ABSTRACT
Objective@#To investigate the expressions of migration and invasion inhibitory protein (MIIP) and p21-activated kinase 1 (PAK1) in endometrial carcinoma (EC) and their correlation with clinicopathological features.@*Methods@#The protein levels of MIIP and PAK1 in 135 paraffin-embedded EC tissues, 55 atypical hyperplasia of endometrium (AHE) and 88 normal endometrium (NE) tissues were quantified by immunohistochemistry, the clincial significance and the relationship of these two proteins were also analyzed.@*Results@#The positive rates of MIIP expression in NE, AHE and EC tissues were 52.3%(46/88), 41.8% (23/55) and 34.8% (47/135), respectively. The expression of MIIP in EC was significantly lower than that of MIIP in NE (P<0.05). The positive rates of PAK1 expression in NE, AHE and EC tissues were 45.5% (40/88), 50.9% (28/55) and 62.2% (84/135), respectively. The expression of PAK1 in EC tissues was significantly higher than that of PAK1 in NE tissues (P<0.05). The expression of MIIP in EC tissues was significantly associated with myometrial invasion, International Federation of Gynaecology and Obstetrics (FIGO) stage and lymph node metastasis (P<0.05). The expression of PAK1 in EC tissues was significantly related with differentiation, myometrial invasion, FIGO stage and lymph node metastasis (P<0.05). The expressions of MIIP and PAK1 in EC tissues were marginally related with the overall survival of patients (P=0.092, P=0.052). The expression of MIIP in EC was negatively correlated with PAK1 (r=-0.329, P<0.001).@*Conclusions@#The down-regulation of MIIP and up-regualtion of PAK1 paticipate in the initiation and development of EC, which are correlated with the poor prognosis of EC. The protein expression of MIIP is inversely related with PAK1 in EC.
ABSTRACT
Migration and invasion inhibitory protein(MIIP)inhibits cell proliferation,migration,and invasion,impeding tumorigenesis and tumor progression,by interacting with insulin-like growth factor binding protein 2(IGFBP2),histone deacetylase 6(HDAC6),p21 activated kinase 1(PAK1),epidermal growth factor receptor(EGFR),cell division cycle 20(CDC20),and topoisomerase.Recent studies revealed potential roles of MIIP in viral infection and cellular immunity.MIIP has become a research hotspot owing to its involvement in multiple signaling pathways and as a potential therapeutic target for tumors.This review summarizes the characteristics,functions, clinical significance,and possible pathogenic mechanisms of MIIP in multiple carcinomas.
ABSTRACT
Objective To investigate the expression of migration and invasion inhibitory protein (MIIP) in gastric adenocarcinoma and its clinicopathological significance.Methods The expression of MIIP was detected in 90 samples of gastric adenocarcinoma and 90 samples of normal gastric tissues by tissue microarray and immunohistochemical method.The correlation of MIIP expression with clinicopathological parameters and prognosis of gastric adenocarcinoma patients was analyzed.Results The expression level of MIIP in gastric adenocarcinoma tissues was significantly lower than that in normal gastric tissues [30.0％ (27/90) vs.55.6％ (50/90),x2 =12.006,P =0.001].The down-regulated expression of MIIP in gastric adenocarcinoma tissues was significantly associated with larger tumor mass (x2 =4.116,P =0.042),higher T stage (x2 =6.752,P =0.009),lymph nodes metastasis (x2 =7.234,P =0.007) and higher TNM stage (x2 =7.608,P =0.006).The 5-year overall survival rate was significantly higher in patients with high expression of MIIP than those with low expression (x2 =11.378,P =0.001).The tumor mass (OR =0.570,P =0.043),T stage (OR =0.213,P =0.036) and the expression level of MIIP (OR =1.967,P =0.042) were the independent predictors for prognosis of gastric adenocarcinoma.Conclusions The low expression level of MIIP is associated with poor prognosis in gastric adenocarcinomas.