ABSTRACT
【Objective】 To investigate the cell death-inducing effect of methyl rosmarinate (MR) on human hepatoma Hep-3B and SK-Hep1 cells and their potential mechanisms. 【Methods】 The effects of MR on the viability of Hep-3B, SK-Hep1 and MIHA cells were determined by cell counting kit-8 (CCK-8) assay. The morphological changes of three kinds of cells treated with different concentrations of MR were observed by optical microscopy. EdU assay and flow cytometry were used to detect the proliferation and apoptosis of Hep-3B and SK-Hep1 cells. Transwell assay was used to study the effects of MR on the migration and invasion of Hep-3B and SK-Hep1 cells. Western blotting was used to evaluate the protein expression levels of apoptosis, EMT and Akt/mTOR signaling pathways. 【Results】 After treated with different concentrations of MR (0~200 μmol/L) for 48 h, Hep-3B and SK-Hep1 cells activities were significantly decreased in a concentration-dependent manner (P<0.01), while there was no significant effect on MIHA cell activity (P>0.05), and the IC50 of Hep-3B and SK-Hep1 cells were 102.5 and 99.3 μmol/L, respectively. MR treatment (0-150 μmol/L) significantly inhibited the proliferation of Hep-3B and SK-Hep1 cells (P<0.05), while cell detachment and shrinkage were observed by optical microscopy on the Hep-3B and SK-Hep1 cells, while the morphology of MIHA cells was not changed. Compared with the control group, MR (100, 150 μmol/L) induced apoptosis in Hep-3B and SK-Hep1 cells, the expression levels of the pro-apoptotic proteins Bax and cleaved PARP were significantly increased (P<0.05), while the expression level of the anti-apoptotic protein Bcl-2 was significantly decreased (P<0.05). MR (100, 150 μmol/L) also inhibited the migration and invasion of HCC cells, significantly increased the expression of E-cadherin and decreased the expression of N-cadherin and Vimentin compared with the control group (P<0.05). Finally, Western blotting results showed that the expressions of p-Akt and p-mTOR in Hep-3B and SK-Hep1 treated by MR were significantly reduced in a dose-dependent manner, suggesting that MR may play an anti-cancer role by inhibiting Akt/mTOR signaling pathway. 【Conclusion】 MR can promote apoptosis in Hep-3B and SK-Hep1 cells, which may be closely related to the inhibition of Akt/mTOR signaling pathway.
ABSTRACT
OBJECTIVE: To study the polar chemical constituents from Prunella vulgaris L. METHODS: Silica gel, reverse-phase octadecylsilyl(ODS), Sephadex LH-20 chromatographic methods, MCI and HPLC were applied to isolate and purify compounds. MS and NMR methods were used to determine the structures of the compounds. Furthermore, the cytotoxicity of these chemical components for MCF-7, MDA-MB-231 and MCF-10A cell lines was measured by MTT method. RESULTS: A total of 12 compounds were isolated from the fruits of P. vulgaris and their structures were identified as methyl 3,4,α-trihydroxypropionate(1), danshensu(2), methyl rosmarinate(3),3,4-dihydroxybenzoic acid(4), quercetin-3-O-glycopyranoside(5), hyperoside(6), 2α,3α,19α,24-tetrahydroxylurs-12-en-28-oic acid(7), 2α, 3α, 24-trihydroxyolean-12-en-28-oic acid(8), cytidine(9), daucosterol(10), (3S,5R,10S)-7-oxo-12-methoxyabieta-8,11,13-triene-3,11,14-triol(11), and 2α,3α,24-trihydroxyolean-12,20(30)-dien-28-oic acid(12). The results of antitumor assay indicated that compound 2,3,5 and 6 significantly inhibited the activity of MCF-7, compound 3 could inhibit the activity of MDA-MB-231, but all of them also significantly inhibited the activity of normal cell lines MCF-10A. CONCLUSION: Compounds 9 and 11 are isolated from the genus of Prunella L. for the first time. Some chemical constituents form Prunella L. show certain anti-breast cancer activity.
ABSTRACT
Objective: To investigate the chemical constituents from the aerial part of Rabdosia flexicaulis. Methods: Various column chromatography techniques were used to isolate and purify the compounds and their structures were identified by the spectral data. Results: Fifteen compounds were isolated and identified as rotundic acid (1), tormentic acid (2), corsolic acid (3), 23-hydroxyursolic acid (4), maslinic acid (5), teuclatriol (6), 3,6-dihydroxy-1-menthen (7), 1- hydroxypinoresinol (8), epipinoresinol (9), lariciresinol (10), caffeic acid (11), rosmarinic acid (12), 3'-O-methyl-rosmarinic acid (13), methyl 4'-O-methyl-rosmarinate (14), and methyl 3-dehydroxyl-rosmarinate (15). Conclusion: All the compounds are isolated from the species for the first time, among which compounds 1, 6, 7, 9, 13, and 14 are isolated from the plants in genus Rabdosia (Bl.) Hassk. for the first time.
ABSTRACT
Objective: To investigate the chemical constituents from Vitex negundo. Methods: The chemical constituents were separated and purified by column chromatography including ODS, Sephadex LH-20, and silica gel columns. Their structures were elucidated on the basis of physicochemical properties and spectral data. Results: Eleven compounds were identified as isoorientin (1), vitegnoside (2), luteolin-7-O-β-D-glucopyranoside (3), isovitexin (4), luetolin-3'-O-β-D-glucuronide (5), apigenin-7-O-β-D-glucoside (6), kaempferol-3-O-β-D-glucopyranoside (7), methyl rosmarinate (8), 5-O-caffeoyl quinic acid methylester (9), caffeic acid (10), and grevilloside G (11), respectively. Conclusion: Compounds 3 and 4 are isolated from V. negundo for the first time, and compounds 5-11 are firstly obtained from the plants of Vitex Linn.