Serological Evaluation of Clinically Suspected Leptospirosis Cases in a Tertiary Care Hospital

Leptospirosis is a zoonotic infection caused by the spirochaete Leptospira, which has worldwide distribution. Laboratory diagnosis is routinely performed by serological tests like dipstick assay, lateral flow assay and latex agglutination which are rapid tests recommended for screening the disease. Microscopic Agglutination Test (MAT) is the standard test for serological diagnosis of leptospiral infection which is not included in the test panel in most of the peripheral laboratories as the procedure is laborious and it requires to maintain live leptospira. Therefore, one of the rapid tests is routinely employed for demonstration of leptospiral antibodies. Our objective was to screen the acute cases of leptospirosis by Leptochek WB IgM and PanBio IgM Elisa and compare the findings with the MAT and correlate the clinical findings with the serological tests.METHODSThis study was conducted in a tertiary care hospital in Mangalore from August 2010 to September 2013. A total of 314 cases of clinically suspected leptospirosis were included based on the Faine’s criteria. Patients’ serum was screened for leptospiral antibodies by Leptochek WB IgM, PanBio IgM ELISA and Microscopic Agglutination Test (MAT).RESULTSOut of 314 clinically suspected cases screened, seropositivity for leptospirosis by Leptochek WB-IgM, PanBio IgM ELISA and MAT was found to be 49 (15.6%), 65 (20.7%) and 78 (24.8%) respectively. Thus, an overall prevalence rate of leptospirosis was 24.8% (78/314) based on the MAT test. Sensitivity, specificity, positive predictive value and negative predictive value of Leptocheck WB IgM was 53.8%, 97%, 85.7 and 86.4% with MAT while the comparative values of PanBio-ELISA IgM with respect to MAT test was 74.5%, 97 %, 89.2% and 92% respectively. Common clinical features among MAT positive cases were fever, chills and rigors, oliguria, vomiting, jaundice and headache.CONCLUSIONSMAT is a standard serological test for Leptospirosis. This test is not always available for peripheral health centres, as the test is time consuming and cumbersome. Thus, screening tests are now being employed for screening the patients. Rapid tests like Leptocheck -WB can be supplemented with an ELISA test for screening of clinically suspected cases of Leptospirosis and later confirmed with the MAT at reference centres.

Imunodiagnóstico da leptospirose humana. 2. Estudo comparativo das reações de soroaglutinação microscópica, hemaglutinação passiva e imunofluorescência indireta / Immunodiagnosis of human leptospirosis. 2. Comparative study of microscopic agglutination passive hemagglutination and indirect immunofluorescence tests

Foram realizadas provas de hemaglutinação pa.ssiva e de imunefluorescêncía indireta em 98 soros obtidos de 38 pacientes com leptospirose causada presumivelmente pelos soro tipos icterohaemorrhagiae, copenhageni, grippotyphosa ou pomona. Hemácias de carneiro. sensibilizadas com antígeno polissacarfdico F" obtido de leptospira do. sorotípo patoc, foram utilizadas na hemaglutinação passiva. Para a prova de imunofluorescência indireta foram empregados antígenoa íntegros do. mesmo. sorotipo. Os resultados foram comparados com os obtidos através da prova de aglutinação microscópíca, na qual foram empregados .10 soro tipos patogênicos. As reações de hemaglutinação passiva e de imunofluorescência indireta mostraram ser gênero. específicas, sendo. que a primeira caracterizou-se por ser a mais indicada para o. diagnóstico. precoce da leptospirose humana aguda (AU).