Cuantificacion de arsenico por inyeccion en flujo-generacion de hidruros-espectrometria de absorcion atomica (IF-GH-EAA) previa derivatizacion con L-cisteina. Validacion y comparaci¨®o intermetodologica utilizando dos tecnicas de referencia / Arsenic quantitation by flow injection-hydride generation- atomic absorption spectrometry (FI-HG-AAS) after L-cysteine derivatization. Validation and inter-methodological comparison using two reference techniques

La presencia de Arsenico (As) en aguas de consumo representa una problematica para la salud publica en muchas regiones del mundo, incluida la Argentina. La cuantificacion de arsenico en agua de bebida y en orina se utiliza para evaluar la exposicion a este contaminante. El presente trabajo tuvo como objetivo la validacion metodologica de una tecnica para la cuantificacion de especies del As [AsV + AsIII + acido monometilarsonico (MMA) + acido dimetilarsinico (DMA)] por inyeccion en flujo-generacion de hidruros-espectrometria de absorcion atomica (IF-GH-EAA), previa derivatizacion con L-cisteina. Los resultados fueron comparados con los obtenidos utilizando dos metodologias de referencia, generacion de hidruros-espectrometria de absorcion atomica (GH-EAA) para muestras de aguas y orina, y cromatografia de alta resolucion ¨Cgeneracion de hidruros-espectrometr¨ªa de absorcion atomica (HPLC-GH-EAA) para muestras de orina. Ademas, se evaluo la selectividad de la cuantificacion por IF-GH-EAA, en presencia de otras especies quimicas del As, provenientes del consumo de alimentos producto de la pesca, a traves de un ensayo biologico. Los niveles de As hallados en las muestras de agua y de orina utilizando las tecnicas de referencia presentaron un rango de 6 a 176 ¦Ìg/L y de 143 a 3312 ¦Ìg/g de creatinina, respectivamente. Los coeficientes de Pearson resultantes de la comparacion de los datos obtenidos por IF-GH-EAA, con los logrados por los metodos de referencia fueron r = 0,9976 y r = 0,9422, para agua y orina, respectivamente. Los resultados de la prueba biologica indicaron un mayor nivel de As, debido al consumo de alimentos producto de la pesca, cuando las muestras de orina fueron previamente mineralizadas (GH-EAA), con la consecuente sobreestimacion del contenido de As proveniente del consumo de As inorganico. Este aumento no se observo cuando estas fueron analizadas por IF-GH-EAA ...

The presence of arsenic (As) in drinking water is a public health concern in many regions of the world, including Argentina. Quantification of arsenic in drinking water and urine are used to assess exposure to this pollutant. This study aimed to validate a methodology for the quantification of As species [AsV + AsIII + acid monometilarsonico (MMA) + dimetilarsinico acid (DMA)] by flow injection-hydride generation-atomic absorption spectrometry (FI-HG-AAS), after derivatization with L-cysteine. The results were compared with those obtained using two methods of reference, hydride generation-atomic absorption spectrometry (HG-AAS) for water and urine samples, and high performance liquid chromatography-hydride generation-atomic absorption spectrometry (HPLC-HG-AAS) for urine samples. In addition, the selectivity of quantification by FI-HG-AAS in the presence of other chemical species of As, from fishery products intake, was evaluated through a biological assay.The As level found in water and urine samples, using the techniques of reference, showed a range from 6 to 176 ¦Ìg/L and from 143 to 3312 ¦Ìg/g creatinine, respectively. Pearson coefficients resulting from the comparison of data obtained by FI-HG-AAS with those achieved by the reference methods were r = 0.9976 and r = 0.9422 for water and urine, respectively. The results of the biological test showed a higher level of As, due to consumption of food fishery product, when urine samples were previously mineralized (HG-AAS), with consequent overestimation of the inorganic arsenic consumption. When these samples were analyzed by FI-HG-AAS this fact was not observed, and the values were comparable to baseline level prior to consumption ...

Validation on Method of Microbial Limit Test of Povidone Iodine Solution / 中国药房

OBJECTIVE:To establish a microbial limit test method for povidone iodine solution.METHODS:Validation on methodology of the microbial limit test of povidone iodine solution was performed using plating method(Ⅰ),culture agent dilution method(Ⅱ),membrane-filter procedure method(Ⅲ)and sodium thiosulfate neutralization in combination with membrane-filter procedure method(Ⅳ).RESULTS:The recovery rates of all of the test organisms in method Ⅰ and method Ⅱ approached zero,less than 70% in method Ⅲ but above 85% in method Ⅳ.The growth of the control bacteria was abnormal in method Ⅱand method Ⅲ,but normal in method Ⅳ.CONCLUSION:It is advisable to adopt the method Ⅳ for microbial limit test of povidone iodine solution.

Methodological Validation on Microbil Limit Test of Dental Ulcer Pellicles of Sunset Abelmoschus Flower / 中国药房

OBJECTIVE:To validate the test condition and test method of the microbil limit test of the Dental ulcer pellicles of Sunset Abelmoschus Flower.METHODS:The methodological validation was carried out in accordance with the standard for microbil limit test stated in the appendix of China Pharmacopoeia(2005 Edition,VolumeⅠ).RESULTS:By membrane-filter procedure,the recovery rates of different test strains in the samples were all over 70%.CONCLUSION:The membrane-filter procedure can more effectively eliminate the bacteriostatic action of Dental ulcer pellicles of Sunset Abelmoschus Flower so as to result in accurate microbil limit test results.