ABSTRACT
Microfluidic chip or lab-on-a-chip is a multidisciplinary cross-technology. Among them, organ-on-a-chip technology enables precise regulation of cells and microenvironment at micron level.This tecnology is expected to simulate in vivo human physiology and overcome the shortcomings of traditional animal models and cell culture techniques.In ophthalmology, organ-on-a-chip models are primarily focused on creating biomimetic models of the cornea, retina and posterior chamber to study diseases such as dry eye, glaucoma, age-related macular degeneration and diabetic retinopathy.In addition, continuous monitoring and real-time diagnosis of tear and intraocular fluid biomarkers using microfluidic chips have become a current research hot topic.The microfluidic chips also have a wide range of applications in drug analysis, drug development, and drug screening.This article reviews the recent progress and shortcomings of microfluidic chip in in vitro model construction, point-of-care testing and drug development, and discusses its future development in ophthalmology.
ABSTRACT
Cell biology experiments in space are indispensable for investigating the effects of microgravity environment on living organisms. As an important technological means of supporting life science researche, space cell bioreactor may directly influence the data quality of space cell biology experiments and research level. To date, space cell bioreactor techniques are still under development, and lack of standard rationale. In this article, the technical progresses of space cell bioreactor were reviewed, by introducing the operational principle of several typical space cell bioreactors, analyzing the mode of culture medium supplying and character of fluid mechanics environment in space, as well as the relevant supporting techniques about the parametric controlling on temperature, dissolved oxygen and pH value and on-line microscopic imaging, so as to discuss the future perspective about space cell bioreactor techniques.