ABSTRACT
Objective To explore the advantages of automatic chromosome harvester in lymphocyte micronucleus test. Methods The peripheral blood of 80 radiation workers was collected, then lymphocytes were harvested with an automatic chromosome harvester and manual methods. After Giemsa stained the slides, the micronucleus of the lymphocytes was analyzed under a microscope. Results For lymphocytes from the same batch of specimens, the time of the automatic harvester (185 min) was less than that of the artificial group (350 min). The micronucleus rate (1.04‰ ± 0.63‰), micronucleus cell rate (0.89‰ ± 0.69) and micronucleus positive rate (70.0%) that obtained by the harvester were all higher than manual method (0.60‰ ± 0.68‰, 0.51‰ ± 0.55‰, 48.8%), and the difference was statistically significant (P < 0.01). Conclusion The automatic chromosome harvester can shorten the experimental operation time, reduce the workload of laboratory personnel, and protect the physical and mental health of laboratory personnel. At the same time, it can increase the detection rate of lymphocyte micronucleus, which can provide more accurate detection data for the clinic.
ABSTRACT
Objective To quantitatively investigate the effect of low-dose ionizing radiation on the frequencies of chromosome aberrations and micronucleus-containing cells of radiation workers.Methods Nine electronic databases were systematically searched on the basis of the published studies evaluating the effects of low-dose ionizing radiation on the frequencies of chromosome aberrations and micronucleuscontaining cells.Of the 195 studies searched,21 studies were identified with a total of 1 970 626 cells under studying.Cochrane' s Q and I2 statistics were used to evaluate heterogeneity among studies and pooling odds ratio (OR) with 95％ confidence intervals (CI) were calculated using random-effect models or fixed-effect models,and publication bias were also calculated.Meta-analysis was performed using Stata 12.0.Results The pooling OR of chromosome-type aberrations [OR =3.03 (2.59,3.56)],dicentric plus centric rings [OR =4.12 (2.99,5.67)],translocations [OR =2.73 (1.67,4.46)],micronucleuscontaining cells [OR =1.70 (1.40-2.06)] were higher for radiation workers when compared with control group.Conclusions The frequencies of chromosome aberrations and micronucleus cell of peripheral lymphocytes are significantly high in radiation workers who were occupationally exposed to low-dose ionizing radiation.It should be noted that the radiation protection of radiological workers be enhanced.