ABSTRACT
Objective To in depth analyze the chemical profile of Marsdenia tenacissima using HPLC-IT-TOF-MS. Methods The pulverized materials were exacted with methanol in an ultrasonication manner and then separated on a Waters Acquity UPLC HSS T3 column (100 mm × 2.1 mm, 1.8 μm, Milford, MA, USA) that was eluted in gradient with 0.1% formic acid-acetonitrile. The data was collected using automatically triggered tandem mass spectrometric mode in positive/negative ionization polarities. The mass fragmentation patterns of polyoxypregnane derivatives were proposed using some authentic compounds. Results Six chlorogenic acid derivatives and 15 polyoxypregnane derivatives were definitely assigned by referring to reference components, whereas the other signals, including 100 polyoxypregnane derivatives, four flavonoids, and two chlorogenic acid derivatives were tentatively annotated via matching the acquired information with those achieved information and the proposed mass fragmentation rules. Conclusion The research efficiently and accurately analyzed the chemical profile of M. tenacissima using HPLC-IT-TOF-MS, which will provide meaningful information for the quality evaluation and the therapeutic mechanism investigation of M. tenacissima as well as its preparation Xiao-Ai-Ping.
ABSTRACT
Objective: To optimize the testing methods for seed quality of Marsdenia tenacissima, and provide basis for establishing seed quality standard of M. tenacissima. Methods: The seed quality of M. tenacissima from different producing areas was measured based on the International Seed Testing Protocol made up by ISTA and Rules for Agricultural Seed Testing issued by China. Results: The samples weight of M. tenacissima were at least 900 g for purity analysis and were at least 90 g for testing. The 1 000-seed weight was determined by 500-seed method, and the water content was carried out by higher temperature (133 ± 2)℃ for 6 h. After soaking in water for 24 h, M. tenacissima seeds were cultured in wet sand at 30℃ for 1-8 d under illumination for germination testing. Seed viability was tested by TTC method in 35℃ for 3 h. Conclusion: The seed testing methods for quality items of M. tenacissima have been initially established.
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In order to identify the germination behavior of Marsdenia tenacissima seeds. We studied on ground substance, soaking time, temperature, illumination, and pH value, which have the effects on the germination of M. tenacissima seeds. The results showed that M. tenacissima seeds can germinate in different ground substances especially in sandy soil, the effect of soaking time on the seed germination of M. tenacissima is not significant, the seeds could germinate well during 25-30℃. Based on these appropriate conditions, the effect of illumination is not significant but the illumination should be the key factor under inappropriate temperature. The effect of acidic soil is lighter than that of alkaline soil. The optimal germination condition of M. tenacissima seeds is 24 h of soaking at the temperature of 30℃ with sandy soil by which pH value is 7.0-7.5 under illumination.
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Objective: To study the chemical constituents from the stems of Marsdenia tenacissima. Methods: The chemical constituents were isolated and purified by various column chromatographic methods. Their structures were identified on the basis of spectral data and chemical analysis. Results: Two pregnane glycosides were isolated from the stems of M. tenacissima and identified as 3-0-β-glucopyranosyl-(1→4)-6-deoxy-3-O-methyl-β-allopyranosyl- (1→4)-β-oleandropyranosyl-11α-O-tigloyltenacigenin B, named as tenacigenoside I (1); 3-O-β-glucopyranosyl-(1→4)-6-deoxy-3-O-methyl- β-allopyranosyl-(1→4)-β-oleandropyranosyl-11α, 12β-di-O-acetyltenacigenin B (tenacigenoside K, 2). Conclusion: Compound 1 is a new compound and compound 2 is obtained from the M. tenacissima for the first time.
ABSTRACT
Objective To study chemcial constituents in the canes of Marsdenia tenacissima.Methods Chemical constituents were isolated and purified by silica gel and ODS column chromatography.The structures were identified by means of physico-chemical and spectral data.Results From the 70% ethanol extract of the material,four compounds were isolated.Their structures were identified as 11?,12?-di-O-2-methylbutyryl-tenacigenin B (Ⅰ),11?-O-2-methylbutyryl-12?-O-acetyl-tenacigenin B (Ⅱ),tenacissoside H (Ⅲ),and marsdenoside A (Ⅳ),respectively.Conclusion Compound Ⅰ is a new C21 steroid compound,named tenacissoside O.