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Objective:To observe the effect of mechanical vibration on the expression of microRNA-214-3p and serum interleukin-1β (IL-1β) in the broken ends of fractured bones in ovariectomized rats.Methods:Thirty 3-month-old female Wistar rats were randomly divided into a control group, a model group, and a vibration group, each of 10. An operation of ovariotomy was performed in those of the model and vibration groups to establish osteoporosis model. Five months later a model of mid femur fracture was made with animals in all the groups. Five days after their fracture, the vibration group received 20 minutes of whole-body vibration treatment at 35Hz for 5 days a week, while the control group and the model group received natural rearing without any additional intervention At 2 and 6 weeks after the operation, the Lane-Sandhu X-ray scoring system was used to evaluate the quality of fracture healing, and reverse-transcription polymerase chain reactions and enzyme-linked immunosorbent assays were used to detect miR-214-3p in the fractured bones and the serum levels of IL-1β.Results:At 2 weeks and 6 weeks after the operation the average growth score of the broken ends in the model group was significantly lower than that of the control group, while that of the vibration group was significantly higher than the model group′s average. Compared with the control group at the same time point, the average miR-214-3p content of the model and vibration groups was significantly higher 2 and 6 weeks after the surgery. Compared with the model group, the average level of miR-214-3p of the fractured ends of the vibration group was significantly lower at 6 weeks. Two and six weeks after the surgery, the average IL-1β of the model group was significantly higher than the control group′s average, and that of the vibration group was significantly lower.Conclusion:Mechanical vibration can promote osteoporotic fracture healing by inhibiting the expression of miR-214-3p and reducing the level of IL-1β at the broken ends of fractured bones.
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BACKGROUND:Knee osteoarthritis is a common and frequently-occurring disease in the elderly, the incidence in the postmenopausal women is significantly higher than that in other groups. The clinical manifestation is various, mainly pain and dysfunction of the knee joint. There are many methods for the treatment of the knee osteoarthritis, and the non-operation treatment is the main and basic treatment method. OBJECTIVE:To summary the advantages and limitations of various non-operation treatment methods of knee osteoarthritis. METHODS:The VIP database, Wanfang database, CNKI database, Medline database and PubMed database were retrieved for the clinical reports and reviews on epidemiology, related factors, pathogenesis and non-operation treatment of knee osteoarthritis from January 2000 to May 2013 with the key words of“osteoarthritis, knee osteoarthritis, epidemiology, etiology, pathogenesis, treatment”in Chinese and “osteoarthritis, knee osteoarthritis, pathogenesis, therapy”in English. The reproductive researches and atypical reports were excluded. RESULTS AND CONCLUSlON:The etiology of knee osteoarthritis is unknown, and the pathogenesis may be related to various, of factors such as age, sex hormone, load conduction disturbance and genetic correlation. The clinical manifestations of knee osteoarthritis are varied, and many clinical treatment methods can be selected, but different treatment methods have different indications and the effects are not identical, and each has its own merits. Drugs are the most commonly used means for the treatment of knee osteoarthritis, such as traditional Chinese medicine, acupuncture and massage, which can significantly relieve the pain and improve the function of knee joint. Strength training and mechanical vibration are new methods of treatment, although not widely used in clinic, the effect has been gradual y confirmed, but stil need clinical exploration and experience accumulation. In addition, a large number of clinical data also showed that the oral drug in combination with life changing, exercise or muscle training therapy, its clinical effect is often better than single therapy.
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Objective To investigate the effects of different vibration durations on expression level of osteogenesis-related proteins by loading low intensity mechanical vibration in the ovariectomized (OVX) rats. Methods Twenty-four 6-month old female Wistar rats were ovariectomized and then randomly divided into 8-week-control group (C8), 12-week-control group (C12), 8-week-vibration group (V8), and 12-week-vibration group (V12). Vibration treatment was started one week after all the rats were ovariectomized. Rats in both V8 and V12 groups were loaded with 35 Hz, 0.25 g low intensity mechanical vibration for 15 minutes per day. C8 and C12 groups served as control without any treatment. Rats were executed in batch at 8th and 12th week, respectively, to analyze expression level of osteogenesis-related proteins, including BMP-2, p-ERK, Runx2 and OCN. Results Low-intensity mechanical vibration enhanced the osteogenesis related protein expression in OVX rats (P<0.01). With the extension of vibration duration, the osteogenesis related proteins BMP-2、p-ERK、Runx2 and OCN in V12 group were increased by 22.61% (P<0.05), 27.96% (P<0.01), 25.85% (P<0.01), 27.05% (P<0.01), respectively, as compared with V8 group. But for the control groups, no significant differences were found in expression level of osteogenesis-related proteins. Conclusions The low intensity mechanical vibration could elevate expression level of osteogenesis-related proteins, and the osteogenesis was enhanced with the extension of vibration duration.