ABSTRACT
【Objective】 To understand the frequency and significance of anti-" Mia" (anti-" Mia" mixtures of antibodies) in local population in Zhongshan, and the influence of different experimental conditions on the activity of human anti-" Mia" . 【Methods】 The microplate-based agglutination assay and polybrene method were used to screen anti-" Mia" in 3 587 blood samples from voluntary blood donors and patients using O type red blood cells with positive Mia antigen, then.rechecked by tube method and microcolumn gel card method. 【Results】 The frequency of anti-" Mia" was 1.06% (38/3 587), among which 60.5% (23/38) were IgM and 39.5% (15/38) were mixture of IgM and IgG; 0.61% (13/2 135) in local blood donors and 1.72% (25/1 452) in patients(P<0.01). 65.8% (25/38) of the population carrying anti-" Mia" had a history of immunity. 57.9% (22/38) were identified to be anti-" Mur" and 42.1% (16/38) anti-" Mia" using GP.Vw erythrocyte. The appropriate incubation time for anti-" Mia" test was 10 min. 【Conclusion】 The frequency of anti-" Mia" was relatively high among blood donors and patients in Zhongshan, and most of the anti-" Mia" carriers had a history of immunity. Most anti-Mia antibodies were active in saline, and some of them were mixture of IgM and IgG. It may be helpful to include Mia positive red blood cells in the irregular antibody screening cell panel to improve the safety of blood transfusion.
ABSTRACT
The Miltenberger (Mi) classes represent a group of phenotypes for red cells that carry low frequency antigens associated with the MNSs blood group system. This pilot study was aimed at determining the Mia antigen positivity in the blood donor population in a tertiary care hospital in New Delhi, India. The study was performed between June to August 2014 on eligible blood donors willing to participate. Antigen typing was performed using monoclonal anti-Mia antiserum by tube technique. Only one of the 1000 blood donors (0.1%) tested was found to be Mia antigen positive. The Mia antigen can, therefore, be considered as being rare in the Indian blood donor population.