ABSTRACT
Introducción: Los pescadores en Playas del Coco, Guanacaste, Costa Rica, son vulnerables por el mal manejo y gestión de los recursos pesqueros y la pesca ilegal, excluyéndose de los beneficios otorgados por el Estado. Esto incide en que no cuenten con opciones que les permita crecer tanto a nivel económico como social. Objetivo: Determinar una estrategia de promoción social para las personas en exclusión social de Playas del Coco. Métodos: De febrero a septiembre 2018, se aplicó la Acción Participativa y la investigación Crítica, por medio de la entrevista semi estructurada y abierta, y la observación participante a miembros de la Asociación de Desarrollo, líderes comunales y pescadores de la zona, con edades entre los 17 y 40 años. Resultados: Se establecieron las bases teórico prácticas para un trabajo interdisciplinario entre trabajo social, turismo y biología marina por medio de la estrategia de intervención para los pescadores artesanales de Playas del Coco basada en el Modelo Socioeducativo Promocional y se documentó la situación actual de los habitantes de esta región. Conclusiones: La incursión del trabajo social en temas ambientales es viable, promocionando socialmente a los habitantes de Playas del Coco usando el turismo marino sostenible a través del modelo socioeducativo promocional.
Introduction: Fishermen in Playas del Coco, Guanacaste, Costa Rica, are vulnerable due to the mismanagement of fishing resources and illegal fishing; excluding them from benefits granted by the State. This means that they do not have many options that allow them to grow both economically and socially. Objective: Determine a social promotion strategy for people in social exclusion in Playas del Coco. Methods: From February to September 2018, participatory action and critical research were applied, through semi-structured and open interview, and participant observation to members of the Development Association, community leaders and fishermen in the area, ranging in age between 17 and 40 years old. Results: Theoretical and practical bases were established for an interdisciplinary work between social work, tourism and marine biology through the intervention strategy for fishermen of Playas del Coco based on the Socio-educational Promotional Model and the current situation of the inhabitants was documented. Conclusions: The incursion of social science in environmental issues is viable, by social promotion of the inhabitants of Playas del Coco developing sustainable marine tourism through a socio-educational model.
Subject(s)
Coasts , Tourism , Social Class , Costa Rica , Employment , Marine Biology/economicsABSTRACT
Background: Human immunodeficiency virus type 1 (HIV-1) infection and Acquired immunodeficiency syndrome are mayor global public health issues. HIV-1 infection is now manageable as a chronic disease thanks to the development of antiretroviral therapy; however, the existence of HIV drug resistance and collateral effects have increased the search for therapeutic alternatives. Compounds of marine resources have been studied for their antiviral potential. Objectives: To evaluate the antiviral activity of isolated bromotyrosine-derivative compounds from the Colombian marine sponges, Verongula rigida and Aiolochoria crassa against HIV-1 infection in vitro. Methods: Cytotoxicity of 11 bromotyrosine-derivative compounds was determined by the MTT assay. Inhibition of HIV-1 replication was performed using the U373-MAGI cell line, which was infected with recombinant green fluorescent protein (GFP)-expressing viruses pseudotyped, in the presence or absence of the compounds. The percentage of infected cells was evaluated by flow cytometry. In addition, the inhibition of reverse transcription and nuclear import was determined by quantification of early and late reverse transcription products and 2-LTR circles, respectively, using quantitative PCR. Results: Aeroplysinin-1, purealidin B and 3-bromo-5-hydroxy-Omethyltyrosine inhibited the HIV-1 replication in a dose-dependent manner, with a median maximum percentage of inhibition of 74% (20 µM), 57% (80 µM) and 47% (80 µM), respectively. Importantly, none of these concentrations were cytotoxic. Aeroplysinin-1, 19-deoxyfistularin 3, purealidin B, fistularin 3 and 3-bromo-5-hydroxy-O-methyltyrosine inhibited the nuclear import efficiently; while 3,5-dibromoN,N,N,O-tetramethyltyraminium, aeroplysinin-1, purealidin B, fistularin 3 and 3-bromo-5-hydroxy-Omethyltyrosine inhibited X4 HIV-1 cell entry with a median maximum percentage of inhibition ranging between 2 to 30%. Conclusions: Aeroplysinin-1, 19-deoxyfistularin 3, purealidin B, fistularin 3 and 3-bromo-5-hydroxy-O-methyltyrosine inhibited HIV replication at different steps. This study opens the possibility of chemically synthesizing these compounds and evaluating them as alternative therapies against HIV-1.