Nephro-Protective Activity of Ethanolic Extract of Melia Azadirachta Against H2O2 Induced Toxicity in Vero Cell Line.

Renal disorders have become very common nowadays, which may also lead to kidney failure. The disorder may be caused by the commonly used chemicals such as acetaminophen, CCl4, streptromycin, H2O2 etc. The objective of this work is to determine the nephroprotective potential of ethanolic extract against H2O2 induced toxicity in VERO cell line. Ethanolic extract is known for its antioxidant, anti-inflammatory and anti-microbial effects, which make it a most sought for herbal medicine. Its characteristic features have identified this compound as a potential hepatoprotective and nephroprotective agent. VERO cells are cells taken from the kidney of an African green monkey, which are used in our study. The matured leaves of Melia Azadirachta were used to prepare ethanolic extract and the same was used to test for its inhibitory effect in 96 micro plate formats against in VERO cell lines. To study the cytotoxic properties of ethanolic extract against VERO cell line, we have tested the MTT assay with different concentrations in the range of 1000 to 62.5 μg/ml. From the performed assay, the effect of ethanolic extract drug reveals an enhanced activity on in VERO cell lines and that infers Melia Azadirachta, can be used as nephroprotective agent.

Suppressive Effects of Aqueous Extracts of Neem (Melia Azadirachta L.) on Some Initial Growth Parameters of Cow Pea & Horse Gram.

The suppressive effects of different concentrations of aqueous leaf extracts of Neem (Melia azadirachta L.) on cow pea (Vigna unguiculata L.) and horse gram (Dolichos biflorus L.).The experiment was conducted in sterilized petridishes with a photoperiod of 24 hours on an average temperature. The result showed that aqueous leaf extract of Melia azadirachta caused siginifican inhibitory effect on seed germination, root and shoot elongation and fresh & dry weight of receptor plants. The effect was proportional to the concentrations of the extracts and higher concentration had the stronger inhibitory effect. The study also revealed that inhibitory effect was much pronounced in shoot development than the root of cow pea. Likewise root development greatly inhibited than the shoot in horse gram.