Raman Spectroscopy of six explanted acrylic hydrophobic foldable intraocular lenses with glistening

Purpose: To study and interpret Raman spectra of six explanted acrylic hydrophobic foldable intraocular lenses (HFIOLs) with grade six microvacuoles and to understand the possible mechanism for microvacuole formation. Methods: Clinical data, slit-lamp photographs, and optical microphotographs of the explanted analytes were obtained. RS of the analytes were registered using a confocal Raman microscope (Lab RAM HR Evolution, Horiba Jobin Yvon) and Horiba Lab Space 6 Spectroscopy Suite software. Data were interpreted by identifying the functional group and fingerprint region of the spectra about the available literature. Results: IOLs were explanted for visual impairment after an average interval of 11.2 years following implantation. Each of the HFIOLs exhibited distinctive and identical Raman bands at the frequency range of 200–1,800, 2,600–3,000, and 3,200–3,700 cm?1 which were identified with those reported in the literature. The unique bands and peaks of the spectra were specific to the functional groups, its ring and other stretching variations, hydroxyl group, and water molecule. A spike at 1,640 cm?1 revealed the presence of monomer and indicated material bioincompatibility of the samples. Conclusion: Raman spectroscopy (RS) was found specific and an effective tool to detect the material change in the HFIOL and constituents of polymer biomaterial about microvacuole formation and also suggested modification and development of a more biocompatible and non-biodegradable polymer blend where RS could be a monitoring tool.

Correlación clínico-patológica de las Microvacuolas o ‘Glistenings’ en 100 lentes intraoculares (Lios) Acrysof Explantados / Clinicopathological correlation of microvacules or ‘glistenings’ in 100 explanted AcrySof intraocular lens (IOLs)

Resumen Propósito: analizar los LIOs acrílicos hidrofóbicos AcryS of de nuestro banco de lentes explantados para establecer la presencia de microvacuolas(glistenings), determinar su frecuencia y definir si existe una correlación con los datos clínicos para cada uno.Materiales y Métodos: se realizó análisis clínico-patológico de todos los lentes hidrofóbicos AcrySof® (n=100) que hacen parte del banco de más de 5.000 explantes de nuestro laboratorio. Se efectuó evaluación microscópica y se determinó la presencia de las microvacuolas; adicionalmente se estimó la severidad de las mismas y se hizo una correlación con los datos clínicos para cada lente.Resultados: el análisis foto microscópico reveló la presencia de microvacuolas en 43 de los 100 LIOs evaluados: las microvacuolas fueron clasificadas como...

Purpose:To analyze the AcrySof hydrophobic intraocular lens from our database in order to establish the presence of the microvacuoles (glistenigs), determine its frequency, and define clinicopathological correlation in each case.Materials and Methods: A clinicopathological analysis was performed in all the explanted AcrySof IOLs (n=100) that were accessioned in ourlaboratory database of more than 5,000 explants. Applying photo microscopy, we estimated the number of ‘glistenings’cases, evaluated their severity, and correlated them with clinical findings.Results: The laboratory analysis revealed microvacules in 43 out of the 100 evaluated lenses. Of those cases, 14 were mild, 17 were moderate, and 12 were marked. Of the 43 lenses in which the vacuoles were identified, only 6 had been diagnosed clinically...