ABSTRACT
Objective: To establish a study method for discovering quality marker of herb Meconopsis integrifolia (Non flower parts) based on spectrum-effect relationship for its quality control. Methods: UPLC fingerprints and UPLC-ESI-Q-TOF-MS/MS method were used to identify the chemical components of 10 batches of HMI extracts. The total antioxidant capacity, ABTS free radical scavenging ability, DPPH free radical scavenging ability and superoxide anion free radical suppression ability of 10 batches of sample were determined. Principal component analysis was used to screen antioxidant capacity index for HMI. Grey relational analysis was used to analyze the correlation between antioxidant capacity index and common peak of fingerprint spectrum. Then, quality marker could be preliminarily discovered by comprehensive analysis associated with antioxidant capacity index and efficacy-related constituents. Finally, the constituents were separated by HSCCC. Results: A total of 29 common peaks were identified from fingerprints of HMI extracts. 18 constituents were identified by UPLC-ESI-Q-TOF-MS/MS analysis, including 16 flavonoids and two alkaloids. DPPH scavenging ability was screened by principal component analysis which could reflect the antioxidant capacity of HMI extracts. Grey relational analysis showed that the correlations between the 29 common peaks and DPPH scavenging ability were all greater than 0.5. The quality markers of HMI were quercetin 3-O-β-D-glucopyrannosy-(1→6)-β-D-glucopyranoside and quercetin 3-O-[2'''-O-acetyl-β-D-galactopyranosyl-(1→6)-β-D-glucopyranoside] by comprehensive analysis. Among them, quercetin 3-O-[2'''-O-acetyl-β-D-galactopyranosyl-(1→6)-β-D- glucopyranoside] is a new compound. Conclusion: Study on the quality marker of HMI based on the spectrum-effect relationship is of great significance for elucidating the pharmacodynamic material basis, screening core quality marker related to medicinal effect, and ensuring the safety and rational application of traditional medicines.
ABSTRACT
Objective: To identify the common Tibetan traditional medicine of Meconopsis Vig. using nuclear gene internal transcribed spacer (ITS) and chloroplast psbA-trnH sequence. Methods: Ethnopharmacological study was carried out, three species of Ou-Bei (M. torquata, M. punicea, and M. integrifolia) and two species of Cai-Wen (M. racemosa and M. horridula) were collected. The ribosomal DNA ITS and chloroplast psbA-trnH noncoding region sequences were determined and analyzed. Results: The ITS sequences of M. racemosa and M. horridula were completely the same, while variable site could be detected in each pairwise comparison of ITS sequences in other species; The psbA-trnH sequence analysis showed that the variable sites could be detected in each pairwise comparison of the sequences. The combination of them could be used to identify all the five species. Conclusion: The combination of ITS and psbA-trnH sequence could be used to identify the Tibetan traditional medicine Ou-Bei and Cai-Wen in Meconopsis Vig.