Metabolic study of Mori Cortex on nephrogenic edema in rats / 中成药

AIM To conduct a metabolic research for a better understanding of nephrogenic edema and to assess the integral efficacy of Mori Cortex in rat model.METHODS The serum creatinine,urea nitrogen,albumin and urinary protein levels in rats were detected.UPLC-QTOF-MS was used to detect the urine metabolites changes,Principal Component Analysis (PCA) and Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA)were used to screen potential biomarkers,after whose quantification,Mev software was adopted for heat map draw-ing and hierarchical cluster analysis.RESULTS The model rats manifested significantly increased levels of serum creatinine,urea nitrogen and urinary protein,decreased albumin level,and an obviously excessive amino acid metabolism as well.The 41 identified biomarkers were mainly related to disturbances in phenylalanine,pyrimidine,arginine and proline,glycine,serine and threonine,tryptophan,cysteine and methionine metabolism,and biosynthesis of pantothenic acid and coenzyme A.A reversal trend in aforementioned levels of biochemical indexes and most biomarkers due to the intervention by Mori Cortex signaled an improvement in the metabolic disorder,renal dysfunction and edema.CONCLUSION The metabolic study demonstrates the pathological status of nephrogenic edema and assesses the effect of Mori Cortex from an overall perspective,highlighting a new approach for illustrating Chinese medical syndrome and the underlying mechanism in the management of traditional Chinese medicine.

Estudio metabólico en pacientes con urolitiasis de alto riesgo de recurrencia / Metabolic study in patients with urinary lithiasis at high risk of recurrence

Introducción: La urolitiasis es considerada actualmente una enfermedad metabólica con tendencia ala recurrencia. El objetivo de este trabajo es evaluar la prevalencia de alteraciones metabólicas en pacientes de alto riesgo y su impacto según sexo y edad. Materiales y métodos: Es un estudio descriptivo de 36 pacientes (25 hombres y 11 mujeres), portadores de patología litiásica con alto riesgo de recurrencia. El estudio metabólico consistió en: calcemia, uricemia, fosfemia, PTH sérica, calciuria/24 hrs, uricosuria/24 hrs, fosfaturia/24 hrs, oxalaturia/24 hrs,citraturia/24 hrs y creatininuria/24 hrs. Los valores obtenidos fueron ajustados de acuerdo a la creatininuria y peso. Para el análisis estadístico se utilizó t-student (STATA 7.0). Se consideró significativo p<0,05.Resultados: En el 69 por ciento (25/36) se observó alguna alteración metabólica; el 36 por ciento (13/36) presentó 2 omás alteraciones metabólicas. Las alteraciones más frecuentes fueron la hipercalciuria (30,6 por ciento; 11/36), la hipocitraturia (30,6 por ciento; 11/36), la hiperuricemia (19,4 por ciento; 7/36) y la hiperoxalaturia (13, por ciento; 5/36).No se observó diferencias significativas de edad o sexo entre los grupos con y sin alteración metabólica. Conclusiones: La mayoría de los pacientes con patología litiásica recurrente o de alto riesgo presentan una o más alteraciones metabólicas, predominando la hipercalciuria y la hipocitraturia. En este estudio no hubo diferencias entre ambos sexos en la mayoría de las alteraciones metabólicas, ni tampoco en su distribución etaria. Estos resultados demuestran la necesidad de realizar estudios metabólicos en pacientes de alto riesgo, dado que existen herramientas terapéuticas que permiten un manejo médico de las alteraciones metabólicas y de esta forma reducir la recurrencia de litiasis.

Introduction: Urolithiasis is a metabolic disorder with a tendency to relapse. The aim of this study was to assess the prevalence of metabolic abnormalities in patients at high risk and the impact of sex and age. Materials and methods: Descriptive study of 36 patients (25 men and 11 women),with lithiasic pathology at high risk of recurrence. The metabolic study included the measurement of calcemia, uricemia, fosfemia, parathormone, calciuria/24hrs, uricosuria/24hrs, fosfaturia/24hrs, oxalaturia/24hrs, citraturia/ 24hrs and creatinine/24hrs. The values obtained were corrected according to weight and creatinine. The test used for statistical analysis was t-student (STATA 7.0). It was considered significant p <0.05.Results: In 69 percent (25/36) of the cases a metabolic abnormality was observed and in 36 percent (13/36) there was 2 or more alterations present. The metabolic disorders most frequently observed were hypercalciuria (30.6 percent; 11/36), hypocitraturia (30.6 percent; 11/36), hyperuricemia (19.4 percent; 7/36) and hyperoxaluria (13.9 percent; 5/36). There was no significant difference in age or sex between the groups with and without metabolic abnormality. Conclusions: Most patients with recurrent lithiasic pathology or at high-risk display one or more metabolic disorders, being hypercalciuria and hypocitraturia the most frecuently encountered. In this study, there was no difference between sexes in most of the metabolic disorders, nor in its age distribution. These results demonstrate the need for metabolic studies in high-risk patients, since there are tools that allow therapeutic medical management of metabolic disorders and thus reduce the recurrence of lithiasis.

Comparison of Stone Metabolic Risk Factors in Recurrent Stone Formers according to Sex and Age / 대한비뇨기과학회지

PURPOSE: In order to compare stone metabolic risk factors in recurrent stone formers, we researched lithogenic factors according to sex and age. MATERIALS AND METHODS: We performed stone metabolic studies on recurrent stone formers (77 men, 33 women) and grouped them according to age; under 40 (n=38), from 40 to 59 (n=56) and over 60 (n=16). We evaluated excretion rate differences of lithogenic and inhibitory constituents such as volume, sodium, uric acid, calcium, oxalate, and citrate from 24-hour urine samples, and calcium, sodium, uric acid, phosphate, potassium, and chloride from serum samples. We analyzed the incidence of stone metabolic risk factors between the age groups and the sexes. RESULTS: The most common detectable risk factor in all groups of recurrent stone formers was hypocitraturia. This finding was more remarkable in males aged under 60. The incidence of hypocitraturia was higher in males and in the younger group, whereas hypercalciuria was more frequent in the elder group. CONCLUSIONS: The most important cause of urolithiasis was hypocitraturia in recurrent stone formers, and this was apparent in males aged under 60.

Biochemical evaluation of 24-hour urine in stone patients / 대한비뇨기과학회지

The fifty four patients with urinary stones(38 men. 16 women) and nine controls on usual constant diet were evaluated with the measurement of urinary minerals. electrolytes, citrate and calculation or net gastrointestinal absorption or alkali by recently devised simple method, i.e., (Na+K+ Ca + Mg)-(CI +1.8P) of urine, to evaluate prevalence of either hypo-or hyper-excretion of each items as well as to see possible correlation between citraturia and net gastrointestinal absorption of alkali. In 24-hour urine measurement, the stone patients in comparison with controls showed hyperexcretion of calcium(p<0.05), oxalate(p<0.05) and sodium(p<0.05) and hypoexcretion of phosphorus( p<0.05), potassium(p<0.001) and citrate(p <0.05). Hypocilraturia(less than 320mg/ dl) was noted in 64.8% of all stone patients though mean urine citrate levels were higher in women compared to men without statistical significance. In view of gender difference, all 24-hour urine analysis except citrate in stone patients were higher in men than women. of which calcium, creatinine, potassium and chloride were statistically significant(p<0.05). A retrograde analysis between citraturia and net gastrointestinal absorption of alkali in both stone patients and controls didn`t reveal any significant correlation. In conclusion, 24-hour urine biochemistries are an influential factor or the stone formation and this study regarding to relation between hypocitraturia and reduced net gastrointestinal absorption of alkali shows no correlation."

Bio-chemical assessment of stone metabolic study in patients with calcium oxalate urolithiasis / 대한비뇨기과학회지

A study was done on 200 patients with a diagnosis of calcium oxalate stone and 50 cases of control group to evaluate the chemical relationships between stone formation and a 24-hour excretion or calcium. oxalate, uric acid and citrate. This study was also evaluated by comparing urinary concentrations and total daily output of the above metabolites. Among the 200 patients metabolic disorders included hypercalciuria in 34 (17.0%), hyperoxaluria in 8 (4.0%), hyperuricosuria in 43 (21.5%) and hypocitraturia in 128 (64.0%). The total output of calcium, oxalate, uric acid, citrate were significantly different (p<0.01) and also showed significant differences in the those concentrations between these two groups. Therefore, it is confirmed that the concentration of stone metabolite is also a influential factor of the stone formation as like as total daily output.