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OBJECTIVES@#To compare the application effect of microwave digestion - vacuum filtration - automated scanning electron microscopy (MD-VF-Auto SEM) method and plankton gene multiplex PCR system in the diagnosis of drowning.@*METHODS@#Lung, liver and kidney tissue of 10 non-drowning cases and 50 drowning cases were prepared for further MD-VF-Auto SEM method analysis and plankton gene multiplex PCR system analysis. The positive detection rate of the two methods in each tissue was calculated.@*RESULTS@#The positive rate of the MD-VF-Auto SEM method detecting diatoms in drowning cases was 100%, and few diatoms were detected in the liver and kidney tissues of 6 non-drowning cases. By using the plankton gene multiplex PCR system, the diatom positive rate of drowning cases was 84%, and all the non-drowning cases were negative. There were significant differences in the positive rate of the liver, kidney tissues between MD-VF-Auto SEM method and plankton gene multiplex PCR system (P<0.05), as well as the total positive rate of cases. However, no significant differences were found in the positive rates of lung tissues (P>0.05).@*CONCLUSIONS@#MD-VF-Auto SEM method is more sensitive than plankton gene multiplex PCR system in diatom test. But the plankton gene multiplex PCR system can also detect plankton other than diatoms. Combination of the two methods can provide a more reliable basis for the diagnosis of drowning.
Subject(s)
Humans , Diatoms/genetics , Drowning/diagnosis , Liver , Lung , Microscopy, Electron, Scanning , Multiplex Polymerase Chain Reaction , Plankton/geneticsABSTRACT
OBJECTIVES@#To retrospectively analyze diatom test cases of corpses in water and discuss the value of quantitative analysis of diatoms in the diagnosis of drowning.@*METHODS@#A total of 490 cases of water-related death were collected. They were divided into drowning group and postmortem immersion group according to the cause of death. Diatoms in lung, liver, kidney tissue and water sample were analyzed quantitatively by microwave digestion-vacuum filtration-automated scanning electron microscopy (MD-VF-Auto SEM) method. The ratios of content of diatoms in lung tissue and water sample (CL/CD) were calculated.@*RESULTS@#The results of diatom test for three organs (lung, liver and kidney) were all positive in 400 cases (85.5%); the content of diatom in lung, liver, kidney tissues, and water samples of drowning group were (113 235.9±317 868.1), (26.7±75.6), (23.3±52.2) and (12 113.3±21 760.0) cells/10 g, respectively; the species of diatom were (7.5±2.8), (2.6±1.9), (2.9±2.1) and (8.9±3.0) types, respectively; the CL/CD of drowning group and postmortem immersion group were (100.6±830.7) and (0.3±0.4), respectively.@*CONCLUSIONS@#Quantitative analysis of diatoms can provide supportive evidence for the diagnosis of drowning, and the parameter CL/CD can be introduced into the analysis to make a more accurate diagnosis of drowning.
Subject(s)
Humans , Autopsy , Diatoms , Drowning/diagnosis , Lung , Retrospective Studies , WaterABSTRACT
Objective@#To establish a microwave digestion-inductively coupled plasma mass spectrometry assay for simultaneous determination of multiple elements in placenta.@*Methods@#Fresh placental tissues were dried at 60 ℃ for 15 h and ground into power. Then, 0.3 g homogenized samples were digested in a microwave digestion system. The interference from mass spectrometry was removed using the kinetic energy discrimination model in the inductively coupled plasma mass spectrometry, and the baseline interference was removed by online internal standards. The contents of 17 elements were determined in placental specimens using the established microwave digestion -inductively coupled plasma mass spectrometry assay, including V, Ni, Co, Fe, Cr, Cu, Zn, Mn, As, Sn, Sb, Ba, Se, Cd, Pb, Hg and Tl.@*Results@#Good linearity was shown for V, Ni, Co, Cr, As, Se, Cd, Pb and Tl at 1 to 50 µg/L, Fe at 100 to 5 000 µg/L, Zn, Cu, Mn and Ba at 10 to 500 µg/L, Sn and Sb at 0.1 to 5 µg/L, and Hg at 0.2 to 2 µg/L, with all correlation coefficients of 0.999 8 and higher. The detection limits of these 17 elements ranged from 0.5 to 100 μg/kg, with relative standard deviations of 2.1% to 6.5%, and recovery rates of 83.3% to 110.0%. The determination results of 17 elements were all within the normal reference range defined in the certified reference materials of chicken (GBW10018). @*Conclusions@#The microwave digestion-inductively coupled plasma mass spectrometry established based on optimized pretreatments and mass spectrometry detection conditions, is feasible for simultaneous determination of multiple elements in placenta.
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OBJECTIVE：To establish the method for the content determination of inorganic elements in Cyperus rotundus ，and to compare the contents of 14 kinds of inorganic elements in C. rotundus from different producing areas ，and to provide theoretical basis for its quality control and high quality resources development . METHODS ：The samples were processed by microwave digestion，and ICP-MS method was used to determine the contents of Na ，Mg，K，Ca，Mn，Fe，Ni，Cu，Zn，As，Se，Sr，Cd and Pb. SPSS 23.0 software were used for principal component analysis （PCA）and cluster analysis. RESULTS ：The average contents of above 14 kinds of inorganic elements in C. rotundus from different producing areas were 168.62，753.71， 6 938.33，24.31，14.69，197.77，0.60，2.43，26.89，0.21，0.06，5.64，0.05，0.32 mg/kg，respectively. The results of PCA showed that the cumulative variance contribution rate of the first four principal components was 86.203％，which could reflect most of the information of the original data. C. rotundus from Shandong ，Jiangxi，Shanxi，Hubei and Yunnan ranked the top five places in terms of comprehensive score of inorganic element contents. The results of cluster analysis showed that the samples from 9 producing areas were clustered into 5 categories，showing the characteristics of clustering by producing area. From the perspective of inorganic elements ，the quality of C. rotundus from East China ，Central China ，North China and Southwest China was better than that from South China. CONCLUSIONS ：Essential trace elements like Na ，Mg，K，Ca，Mn，Fe，Cu，Zn，Sr are rich in C. rotundus，and there are small amounts of Ni ，As，Se，Cd，Pb elements in it. The contents difference of inorganic elements in C. rotundus from different origins may related to the geographical area it belongs to.
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Objective To evaluate the transdermal safety of lanthanum (La) in sunscreen and jellyfish sting protective lotion, establish a microwave digestion-inductively coupled plasma opticalemission spectroscopy (ICP-OES) method for determination of lanthanum (La) in rat’s whole blood. Methods The whole blood samples were digested by microwave and analyzed by inductively coupled plasma emission spectrometer (ICP-OES). Using 333.749 nm as the analysis line, the content of La in rat whole blood was determined. Results The correlation linearity of the standard curve of this method was good (r>0.9994), the detection limit of the method was 0.0025 μg/ml, the limit of quantification was 0.0077 μg/ml, the precision was less than 3%, and the recovery rate was between 94.9% and 102.0%. Conclusion The ICP-OES method based on microwave digestion is stable and reliable, and can provide an important basis for the study of the transdermal safety of lanthanum.
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Objective@#To establish analytical method for the determination of vanadium ( V ), chromium ( Cr ), nickel ( Ni ), selenium ( Se ) and arsenic ( As ) in calcium based toothpastes by microwave digestion-inductively coupled plasma tandem mass spectrometry ( ICP-MS/MS ).@*Methods@#The 21 calcium based toothpaste samples from supermarkets and shops in the urban areas of Hangzhou were digested by 6 mL HNO3 and 1 mL H2O2 in microwave digestion system. Then He-SQ mode and O2-MS/MS mode of ICP-MS/MS were respectively used for the determination of Ni and V, Cr, Se, As. Indium ( In ) was used as internal standard for calibration. @*Results@# Good linear relationships were obtained for these five elements from 1.0 to 32.0 μg/L, with the correlation coefficients ranged from 0.999 3 to 1.000 0. The detection limits of the method ranged from 0.000 25 to 0.006 08 mg/kg. The recovery rates of standard spiking were 80.7%-105.7% when set at 0.4, 0.8 and 1.6 mg/kg, the recovery of standard reference material was 102.2%, and the relative standard deviations were 2.6%-4.8%. The concentrations of V, Cr, Ni, Se and As in 21 calcium based toothpaste samples were 0.024-1.935 mg/kg, 0.085-5.759 mg/kg, 0.090-3.673 mg/kg, <0.002 72-0.016 mg/kg and <0.006 08-0.321 mg/kg.@*Conclusion@#Microwave digestion-ICP-MS/MS can effectively reduce the interferences of polyatomic ions and doubly charged ions from the matrix, which is suitable for the determination of V, Cr, Ni, Se and As in calcium based toothpaste.
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OBJECTIVE: To establish a method for simultaneous determination of multiple metal elements in Millettia speciosa Champ. METHODS: Eight kinds of metal elements, i.e., Fe, Mn, Cu, Zn, Pd, Cd, As, and Se in Millettia speciosa Champ from different origins were determined by microwave digestion and ICP-MS. RESULTS: The contents of Fe, Mn, Cu, Zn, Pd, Cd, As, and Se in Millettia speciosa Champ from different origins were slightly different, the contents of Fe, Mn, Cu, and Zn were all above 1 mg·kg-1, while the contents of Pd, Cd, As and Se were mostly less than 1 mg·kg-1. The linear correlation coefficient of each metal element was 0.999 2-1.000 0, the average recovery was 87%-110.8%, and the RSDs of the reproducibility test was less than 10%. CONCLUSION: A microwave digestion and ICP-MS method for simultaneous determination of multiple metal elements in Chinese medicine Millettia speciosa Champ is established. The method is simple, sensitive, precise and repeatable, which can be used to determine the contents of metals in Millettia speciosa Champ.
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Objective: To elucidate the effect of sulfur fumigation on the content of chemical composition and quality of Panacis Quinquefolii Radix, and to discuss the identification method of sulfur fumigated Panacis Quinquefolii Radix. Method: The content changes of ginsenoside Rb1, Re, Rg1 in Panacis Quinquefolii Radix were determined by HPLC-DAD before and after sulfur fumigation. The content changes of 11 inorganic elements in Panacis Quinquefolii Radix before and after sulfur fumigation were detected by microwave digestion method and inductively coupled plasma emission spectrometry tandem mass spectrometry (ICP-MS). The quality difference of Panacis Quinquefolii Radix before and after sulfur fumigation was qualitatively distinguished combined with the principal component analysis. Result: Three ginsenosides were well separated and showed good linearity with R2 ≥ 0.999.Before sulfur fumigation, the contents of ginsenoside Rg1, Re and Rb1 in Panacis Quinquefolii Radix were (0.897±0.045), (13.092±0.639), (12.896±0.430) g ·kg-1, respectively. After sulfur fumigation, the contents of these three components in Panacis Quinquefolii Radix were (0.807±0.032), (11.968±0.627), (11.752±0.696) g ·kg-1, respectively. After fumigation, the contents of inorganic elements (Cr, Mn, Co, Cu, As, Se, Ag, Cd, Sb, Pb and Fe) from Panacis Quinquefolii Radix increased in varying degrees. According to the distribution results of three-dimensional (3D) scattered points, sulfur and non-sulfur fumigation products of Panacis Quinquefolii Radix could be effectively distinguished. Conclusion: After sulfur fumigation, the contents of ginsenoside Re and Rb1 in Panacis Quinquefolii Radix decrease significantly, but the contents of 11 inorganic elements increase, suggesting that sulfur fumigation may have a great influence on the quality of Panacis Quinquefolii Radix.
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Objective To analyze and discuss the content determination of As and Hg from Lilium Lancifolium in Longshan County; To optimize microwave digestion conditions. Methods Automatic microwave digestion appratus was used. Lilium Lancifolium samples from Longshan County were digested in teflon microwave tube with HNO3-H2O2, and As and Hg were measured with atomic fluorescence spectrometry (AFS). Results The content of As was in the range of 0.031–0.507 mg/kg, and the highest content of Hg was 0.024 mg/kg. The regression equation was Y=221.23X+170.72(r=0.995 9),Y=503.52X-682.43,(r=0.999 2).For the production base,the recoveries of As and Hg were 94.32% and 92.48% in the samples, and RSD were 2.14% and 2.70%; for the breeding base, the recoveries of As and Hg were 94.95% and 93.52% in the samples, and RSD were 1.15% and 1.97%. Conclusion The method is simple and reliable, which can be used to the content determination of As and Hg from Lilium Lancifolium, and provide references for the choice of base of production and breeding of Lilium Lancifolium in Longshan County.
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OBJECTIVE:To establish a method for the content determination of 8 metal elements in Propylgaclate and sodium chloride injection. METHODS:Microwave digestion-inductively coupled plasma mass spectrometry(ICP-MS) was adopted. Radiofrequency power was 1 530 W;cooling temperature was 4 ℃;collision gas was He gas;carrier gas was argon;flow rate of carrier gas was 1.08 L/min;integration time was 0.3 s;plasma gas flow rate was 15 L/min;the vacuum degree of quadrupole was 3.04×10－4 Pa;sampling cone aperture was 1.0 mm;interception cone aperture was 0.4 mm;the speed of sampling was 0.3 rps;data collection was repeated for 3 times. The microwave digestion power is 1 600 W,and the heating process is heated to 160℃at room temperature for 30 min,and maintained at 5 min,and then heated to 190 ℃ at a temperature of 5 ℃/min and maintained 45 min. RESULTS:The linear range of Mg and Al were 1-250 ng/mL;the linear range of Cr,Mn,Fe,Cu,Zn,Cd were 1-100 ng/ mL(all r≥0.999 0). The limits of detection were 0.063 6-1.785 0 ng/mL. RSDs of precision,stability and reproducibility tests were all lower than 4%. The recoveries were 89.65%-105.60%(RSD were 1.57%-3.98%,n＝9). RSDs of durablity were all lower than 12%. CONCLUSIONS:The method is simple,accurate,precise,stable,reproducible and durable. It can be used for content determination of 8 metal elements in Propylgaclate and sodium chloride injection.
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OBJECTIVE: To establish the method for content determination of 12 trace elements (Cu, Mn, Mg, Cd, Zn, Fe, Ba, Se, Pb, As, Cr, Ni) in Shiquan dabu pills, and to provide reference for further improvement of its quality control.METHODS: ICP-OES was adopted to detect. Microwave digestion was used for sample pretreatment with digestion power of 800 W; digestion process included heating to 120 ℃ keeping for 3 min, heating to 160 ℃ keeping for 5 min, and finally heating to 180 ℃ keeping for 20 min. PF power was 1 150 W, and pump speed was 50 r/min; the flow rate of atomizing gas was 0. 5 L/min, and that of plasma gas was 12. 0 L/min, detection wavelength of 182. 205-455. 403 nm. RESULTS: Results of 12 elements determination were all within standard range according to national standard substance sampling determination. The liner ranges of Cu, Mn, Mg, Cd, Zn, Fe, Ba, As, Cr and Ni were 0. 2-2. 0 mg/L, those of Se and Pb were 0. 4-4. 0 mg/L (r≥0. 999 5). The detection limits ranged 0. 1-8. 5 μg/L, and limits of quantitation were 0. 3-28. 3 μg/L. RSDs of precision tests were all lower than 1. 00％ (n=6). The average recovery rates were 88. 5％-99. 0％ (RSD were 1. 7％-4. 9％, n=6). CONCLUSIONS: The method is rapid, simple and sensitive, and it can be used for content determination of 12 trace elements in Shiquan dabu pills.
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Objective To study a method for determination of aluminum in food,and to ensure food safety and public health.Methods Microwave digestion-chrome azurol S spectrophotometric method was used to detect the aluminum content in fried dough sticks.Detection limit,linear range,precision,accuracy and maximum absorption wavelength were calculated.Results The method detection limit was 0.08 μg,precision was 0.60％-5.90％,and recovery rate was 96.0％-102.3％,maximum absorption wavelengnth was 620 mm.Aluminum content in 10 samples of fried dough sticks were all exceeded the standard,which were 709.5,980.8,1 010.3,1 151.5,1 214.0,1 243.7,1 318.1,1 374.0,1 435.0 and 1 479.4 mg/kg.Conclusion The method is simple,reliable,reproducible and easy to be used.
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Objective To determine the hair selenium level by material microwave digestion-atomic fluorescence spectrometry. Methods The selenium in hair was determined by hydride atomic fluorescence spectrometry after microwave digestion, and the method was improved by exploring the test conditions. The linear relationship and linear range of the standard curve, the detection limit, the precision and the accuracy of the sample determination were implemented under the optimized conditions. The selenium value of standard material of human hair was tested by material microwave digestion-atomic fluorescence spectrometry. Results The selenium detection limit of material microwave digestion-atomic fluorescence spectrometry was 0.095 μg/g, the correlation coefficient of standard curve was 0.9997, the relative standard deviation was 1.32% - 2.48%, the average recovery rate was 93.4% - 98.2%, and the selenium value of standard material of human hair was in the standard value range of uncertainty, which met the requirements. Conclusion The method is simple, accurate and precise, and it is an accurate and reliable method for determination of selenium in hair.
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Objective: To establish a method to determine 5 hazardous elements in animal traditional Chinese medicines (TCM) by microwave digestion-inductively coupled plasma mass spectrometry (ICP-MS).Methods: HNO 3-H 2 O 2 was used to decompose animal TCM, the working parameters of ICP-MS were optimized, and the matrix effect and the mass spectrum interferences were corrected by the in-line addition of Ge, In and Bi internal standard solution and the collided reaction cell technology (KED mode), respectively.Results: The detection limits of 5 hazardous elements were within the range of 0.04-0.52 μg·L-1 , the relative standard deviations (RSD) were within the range of 1.1%-4.8% , and the recoveries were within the range of 92.4%-110.0%.The method was applied to determine the 5 hazardous elements in 40 batches of animal TCM.The results indicated that some kinds of hazardous elements in animal TCM were high, and more attention should be paid to the problems in the production and use of animal TCM.Conclusion: The analysis method is simple, rapid and accurate, and suitable for the determination of hazardous elements in animal TCM.
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Objective: To establish an inductively coupled plasma mass spectrometry (ICP-MS) with microwave digestion method for the determination of 32 kinds of inorganic elements in Qingxue Bawei Tablets (QBT), including Be, B, Na, Mg, Al, P, K, Ca, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, As, Se, Rb, Sr, Mo, Ag, Cd, Sn, Sb, Cs, Ba, Hg, Tl, and Pb, in order to establish the fingerprint chromatogram of inorganic elements and analyze characteristic elements. Methods: The samples with nitric acid and hydrogen peroxide system as digestion reagents were digested via microwave, calibrated by internal standard elements, such as 6Li, Sc, Y, Rh, In, Ho, Bi, and Th. The contents of 32 inorganic elements were analyzed by ICP-MS. The content distribution curve of inorganic elements was plotted. And the principal components were analyzed with the SPSS 19.0 software. Results: The 32 inorganic elements showed good linearity in the selected concentration ranges, with the correlation coefficients over 0.999 5. The detection limits of the 32 elements were in the range of 0.001-6.390 μg/L. The RSD values of precision, stability, and repeatability all met the demands of quantitative analysis. The recovery was 94.36%-105.47%, while their RSD was 1.53%-4.56%. The fingerprint chromatogram was established by the content distribution curve of inorganic elements. Different batches of samples were similar peak shape, and the amount of inorganic elements content in different order tend to be consistent. The results of principal component analysis showed that Co, As, Ba, Ca, Sb, Ti, Ni, Fe, Cs, Se, P, Al, and K might be the characteristic elements in QBT. The content of five heavy metals (Pb, Cd, As, Hg, and Cu) was under the limit requirements of Chinese Pharmacopoeia (2015). Conclusion: The method is simple, rapid, and accurate, and it can be used for the content determination of inorganic elements in QBT. The analysis of inorganic elements can provide certain reference for the quality control, safety evaluation, and clinical application of QBT.
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The contents of five heavy metals (Cu, Pb, As, Cd, Hg) in 17 batches of Cordyceps sinensis were determined by microwave digestion-ICP-MS, and their distribution in C. sinensis were analyzed. The results showed that the contents of Cu, Pb, Cd and Hg in all batches were in accordance with the international standards of Chinese Medicine-Chinese Herbal Medicine Heavy Metal Limit, with their contents in the stroma higher than that in the caterpillar body, and the excess rate of As, which mainly concentrated in the caterpillar body part of C. sinensis, was 88.24%, as the content of As in the caterpillar body was 7 to 12 fold of that in the stroma. In this study, the distribution of five heavy metals in C. sinensis was clarified, and the existing problems of arsenic limit of heavy metal in C. sinensis were analyzed, and some suggestions were put forward. It is hoped that the reference standard can be provided for the limited standard of arsenic in C. sinensis.
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Objective: To establish the inductively coupled plasma mass spectrometry (ICP-MS) method for content determination of five kinds of heavy metals (copper, arsenic, cadmium, mercury, and lead) in Anoectochilus roxburghii. Methods: After the treatment of microwave digestion, ICP-MS method was used in the determination. Results: The standard liquid working curves of Cu, As, Cd, Hg, and Pb had good linearity, with the correlation coefficients of more than 0.998. The average recovery of Cu was 104.3% with relative standard deviation (RSD) of 4.0%, the average recovery of arsenic was 102.2% with RSD of 4.3%, the average recovery Cd was 100.5% with RSD of 0.4%, the average recovery rate of Hg was 101.0% with RSD of 3.6%, and the average recovery of Pb was 101.1% with RSD of 1.1%. Conclusion: The method is stable, reliable, fast, and accurate, and has high sensitivity and good reproducibility. It can be used for the simultaneous determination of Cu, As, Cd, Hg, and Pb in A. roxburghii.
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Objective: To quantify the contents of heavy metal elements in the roots of Chuanmingshen violaceum (Chuanmingshinis Violacei Radix, and Chuanmingshen) and to evaluate its safety. Methods: An inductively coupled plasma mass spectrometric (ICP-MS) method was developed to quantify six heavy metal elements, i.e. lead (Pb), cadmium (Cd), mercury (Hg), copper (Cu), arsenic (As), and chromium (Cr) in Chuanmingshinis Violacei Radix. A total of 33 Chuanmingshinis Violacei Radix samples were collected from eight counties/districts in Sichuan province of China, processed and then quantified for the contents of these elements. Moreover, the eight counties/districts were divided into four cultivation areas of Chuanmingshinis Violacei Radix based on the geographical location and ecological environment. The differences of heavy metals were compared by statistical analysis. Results: The total content of each element in the 33 samples of Chuanmingshinis Violacei Radix were Pb ≤ 0.256 μg/g, Cd ≤ 0.235 μg/g, Hg ≤ 0.123 μg/g, Cu ≤ 3.963 μg/g, and Cr ≤ 2.145 μg/g; As below the detection limit, the findings were in line with the "Green Trade Standards of Importing and Exporting Medicinal Plants and Preparations" in China. Comparing the amount of heavy metal elements in the samples of Chuanmingshinis Violacei Radix, it was found that the contents of the six heavy metal elements were varied among the four cultivation areas. Except for the difference of Pb content which was significant between the two cultivation areas (P < 0.05), other heavy metal elements in these samples among the four cultivation areas were not significantly different. Conclusion: The developed ICP-MS method is suitable to accurately quantify the content of heavy metal elements in the samplesof Chuanmingshinis Violacei Radix. On the basis of the level of heavy metal elements, Chuanmingshinis Violacei Radix is considered safe and the current cultivation areas are suitable for the cultivation of C. violaceum.
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OBJECTIVE: To establish a method for detecting tin in human blood by microwave digestion-atomic fluorescence spectrometry. METHODS: The microwave digestion instrument was used to digest 1. 00 mL blood sample with 4. 00 mL concentrated nitric acid( mass fraction 65. 00%) and 1. 00 mL perchloric acid( mass fraction 68. 00%). After 1. 00 mL of thiocarbamide-ascorbic acid( mass fraction 10. 00%) was added,hydrochloric acid( concentration 1. 5 mol / L) was added to a total constant volume of 10. 00 mL. Then 5. 00 mL of the sample was collected and detected by atomic fluorescence spectrophotometry. RESULTS: The good linear relationship was shown in the range of 0. 00-60. 00 μg / L,and the correlation coefficient was 0. 999 5. The limit of detection was 0. 01 μg / L. The average recovery rate was 98. 80%-103. 30%. The relative standard deviation( RSD) of within-run precision was 3. 74%-5. 26%,and the RSD of betweenrun precision was 1. 23%-4. 45%. The samples can be stored for at least 7 days under the temperature of 4 ℃.CONCLUSION: This method has the advantages of low detection limit,good precision and high sensitivity,which is suitable for the determination of tin in human blood.
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OBJECTIVE:To establish a method for simultaneous determination of the contents of Cu,Pb,As,Cr,Cd,Ba, Mn,Sb and Hg in Ganmaoling granule. METHODS:ICP-MS was conducted. The sample was handled by nitric acid microwave di-gestion system,the mass concnetration of 9 metal elements were determined by ICP-MS with Ge,In,Bi as internal standard and standard substance of test element standards. RF power was 1 100 W,sampling depth was 8 mm,carrier gas was argon(Ar)with high purity and flow rate was 1.0 L/min. RESULTS:All 9 elements had good linear range between mass concentration and ion peaks (r=0.999 1-0.999 9),detection limits were in the range of 0.3-6.0μg/L;RSDs of precision,stability and reproducibility tests were no more than 6.0%;average recoveries were in the range of 80.0%-113.5%,and RSDs were in 1.0%-4.5%. CONCLUSIONS:The method is simple,rapid and accurate,and can be used for the determination of 9 metal elements in Ganmaoling granule.