ABSTRACT
Objective: To study the chemical constituents of Melilotus officinalis. Methods: The compounds were isolated and purified by silica gel column chromatography, gel chromatography, and ODS column chromatography. Their structures were elucidated by chemical and spectral analyses. Results: Twelve compounds were obtained from the 70% ethanol extract of M. officinalis and their structures were identified as β-sitosterol (1), coumarin (2), methyl 3-(4-hydroxyphenyl)propionate (3), kaempferol-3-O-α-L-rhamnoside (4), rutin (5), apigenin-7-O-β-D-lutinoside (6), dibutyl phthalate (7), robinin (8) clovin (9), kaempferol-3-O-rutinoside (10), isoquerecitrin (11), and medicarpin 3-O-β-glucopyranoside (12). Conclusion: Compounds 3, 4, 6, 7, 11, and 12 are isolated from plants from Melilotus Miller for the first time.