ABSTRACT
The chronic toxic effect of Metanil Yellow on the gills of freshwater teleost Heteropneustes fossilis (Bloch) was studied. The ?shes were exposed for 45 days treatment in 2g/L of Metanil Yellow. However, after 45 days treatment, marked pathological changes in the gills were found. Necrosis found in the primary gill lamellae. Shortened and clubbing ends of the secondary gill lamellae. Buldging tip of primary gill ?laments and distortion was occurred in the shape of secondary ?lament. Pycnotic nuclei, vacuolization and degenerative epithelial cells were found
ABSTRACT
Two simple, accurate, rapid and sensitive Methods (A and B) have been developed for the estimation of Alosetron in its pharmaceutical dosage form. The Method A is based on the formation yellow colored chromogen, due to reaction of Alosetron Hydrochloride with Metanil yellow dye, formation of ion association complexes of the drug with dyes in phosphate buffer of pH 3.6 followed by their extraction in chloroform which exhibits λmax at 410 nm. The Method B is based on the formation of light yellow colored chromogen due to reaction of Alosetron Hydrochloride with Methyl Orange dye, formation of ion association complexes of the drug with dyes in phosphate buffer of pH 3.6 followed by their extraction in chloroform, which exhibits λ max at 422 nm. The absorbance-concentration plot is linear over the range of 5-60 mcg/mL for Method A and 50-120 mcg/mL for Method B. Results of analysis for all the methods were validated statistically and by recovery studies. The proposed methods are precise, accurate, economical and sensitive for the estimation of Alosetron in bulk drug and in its Tablet dosage form.