Cultura, subjetividade e as organizações na contemporaneidade / Culture, subjectivity and the organizations in contemporaneity

Inserido numa perspectiva histórico/político/social, pretendemos, neste artigo, abordar operadores conceituais, tais como: cultura, subjetividade e microcultura organizacional relacioná-los à dinâmica e aos atuais modos de funcionamento das organizações. Articulação que os reconhece como campos de força que produzem o "jeito de ser" do trabalhador no contexto da ambiência das empresas. Adotaremos como ponto de partida o conceito de cultura por considerá-lo central para a tarefa proposta, apresentando, na sequência, um breve panorama dos determinantes políticos e sociais presentes na era industrial. Seguindo uma lógica que elege como fio condutor as repercussões da conjunção do político e do social sobre o modo de ser dos trabalhadores, apresentaremos a subjetividade como produto do social e, nesta perspectiva, percebemos mutações significativas, sobretudo a partir da emergente globalização da economia e do advento da sociedade informacional. Para possibilitar uma melhor compreensão da dinâmica organizacional, abordaremos algumas particularidades de sua microcultura, na medida em que engendram novos modos de ser do trabalhador. Pretendemos, ao final, refletir sobre as possibilidades de construção de novos agenciamentos de singularização que se apresentam como fontes de emancipação para o trabalhador frente aos desafios que se revelam nas configurações e nas tendências do mundo corporativo. Esperamos contribuir para uma maior compreensão acerca da saúde do trabalhador, considerada também como resultado de sua inserção no mundo e do sentido que ele dá às atividades que executa.

Inserted in a historical/political/social, one aims, in this article, at dealing with conceptual operators, such as culture, subjectivity and organizational microculture and aims at relating them to organization operation dynamics and current ways. Articulation that recognizes them as strength fields that produce the worker´s "way of being" in the enterprises ambience context. We will assume, as a starting point, the concept of culture since we consider it truly central - as a kind of nucleus - for our intended task, showing out sequentially the political and social determinants brief panorama, present in our industrial era. Following up a logical that elects, as a leading path, the political and social instances conjunction repercustions regarding the workers´ way of being, it shows up subjectivity as the social environment and subjects product and, in this perspective, we may realize, perceive meaningful mutations, overall, departing from economy emergent globalization and the informational society advent, i. e. arrival. In order to make a better comprehension about the organizational dynamics, some of its microculture particularities will be approached, according as they engender the worker´s new ways of being. We aim, at last, at reflecting about the possibilities of construction of singularization begetters that are viewed as emancipation sources for workers in front of challenges that are revealed in the corporative world configurations and tendencies. We hope having contributed for a greater understanding about workers´ health, viewed also as a result of this insertion into the world and the meaning he confers to the activities he develops.

A new culture system for in situ observation of the growth and development of Eucyclops serrulatus (Copepoda: Cyclopoida)

A practical and convenient method of rearing Eucyclops serrulatus in a microculture environment is described. A complete life cycle of E. serrulatus was maintained in a narrow space on a microscope slide glass on which a cover glass of 22 x 40 mm in size was mounted at a height of 0.8 mm. The culture medium was constituted by bottled mineral water boiled with grains of Glycine max (soybean). Chilomonas paramecium, a free-living protozoan organism, was provided as live food. Growth of nauplii hatched from eggs to the first stage of copepodite took an average of 7.7 days, and the growth of copepodite 1 to the egg-bearing adult female took an average of 20.1 days in the microculture cell with an average life time of 44.7 days. Continuous passage of copepods was successfully maintained as long as sufficient medium and food were provided. The microculture method enables an in situ microscopic observation on the growth and developmental process of helminth larvae experimentally infected to copepods as well as of copepod itself. Furthermore, it does not require anesthetization and, therefore, minimize the amount of stress exposed to copepods during the handling process.

Antimycotic susceptibility testing of trichophyton rubrum by microculture method / 영남의대학술지

Various susceptibility tests have been used to determine minimal inhibition concentration (MIC) of dermatophytes. They have limitations to apply practically because they need long time to determine MiC. Authors examined MIC of T. rubrum to ketoconazole and itraconazole using 96- well microplate and 24-well macroplate by method of Granade and Artis and tried to check the possibility of this method on clinical application. Nine strains of T. rubrum from patients with dermatophytosis were used. Evaluations of the factors affecting MIC were also tried. The results as follows. 1. Effect of inoculation density on determination time and MIC: Determination of MIC were possible in 4th days after inoculation at higher inoculation density (aborbance 2.0, 1.0) compared to 6th days at lower inoculation density (absorbance 0.5, 0.25). 2. Effect of incubation temperature on MIC: When incubating at 37℃, MIC were below 0.006-0.04µg/ml to ketoconazole and below 0.006-0.04µg/ml to itraconazole while at 25℃ 0.08-5.68µ8/ml to ketoconazole and 0.006-0.71µg/ml to itraconazole. Significant reduction of MIC was observed at 37℃ compared to 25℃. 3. Effect of container size on determination time and MIC: When incubating in 96–well microplate and 24-well macroplate, determination of MIC was possible in 4th to 6th days after inoculation in broth-containig 96-well microplate compared to 8th to 12th days in broth-containing 24-well macroplate. But no difference in MIC was observed between different container size. 4. Effect of media on MIC: When using broth as media, MIC were below 0.006-5.68µg/ml to ketoconazole, below 0.006-0.36µg/ml to itraconazole in broth-containg 24-well macroplate. When using agar as media, MIC were below 0.006-5.68 µg/ml to ketoconazole, below 0.006-5.68 µg/ ml to itraconazole in agar-containing 24-well macroplate. 5. These findings confirm that determination of MIC of dermatophtes by method of Granade and Artis is fast and simple technique for antifungal susceptibility test.