary treatment (65.9%) while 20.7% were treated medically, mainly with dopamine agonists (18.5%). Most patients had inadequate disease control after first-line treatment regardless of treatment modality (79.4%).@*Conclusion@#This registry study provides epidemiological data on patients with acromegaly in Malaysia and serves as an initial step for further population-based studies.

The Socio-economic and Psychological Impact of COVID-19 Pandemic on Malaysian Private Dentists: An Exploratory Study

@#The global COVID-19 Pandemic combined with nationwide economic depression and public fear had caused dental clinics in Malaysia to experience a significant reduction in patient visits with a potential impact on the socio-economic and psychological status of Malaysian private dentists. This study assesses the socio-economic and psychological impacts of the COVID-19 pandemic on Malaysian private dentists. A cross-sectional online questionnaire included demographic profile, socioeconomic factors and psychological status was used. Participation was voluntary. Descriptive statistics on the three variables were generated. Pearson Chi-square test was used to check for significant difference (p<0.05). In all, 95 private dentists voluntarily participated. Male and female participants were equally represented, mostly in the 22-25 group (62%). The Job Insecurity Scale measurement showed 58% and 48.5% participants respectively, were socio-economically and psychologically affected. The Financial Threat Scale showed 44.4% of respondents were financially affected, while 51% (measured by the General health questionnaire) displayed affected health. The socio-economicpsychological aspect was found to be statistically significant (p =0.018). Within the limitation of sample size, participating private dentists were found to be socio-economically and psychologically affected by the COVID19 pandemic.

Malaysian Herbs in Contraception: Public Perception (Herba Malaysia dalam Kontrasepsi: Persepsi Masyarakat) / Jurnal Sains Kesihatan Malaysia

@#Medicinal plants have been used in Malaysia for a long time ago. These plants have been marketed as herbal product and used in the traditional healthcare system because of its positive therapeutic effects. This paper discusses particularly several types of Malaysian herbs that are traditionally used for contraception and scientific studies related to its pharmaceutical properties showing its use among the public for its anti-fertility effects. Even though several methods of contraception have been promoted for family planning, yet, the perception of the public on the usage of synthetic steroidal contraceptives due to its serious adverse effects has made them focus on indigenous plants. Contraceptives drug-containing oestrogen and progesterone have proven to be effective and popular, However, the side effects of these drugs have sparked the idea of scientists to develop newer molecules from medicinal plants. Therefore, it is necessary to investigate in-depth qualitative research on conceptions and concerns about traditional contraceptive methods using herbal ingredients among Malaysians.

Antimicrobial Activity of Malaysian Apis mellifera Propolis against Propionibacterium acnes

@#Introduction: Acne vulgaris is a common skin disease that affects people all over the world. One of the main pathogenesis of acne is Propionibacterium acnes (P. acnes) proliferation. Propolis has long been used in folk medicine as a natural remedy. Its antimicrobial properties have all been studied extensively. However, there have been few studies on its use in acne. Thus, the goal of this study was to assess the antimicrobial potential of ethanolic (EEP) and water extracts (WEP) of Malaysian Apis mellifera propolis against P. acnes. Methods: Propolis samples were collected from Acacia mangium apiary from northern and southern regions of Peninsular Malaysia. The propolis extracts were screened for antimicrobial activity against P. acnes using an agar well diffusion assay. The minimum inhibitory concentrations (MICs) of the extracts were determined using a resazurin broth microdilution assay. Results: The antimicrobial screening demonstrated all extracts had antimicrobial activity against P. acnes. The inhibition zones at concentration 20 mg/ml were in the range of 16 mm to 24 mm which was greater than positive control (10% benzoyl peroxide) (15 mm). The EEP from northern region showed the lowest MIC values (0.32 µg/ml), followed by EEP from southern region (0.63 µg/ml), WEP from southern region (625 µg/ml) and WEP from northern region (2500 µg/ml). Conclusion: The Malaysian EEP demonstrated promising antimicrobial properties against P. acnes. Further study is needed to determine the active constituents and their possible inhibitory mechanisms against P. acnes.

Microbiome analysis of gut bacterial communities of healthy and diseased Malaysian mahseer (Tor tambroides) using 16S rRNA metagenomics approach

Aims@#The gut microbiota is referred to as an ‘extra organ’ and is critical in assisting the host in terms of nutrition and immunity. Environmental stressors could alter the gut microbial community and cause gut inflammation. This study aimed to investigate and compare the gut microbiota community between healthy and diseased Tor tambroides.@*Methodology and results@#In this study, such gut microbial alterations were explored using NGS-based 16S rDNA targeted sequencing on the Malaysian mahseer (T. tambroides). Three healthy adult and three diseased adult Malaysian mahseers (showing signs of exophthalmia, coelomic distension and petechial haemorrhage) were obtained from LTT Aquaculture Sdn Bhd. Our results revealed significant differences in microbial diversity, composition and function between both populations of T. tambroides. Alpha diversity analysis depicts lower diversity of gut microbiota composition in diseased T. tambroides as compared to the healthy group. In particular, Enterobacteriaceae, Aeromonas, Bacteroides, Vibrio and Pseudomonas were found within gut microbiota of the diseased fishes. In addition, cellulosedegrading bacteria and protease-producing bacteria were identified from the gut of T. tambroides.@*Conclusion, significance and impact of study@#Thus, our findings emphasized on the association between the alteration in gut microbiota composition and infectious abdominal dropsy (IAD) in T. tambroides. This finding is important to provide basic information for further diagnosis, prevention and treatment of intestinal diseases in fish.

Barreiras à atividade física percebidas entre adultos da Malásia durante a pandemia de COVID-19: um estudo transversal / Perceived barriers to physical activity among Malaysian adults during COVID-19 pandemic: a cross-sectional study

INTRODUÇÃO: A pandemia COVID-19 trouxe um impacto relativamente significativo no mundo. A Malásia implementou uma estratégia de isolamento social em todo o país para nivelar a curva da epidemia. À medida que os limites ao movimento e à interação social entraram em vigor, mais indivíduos parecem menos ativos fisicamente. OBJETIVO: determinar as barreiras percebidas para a atividade física entre jovens adultos da Malásia durante a pandemia de COVID-19. MÉTODOS E MATERIAIS: Total de 217 participantes incluídos na faixa etária de 18 a 40 anos participaram voluntariamente deste estudo. Os participantes foram excluídos se não fossem malaios e apresentassem quaisquer problemas psicológicos e deficiência física que impedissem a atividade física. O questionário Barrier to Being Active (BBAQ) foi distribuído por várias plataformas de mídia social de janeiro de 2021 a maio de 2021. Os dados categóricos foram apresentados usando frequência simples e absoluta na distribuição de perfis sociodemográficos e respostas do BBAQ. Qui-quadrado de Pearson com nível de confiança de 95%, p <0,05 foi utilizado como nível de significância para analisar a associação entre gênero e barreiras. RESULTADOS: A barreira relatada pelos participantes foi "falta de força de vontade" 136 (62,7%), seguida de "falta de energia" 117 (53,9%), "falta de recursos" 113 (52,1%), "falta de tempo", 109 (50,2%), "influência social" 100 (46,1%), "falta de habilidade" 48 (22,1%) e "medo de se machucar" 40 (18,4%). Não houve relação significativa entre gênero e barreira à atividade física entre jovens adultos da Malásia. CONCLUSÃO: A "falta de energia", "falta de força de vontade" e "falta de recursos" foram as barreiras mais percebidas para a atividade física entre jovens adultos da Malásia durante o confinamento por causa da COVID-19. Não houve associação significativa entre as barreiras de atividade física e o gênero.

INTRODUCTION: The COVID-19 pandemic has had a relatively significant impact on the world. Malaysia implemented a countrywide social isolation strategy to flatten the epidemic curve. As limits on movement and social interaction have come into force, more individuals appear less physically active. OBJECTIVE: To determine the perceived barriers to physical activity among Malaysian young adults during the COVID-19 Pandemic. METHODS AND MATERIALS: A total of 217 participants included with age group from 18-40 years old voluntarily participated in this study. Participants were excluded if they were non-Malaysian and had any psychological problems and physical impairment that hindered physical activity. The barrier to Being Active quiz (BBAQ) questionnaire was circulated through various social media platforms from January 2021 to May 2021. Categorical data were presented using simple and absolute frequency on the distribution of sociodemographic profiles and BBAQ responses. Pearson Chi-square with 95% confidence level, p<0.05, was used as the significant level to analyze the association between gender and barriers. RESULTS: The barrier reported by the participants was "lack of willpower" 136 (62.7%), followed by "lack of energy" 117(53.9%), "lack of resource" 113(52.1%), "Lack of time," 109(50.2%), "social influence" 100(46.1%), "lack of skill" 48(22.1%) and "fear of injury" 40(18.4%). There was no significant relationship between gender and Barrier to Physical Activity among Malaysian young adults. CONCLUSION: The "lack of energy," "lack of willpower," and "lack of resource" were the most perceived barriers to physical activity among Malaysian young adults during COVID-19 lockdown, and there was no significant association between physical activity barriers and gender.

Comparison of anthropometric profile, bone-related parameters, biochemical parameters, functional capacity, and vitamin D status – A study on postmenopausal Malaysian women

@#Introduction: The optimal level of total 25(OH)D in Malaysian postmenopausal women that is necessary to ameliorate the combined effects of decreased bone mineral and muscle mass, and increase in adiposity, as seen in osteosarcopenic obesity, is unknown. Given that these are common pathologies existing in bone, muscle and excess fat-related disorders, this study investigated the association between body composition, functional capacity and total 25(OH)D levels. Methods: This was a cross-sectional study involving 117 postmenopausal Malaysian women aged 50-88 years. Total 25(OH)D was analysed by chemiluminescent microparticle immunoassay CMIA on Siemens® platforms. Bone mineral was measured by broadband ultrasound attenuation. Functional performance was assessed using modified components of short physical performance battery test. Statistical analysis was performed using the Statistical Package for Social Sciences (SPSS Version 25.0). Results: Overall, 47% women were vitamin D deficient; 3% had none, 22% had one, 65% had two, and 10% had all three adverse body composition indicators (osteosarcopenic obesity). Individuals without any adverse body composition indicator had higher total 25(OH) D (74.7±7.0nmol/L, p=0.004) compared to all the other groups [1 (53.0±3.8nmol/L); 2 (48.7±1.9nmol/L) and 3 (58.3±5.4nmol/L)]. The single inflection point (ROC curve) for total 25(OH)D in predicting osteosarcopenic obesity was 58.9nmol/L. Conclusion: Higher vitamin D levels were associated with lower body fat, enhanced bone quality, and improved functional capacity. Maintaining vitamin D levels above >58.9nmol/L may protect Malaysian postmenopausal women against the combined pathologies of bone, muscle and excess fat.

Optimisation of topical antibacterial preparation from Malaysian kelulut honey by using xanthan gum as polymeric agent

@#The study aims to formulate and optimise topical antibacterial preparation by using Malaysian kelulut honey as the active ingredient and xanthan gum as the polymeric agent. Response surface methodology was used to optimise the preparation. The acidity, honey concentration and xanthan gum concentration were the independent variables. The zone of inhibitions on S. aureus ATCC6538 and E. coli ATCC8739 were the response variables. The optimal preparation was evaluated on its physicochemical properties, viscosity, antibacterial efficacy and stability. The antibacterial efficacy of the optimal preparation was compared to the commercially antibacterial gel (MediHoney™, Comvita). The optimal preparation was formulated at pH of 3.5, honey concentration of 90% (w/v) and xanthan gum concentration of 1.5% (w/v) with the inhibition zones measured on S. aureus ATCC6538 was 16.2 mm and E. coli ATCC8739 was 15.8 mm respectively. The factors of acidity and honey concentration have significantly influenced the inhibition zone on S. aureus ATCC6538 and E. coli ATCC8739. The utilisation of xanthan gum as the polymeric agent was fit for the preparation which showed by adequate physicochemical properties and retained of the antibacterial effects. This was supported by constant viscosity and efficacy of the preparation within the six months of stability study indicating stable and reliable preparation. Xanthan gum is a potential polymeric agent due to its effective use in preparing stable preparation with effective antibacterial properties.

Price Elasticity Of Demand And The Impact Of Taxing Sugar-Sweetened Beverages In Malaysia

@#The Malaysian government implemented an excise tax of MYR 0.40 per L on sugar-sweetened beverages in July 2019. Excise tax is imposed on sweetened drinks containing more than 5 g of sugar per 100 ml, flavoured Ultra High Temperature milk-based drinks, and fruit juices with more than 7 g and 12 g of sugar per 100 ml, respectively. We analysed the impact of excise tax on the consumption of SSBs by developing a demand model for SSBs to estimate the elasticity of demand using a two-way fixed-effect model. The tax increased the price of 1 L SSBs by 8.33%, and we estimated it to decrease the consumption of SSBs by 9.25%. The estimated own-price elasticity of demand for SSBs was −1.11 (95% CI: −1.97 to −0.25). Price of SSB is a determinant for SSB demand, but income or the price of milk are not. The estimated excise revenue calculated was MYR 357.61 million. However, industry responses via product reformulation and pass-through rates could reduce revenue and enhance or reduce health impacts.

Digital Approach for Lip Prints Analysis in Malaysian Malay population (Klang Valley): Scanning Technique (Analisis Cap Bibir Populasi Melayu di Lembah Klang secara Digital: Teknik Imbasan) / Jurnal Sains Kesihatan Malaysia

@#Lip print is useful in forensic investigations for individual identification. The present study aimed to discriminate sex based on lip print patterns in Malaysian Malay population in Klang Valley using a scanning technique. Lip prints of 360 subjects (180 males and 180 females) were taken using the lipstick-cellophane tape technique, pasted onto A4 papers. These papers were then scanned using Brother DCP-J100 printer (300dpi resolution). The images were analysed using a software based on Suzuki and Tsuchihashi’s classification. The lip print images were divided into six sections which are upper left, upper middle, upper right, lower right, lower middle and lower left. The Pearson chi-square test showed that there are significant differences (p < 0.05) between sexes in each section except for the upper middle section. In males, type V (irregular pattern) was the dominant pattern for upper left, upper right, lower right and lower left sections (ranging from 71.1% to 86.7%) while type IV (reticular pattern) was dominant at upper and middle sections (ranging from 57.2% to 66.1%). Type IV (48.3%) was dominant in upper middle section for female, while for the rest of the sections, type V was the dominant lip print pattern (ranging from 40% to 82.8%). These results showed that sex differentiation between males and females in Malaysian Malay population, Klang Valley can be suggested to be used for identification in forensic investigation.

Discordance between Fasting Plasma Glucose (FPG) and HbA1c in Diagnosing Diabetes and Pre-diabetes in The Malaysian Cohort / Journal of the ASEAN Federation of Endocrine Societies

Objective@#In this present study, we aim to evaluate the accuracy of the HbA1c relative to fasting plasma glucose (FPG) in the diagnosis of diabetes and pre-diabetes among The Malaysian Cohort (TMC) participants.@*Methodology@#FPG and HbA1c were taken from 40,667 eligible TMC participants that have no previous history of diabetes, aged between 35-70 years and were recruited from 2006 – 2012. Participants were classified as normal, diabetes and pre-diabetes based on the 2006 World Health Organization (WHO) criteria. Statistical analyses were performed using ANOVA and Chi-square test, while Pearson correlation and Cohen’s kappa were used to examine the concordance rate between FPG and HbA1c.@*Results@#The study samples consisted of 16,224 men and 24,443 women. The prevalence of diabetes among the participants was 5.7% and 7.5% according to the FPG and HbA1c level, respectively. Based on FPG, 10.6% of the participants had pre-diabetes but this increased to 14.2% based on HbA1c (r=0.86; P<0.001). HbA1c had a sensitivity of 58.20 (95% CI: 56.43, 59.96) and a specificity of 98.59 (95% CI: 98.46, 98.70).@*Conclusion@#A higher prevalence of pre-diabetes and diabetes was observed when using HbA1c as a diagnosis tool, suggesting that it could possibly be more useful for early detection. However, given that HbA1c may also have lower sensitivity and higher false positive rate, several diagnostic criteria should be used to diagnose diabetes accurately.

Validation of the Malay Version of the Fear of Progression Questionnaire-Short Form (FoP-Q-SF-M) in Malaysian Cancer Patients

@#Introduction: Fear of cancer progression may lead to worsening of psychological complications of cancer and affects the quality of life of cancer patients. Hence, fear of cancer progression needs to be monitored. This study translated the original English version of the Fear of Progression Questionnaire-Short Form (FoP-Q-SF) into the Malay language and investigated the reliability and validity of the Malay version of the FoP-Q-SF (FoP-Q-SF-M) among Malaysian cancer patients. Methods: Concurrent translation and back translation of the English version of the FoP-Q-SF to the FoP-Q-SF-M was performed, and the FoP-Q-SF-M and the Malay version of the Cancer Therapy Satisfaction Questionnaire (CTSQ-M) (as a comparison to assess the discriminant validity of the FoP-Q-SF-M) were administered to 200 cancer patients with different sites, stages, and duration of cancer diagnosis. Results: The CTSQ-M exhibited excellent internal consistency (Cronbach’s α= 0.927) as well as demonstrated good convergent and discriminant validity. Exploratory factor analysis of the FoP-Q-SF-M extracted one factor with twelfth items, thereby this supports the single-factor model reported by the English version of the FoP-Q-SF. Conclusion: The FoP-Q-SF-M exhibited acceptable psychometric properties and valid for use to assess fear of cancer progression in the Malaysian cancer population.

Prevalence and factors associated with smoking cessation among elderly in Malaysia- A findings from the population-based study

@#Introduction The information of prevalence and associated factor(s) with smoking cessation among the elderly will assist in formulating the suitable policies and reducing the mortality among Malaysian elderly due to smoking-related diseases. This paper aims to describe the prevalence and factors associated with smoking cessation among Malaysian elderly. Methods We carried out the population-based study using the cross-sectional study design and a sample size of 2,674 respondents obtained via a multistage sampling method. Validated questionnaire and face-to-face interviews approach were the methodologies employed to collect data from the selected respondents. The interview was conducted by trained enumerator using a standardized and validated questionnaire. Descriptive and multiple logistic regression were used in data analysis. Results The present findings demonstrated that almost 1 out of 10 of Malaysian elderly are current smokers. The prevalence of smoking was significantly higher among men (28.1%) compared to women (2.9%). Multivariable analysis showed that females (AOR 2.33, 95 CI 1.66-4.55) and those with advancing age were more likely to stop smoking compared to the younger-aged elderly (70-74 year, AOR 2.38(95 CI 1.24-4.53), 75 year and above, AOR 2.82, (95CI, 1.57-5.05. 60-64 years as reference), whiles other ethnicities (AOR 0.11, 95 CI 0.02-0.61) were less likely to cease smoking compared to elderly from Chinese descent. Based on multivariate analysis, diabetes, hypertension and hypercholesterolemia were not significant factors associated with smoking cessation. Conclusions The study has shown a low proportion of Malaysian elderly is ex- smoker. Suitable measures and programmes should be formulated and implemented to focus on the elderly groups identified in this study, to increase the quit smoking rate among them and subsequently to increase the quality of life by reducing the burden of smoking-related disease among the Malaysian elderly. Keywords Malaysian elderly - smoking cessation - chronic diseases.

Appraisal of wellness-lifestyle status among Malaysian undergraduate students: A cross-sectional and gender-based wellness survey

Wellness is a continuous and intentional attempt to remain healthy and attain a maximum potential for well-being.A high level of wellness is vital to produce a well-balanced and competent graduate. This study aims at evaluatingthe wellness status of Malaysian undergraduate students. A total of 250 undergraduate students drawn from variousfaculties in one of the public universities in Malaysia participated in this study from 2019 to 2020. The wellnesslifestyle inventory that evaluates an initial rating of a person’s present attempt to remain healthy was used as a tool forassessing the wellness status of the students. Analysis for the rating scores in their awareness revealed that the studentshave an excellent to moderate awareness on key wellness elements consisting of avoiding chemical dependency,personal safety, emotional well-being, environmental health and protection, stress management, personal hygiene,and disease prevention, while poor scores were recorded in nutrition and health-related fitness. With reference togender, it was observed that female students were significantly more informed in the four major areas of wellnesselements, specifically health-related fitness, personal hygiene and health, disease prevention, and environmental healthprotection, while male students were better at avoiding chemical dependency (p < 0.05). The findings of this studyprovide significant information on the wellness-lifestyle status of the students and its practices that could assist thestakeholders to be informed about the students’ overall wellness, in order to be able to design appropriate activitiesand programs to cater to their needs.

Sexual Dimorphism from Palmprint Ridge Density among Malaysian Tamils for Person Identification.

Background: Fingerprints and palmprints form avaluable physical evidence in the scenes of crime.Palmprint ridge density is a vital tool used for personidentification during forensic investigation. Palmprintsare found impressed on the surface when a persontouches a surface. Aim and Objectives: This researchwas aimed to investgate the ridge density on six areas ina palmprint on both sides among Malaysian Tamils forperson identification. Material and Methods: Thisstudy sample consisted of 280 adult Malaysian Tamilsincluding140 males and 140 females. By inkingtechnique 560 palmprint samples were collected fromthe subjects for sexual dimorphism analysis. The sixprominent areas on the palmprints were chosen thatincluded Thenar region (P1), Hypothenar region (P2),Triradius of second digit (P3), Triradius of fifth digit(P4), Triradius of third digit (P5) and Triradius of fourthdigit (P6) for examination. Results: The resultsrevealed that the mean palmprint ridge density wassignificantly greater in females (10.26-12.03) thanmales (9.57-11.26) in all the designated areas (P1 toP6). The ridge density in the left hand was not themirror image of right hand, showing the bilateralasymmetry. Conclusion: The study findings revealedthat palmprint ridge density was sexually dimorphicamong Malaysian Tamil population.

Multiplex real-time PCR revealed very high prevalence of soil-transmitted helminth infections among aborigines in Peninsular Malaysia

Objective: To determine the true prevalence of soil-transmitted helminth infections in the Malaysian aborigines using real-time PCR. Methods: A total of 122 aborigines from seven tribes were recruited from settlements and nearby hospitals which served the communities, located in four states in Peninsular Malaysia. The stool samples were examined for the presence of soil-transmitted helminth using real-time PCR and microscopy. The latter included the direct wet mount and formalin-ether concentration technique (FECT). The infection load in FECT-positive samples was determined by the Kato-Katz method. Rotorgene real-time analyzer detected five helminth species using two sets of assays. Results: The real-time PCR detected soil-transmitted helminth in 98.4% samples (n=122), which were 1.56 times higher than by microscopy. Ascaris lumbricoides and Trichuris trichiura were detected in more than 90% of the samples, while hookworm was detected in 46.7% (Necator americanus) and 13.9% (Ancylostoma sp.) of the samples. Comparison with previous reports on the Malaysian aborigines showed that the real-time PCR markedly improved the detection of Ascaris lumbricoides, hookworm and Strongyloides stercoralis. The real-time PCR detected poly-helminths in 92.6% of the samples compared to 28.7% by microscopy. In addition, 27 samples (22.1%) showed amplification of Strongyloides stercoralis DNA. Conclusions: The real-time PCR showed very high prevalence rates of soil-transmitted helminth infections in the aborigines and is the recommended method for epidemiological investigation of soil-transmitted helminth infections in this population.