ABSTRACT
Objetivo. Evaluar la actividad antiproliferativa y genotóxica de una fracción con actividad citotóxica obtenida de la esponja marina del Caribe colombiano Topsentia ophiraphidites (Fracción T4). Materiales y métodos. La fracción T4 de la esponja marina Topsentia ophiraphidites fue obtenida en el laboratorio de Productos Naturales Marinos de la Universidad de Antioquia. La actividad antiproliferativa se evaluó mediante ensayos de eficiencia de clonación, función de acumulación y cinética proliferativa por intercambio de cromátidas hermanas (ICH); la actividad genotóxica se evaluó mediante electroforesis en gel de células individuales (Ensayo cometa) e intercambio de cromátidas hermanas (ICH). Todas las pruebas fueron realizadas sobre las líneas celulares Jurkat y CHO. Resultados. La fracción T4 afectó el ciclo celular de las células CHO y mostró daño genotóxica crónico en las células Jurkat. Conclusiones. Se recomienda la evaluación de la fracción T4 en otras líneas celulares derivadas de tumor con el fin de determinar un posible efecto diferencial, además de evaluar otras actividades de tipo antimicrobiano, antimalárico, entre otros.
Objective. To evaluate the antiproliferative and genotoxic activity of a fraction (T4 fraction) of the Colombian Caribbean marine sponge Topsentia ophiraphidites, with cytotoxic activity. Materials and methods. T4 fraction from the marine sponge Topsentia ophiraphidites was provided by the group of marine natural products from Universidad de Antioquia. The antiproliferative activity was evaluated by cloning efficiency tests, accumulation function, and proliferative kinetics by sister chromatid exchange (SCE), genotoxic activity was evaluated by SCE and gel electrophoresis of individual cells (Comet assay). All tests were performed on Jurkat and CHO cell lines. Results. The T4 fraction affected the cell cycle of CHO cells and presented chronic genotoxic damage in Jurkat cells. Conclusions. It is recommended to evaluate the T4 fraction in other derived tumor cell lines, in order to observe a possible differential effect, and to evaluate antimicrobial and antimalarial activities among others.
Subject(s)
Cytotoxicity, Immunologic , Genotoxicity , Marine FaunaABSTRACT
In the Camamu-Almada basin, marine fishery is exclusively small-scale, with several structural deficiencies such as boats with low or absent navigational technology, lack of credit and low income. Local fishers complain that shrimp and lobster trawling fishing is the main factor responsible for low stock abundance, but they still persist in these activities as these two species command the highest market prices. So they feel that the target species are already over-fished. We suggest that proper management action, alternative ways of income generation and the payment of job insurance would help to mitigate the problem.
Na bacia Camamu-Almada, as pescarias marinhas são exclusivamente de pequena-escala, com várias deficiências estruturais: com barcos quase sem nenhuma tecnologia de auxílio à navegação, falta de crédito e baixa lucratividade. Os pescadores locais reclamam que a pesca de arrasto dirigida a camarões e lagosta é a principal responsável pela baixa abundância dos estoques preferenciais, mas como eles ainda têm alto preço no mercado, essa estratégia de pesca ainda persiste. Assim, eles percebem que esses estoques estão sobre-pescados. Sugerimos que medidas apropriadas de manejo local, ocupações alternativas e o pagamento do seguro desemprego em dia para os pescadores ajudariam a mitigar essa situação.
Subject(s)
Adult , Animals , Humans , Fisheries/methods , Fishes/classification , Brazil , Fresh Water , Fisheries/economics , Interviews as Topic , Population Density , Seasons , Socioeconomic FactorsABSTRACT
Quantitative analyses of the pink shrimp (Farfantepenaeus brasiliensis and Farfantepenaeus paulensis) fisheries were carried out using data collected from July 1999 to July 2001 from the trawling operations of the fishing fleet based in Santos/Guarujá, SP. According to classical models, the fishery is at its maximum sustainable yield. Therefore, reduction of the fishing effort and adequate season and area closures seem to be the best management actions for the pink shrimp fishery.
Análises quantitativas do camarão-rosa (Farfantepenaeus brasiliensis e Farfantepenaeus paulensis) foram realizadas com dados coletados de Julho/1999 a Julho/2001, da frota de arrasto sediada em Santos/Guarujá (SP). De acordo com os modelos clássicos de manejo, a pescaria atingiu seu rendimento máximo sustentável. Assim, uma redução no esforço pesqueiro, combinada com a adoção de defesos periódicos e o fechamento de algumas áreas à pesca, parecem ser as melhores medidas de manejo do estoque para o camarão-rosa.