The Frequency of Unexpected Antibodies in Blood Donors and Transfusion Candidates in Korea / 대한수혈학회지

BACKGROUND: A retrospective study was performed to estimate the frequency of red cell antibodies in blood donors (n=1,620,023) and transfusion candidates (SNUH n=12,111, YUMH n=26,665) for last 2 years (2000~2001). The results of the antibody screening and identification tests, the frequency and specificities of antibodies identified compared with blood centers and two hospitals had been used the different test methods each others. METHOD: Blood centers had been used tube and micro-plate method simultaneously with an in house and commercial panels. SNUH had been used micro-plate method using V plate with an in house and commercial panels. YUMH had been used gel agglutination technique (DiaMed ID System : DiaMed, Murten; Switzerland) since 1998. RESULTS: The frequencies of red blood cell antibodies were 0.26% (4,204 / 1,620,023 donor sera ), 0.11% (135 / 12,111 patient sera in SNUH) and 0.48% (128 / 26,665 patient sera in YUMH). Female donors and old ages showed higher frequency of red cell antibodies than male and young ages. Most of antibodies detected in donors were clinically less relevant antibodies such as Anti-Lea and Leb (38.9%), anti-P1 (18.1%), anti-H(IH) (8.4%), anti-M (6.2%) and so on. Clinically significant antibodies including Rh system antibodies (2.0%) were few, and composed only 12% of all the antibodies detected. In patients, clinically relevant antibodies including Rh antibodies (40.4% in SNUH, 71.9% in YUMH) were more frequently observed comparing with in donors. CONCLUSION: Antibodies found in donors were mostly clinically less relevant. Antibody screening method used in blood centers would be standardized. Blood banks using gel technique showed high detection rate of clinically significant antibodies comparing with facilities using other methods.

Establishing a trapezoid micro-plate method to detect ABO blood group in serum / 中国输血杂志

Objective Establishing a trapezoid micro-plate method to detect ABO blood group in serum.Methods Select 32 blood samples containing A,B,O or AB types randomly.Use orthogonal design principle to select the optimum conditions of the blood plasma dilution,erythrocytes density,temperature and static time.The mix-blood samples are serially diluted.The agglutinating and nonagglutinating gray zones are also defined.Based on the optimum conditions and criterions,the reproducibility test,sensitiveness test,accuracy test,lipaemia interference test and haemolysis interference test are carried out.6 ABO subgroup(AM,AX,A2B,A3B,AXB,BM) and 5 irregular antibody(anti-M,anti-N,anti-P1,anti-Leb,anti-HI) are detected to evaluate the ability of the trapezoid micro-plate method to detect special samples.Results The trapezoid micro-plate method's repetitiveness tests coincidence is 100%.Its sensitivity is identical to the test tube method.The accuracy is 99.99%.In the 47 various kinds of lipaemia blood samples' anti-interference tests,the correct interpretation at one time is 100%.In the haemolysis interference tests,when hemoglobin content are below 20g/L,there are no obvious influences found.When sever haemolysis appears(blood plasma hemoglobin content are above 20g/L),there is no effect on the agglutination,but make a difference to the nonagglutinating.The ability to dectect 6 ABO blood subgroups and 5 irregular antibody blood samples is great.Conclusion The trapezoid micro-plate method not only has good ability to detect the ABO blood group both in the red cell typing and serum typing,but also enhances the accuracy in detecting the agglutinating blood samples and improves the exactitude of the tests.