ABSTRACT
La trombosis venosa profunda del miembro superior es una entidad rara que se asocia con el uso de catéteres, estados de hipercoagulabilidad, anticonceptivos orales, neoplasias, síndrome de costilla cervical o de los escalenos, fracturas de clavícula y trombosis inducida por el esfuerzo. Se presenta un paciente de 53 años, del sexo masculino que desarrolló una trombosis de las venas axilar, cefálica y basílica tres días después de sufrir una fractura de tercio medio de clavícula que se inmovilizó inicialmente con vendaje en 8. El cuadro se resolvió con tratamiento con tinzaparina sódica y rehabilitación funcional; la fractura de clavícula consolidó y se recanalizaron las venas trombosadas. Se han reportado algunos casos de trombosis de las venas axilar o subclavia ocasionada por fracturas claviculares. Es muy infrecuente la aparición de trombosis extensa afectando a varias venas, en este caso, secundaria al traumatismo o a la presión ejercida por el vendaje(AU)
Upper-extremity deep vein thrombosis is a rare entity that is associated with the use of catheters, hypercoagulable states, oral contraceptives, neoplasias, cervical rib or scalene syndrome, clavicle fractures and stress induced thrombosis. A 53 year male patient is presented here. He developed thrombosis of the axillary, cephalic and basilica veins three days after suffering a fractured middle third clavicle, which was initially immobilized with eight-bandage. The condition was solved with enoxaparin sodium and functional rehabilitation; clavicle fracture consolidated and thrombosed veins had recanalization. Some cases of thrombosis of the axillary or subclavian veins caused by clavicular fractures have been reported. It is very rare the occurrence of extensive thromboses affecting several veins, in this case, secondary to trauma or pressure exerted by the bandage(AU)
La thrombose veineuse profonde du membre supérieur est une affection rare liée à l'usage de canules, à l'hypercoagulabilité, aux contraceptifs oraux, aux néoplasies, au syndrome de la côte cervicale ou du scalène antérieur, aux fractures de clavicule et à la thrombose induite par l'effort. Un patient âgé de 53 ans, ayant souffert une thrombose des veines axillaire, céphalique et basilique trois jours après une fracture du tiers moyen de clavicule et étant immobilisé au début par un bandage en forme de 8, est présenté. Le traitement a consisté à l'administration de tinzaparine sodique et la réhabilitation fonctionnelle ; la fracture de clavicule a consolidé et les veines affectées par la thrombose ont été récanalisées. Quelques cas de thrombose de la veine axillaire ou sous-clavière provoquée par des fractures claviculaires ont été rapportés. La survenue d'une thrombose étendue affectant plusieurs veines, dans ce cas après un traumatisme ou une pression exercée par un bandage, est très peu fréquente(AU)
Subject(s)
Humans , Male , Middle Aged , Clavicle/injuries , Upper Extremity Deep Vein Thrombosis/complicationsABSTRACT
Objective To study the tumorigenetic characteristics of side population (SP) cells and their exsistence in gastric cancer cell lines and tissues.Methods Flow cytometry and the DNA-binding dye Hoechst 33342 staining were used to analyze sorted SP cells in 3 gastric cancer cell lines(SGC-7901,BCIC-823 and MKN28).SP cells and non-SP cells(main population cells)isolated from SGC-7901 cell line weresubcutaneously injected into 36 nude mice with 500,5000 or 50 000 cells per mouse,respectively.Thetumorigenity was observed 8 weeks after injection.The expression of ATP-binding cassette sub-family Gmember 2(ABcG2)mRNA in three gastric cell lines and its level in gastric cancer tissues were detected by real-time PCR and immunostaining.respectively.Results The proportion of SP cells was accounted for 1.0%in SGC-7901 cells and 1.3%in BGc-823 cells.No SP cell was found in MKN28 cells.As low as 500 SP cells or 50 000 non-SP cells could initiate tumors in mice.The expression of ABcG2 mRNA was higher in SGC-7901(O.162)and BGC-823(O.096)cell lines than that in MKN28 cell line(0.005).The value of AtCG2 mRNA/beta-aetin mRNA and protein in gastric cancer tissues was different from those in gastritis tissues.Condasimm SP cells have strong ability of tumorigenesis compared with non-SP cells.The expression of ABCG2 is found in gastric cancer tissues and part gastritis tissues.The more the SP cells in gastric cell lines,the higher the expression of ABCG2.